LOCUS       p3E-2a-nlstdTomato        4465 bp    DNA     circular     25-JUN-2015
Created: Friday, January 28, 2005 2:27 PM
FEATURES             Location/Qualifiers
     misc_feature    268..295
                     /note="T2"
     misc_feature    427..470
                     /note="rrnB T1"
     misc_feature    537..552
                     /note="M13 Forward "
     misc_feature    591..747
                     /note="attP2R"
     misc_feature    2574..2669
                     /note="attP3 "
     misc_feature    complement(2730..2744)
                     /note="M13"
     misc_feature    2859..3668
                     /note="Kan"
     misc_feature    3789..4462
                     /note="pUC origin"
     misc_feature    840..878
                     /note="myc tag 1"
     misc_feature    879..917
                     /note="myc tag 2"
     misc_feature    918..956
                     /note="myc tag 3"
     misc_feature    957..995
                     /note="myc tag 4"
     misc_feature    996..1034
                     /note="myc tag 5"
     misc_feature    1059..1100
                     /note="myc tag 6"
     misc_feature    1113..1136
                     /note=""
     misc_feature    1137..2564
                     /note="tdTomato (2X)"
     misc_feature    1116..1136
                     /note="nuclear localization signal"
     misc_feature    750..815
                     /note=" 2A peptide"
     source          1..4465
                     /dnas_title="p3E-2a-nlstdTomato"
ORIGIN      
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCTG CAGCTCTAGA GCTCGAATTC TACAGGTCAC
      601 TAATACCATC TAAGTAGTTG ATTCATAGTG ACTGCATATG TTGTGTTTTA CAGTATTATG
      661 TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT
      721 CTCGTTCAAC TTTCTTGTAC AAAGTGGGAG GATCCGGAGC CACGAACTTC TCTCTGTTAA
      781 AGCAAGCAGG AGACGTGGAA GAAAACCCCG GTCCTGCAGG ATCCCATCGA TTtaaagcta
      841 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
      901 aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg
      961 agcaaaagct catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag
     1021 aggacttgaa tgaaatggag agcttgggcg acctcaccat ggagcaaaag ctcatttctg
     1081 aagaggactt gaatttagag atctcGAATT CcATGCCCAA GAAGAAGAGG AAGGtgatgg
     1141 tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
     1201 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1261 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     1321 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg
     1381 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1441 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca
     1501 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
     1561 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     1621 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct
     1681 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca
     1741 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc
     1801 accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct
     1861 cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg
     1921 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1981 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     2041 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     2101 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     2161 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     2221 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     2281 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     2341 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     2401 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     2461 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     2521 gccgccacca cctgttcctg tacggcatgg acgagctgta caagGGATCc taaCAACTTT
     2581 ATTATACAAA GTTGGCATTA TAAAAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT
     2641 CACTATCAGT CAAAATAAAA TCATTATTTG GAGCTCCATG GTAGCGTTAA CGCGGCCGCG
     2701 ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG
     2761 TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGAACAATAA
     2821 AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA
     2881 CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC
     2941 GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC
     3001 CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG
     3061 TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA
     3121 CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT
     3181 TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC
     3241 GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG
     3301 CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC
     3361 GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC
     3421 CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA
     3481 AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG
     3541 CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA
     3601 AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT
     3661 TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC
     3721 GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG
     3781 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
     3841 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
     3901 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
     3961 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
     4021 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
     4081 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
     4141 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
     4201 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
     4261 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
     4321 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
     4381 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
     4441 TTTGCTGGCC TTTTGCTCAC ATGTT
//

