LOCUS       pCS-flt4um159-HA        8298 bp    DNA     circular     25-JUN-2015
Created: Wednesday, January 12, 2005 1:34 PM
FEATURES             Location/Qualifiers
     misc_feature    104..107
                     /note="RfB"
     misc_feature    35..51
                     /note="SP6 promoter"
     misc_feature    64..78
                     /note="bglobin leader"
     misc_feature    4347..4542
                     /note="Poly A "
     misc_feature    complement(4629..4647)
                     /note="M13 Reverse"
     misc_feature    108..131
                     /note="attB1"
     misc_feature    4229..4253
                     /note="attB2"
     misc_feature    4287..4307
                     /note="attB3"
     misc_feature    4255..4285
                     /note="HA tag "
     CDS             4255..4285
                     /note=""
                     /translation="YPYDVPDYA"
     misc_feature    152..209
                     /note="exon1"
     misc_feature    210..306
                     /note="exon2"
     misc_feature    307..581
                     /note="exon3"
     misc_feature    152..177
                     /note="1054  attB1-kozak-flt4 cds GW  fw"
     misc_feature    152..4214
                     /note="full-length Flt4 short version cDNA"
     misc_feature    582..709
                     /note="exon4"
     misc_feature    710..884
                     /note="exon5"
     misc_feature    885..1027
                     /note="exon6"
     misc_feature    1028..1199
                     /note="exon7"
     misc_feature    1200..1317
                     /note="exon8"
     misc_feature    1318..1490
                     /note="exon9"
     misc_feature    1491..1656
                     /note="exon10"
     misc_feature    1657..1783
                     /note="exon11"
     misc_feature    1784..1883
                     /note="exon12"
     misc_feature    1884..2225
                     /note="exon13"
     misc_feature    1568..1570
                     /note="473:important for hetero-dimerization R2 and R3"
     misc_feature    1628..1630
                     /note="493"
     misc_feature    1691..1693
                     /note="514"
     misc_feature    2226..2372
                     /note="exon14"
     misc_feature    2373..2504
                     /note="exon15"
     misc_feature    2505..2611
                     /note="exon16"
     misc_feature    2612..2747
                     /note="exon17"
     misc_feature    2748..2852
                     /note="exon18"
     misc_feature    2853..2966
                     /note="exon19"
     misc_feature    2693..2695
                     /note="851:specific Y between zFlt4 and hFlt4"
     misc_feature    2967..3055
                     /note="exon20"
     misc_feature    3056..3161
                     /note="exon21"
     misc_feature    3162..3262
                     /note="exon22"
     misc_feature    3263..3385
                     /note="exon23"
     misc_feature    3386..3497
                     /note="exon24"
     misc_feature    3353..3355
                     /note="no RE site:1071 (interact with Crk, activate JNK
                     for cell survival)"
     misc_feature    3368..3370
                     /note="no RE site:1076 (interact with Crk, activate JNK
                     for cell survival)"
     misc_feature    3437..3439
                     /note="1099: conserved among zFlt4, hFlt4 and zKdrl"
     misc_feature    3498..3597
                     /note="exon25"
     misc_feature    3509..3511
                     /note="1123: conserved among zFlt4, hFlt4 and zKdrl"
     misc_feature    3598..3703
                     /note="exon26"
     misc_feature    3938..4023
                     /note="exon29"
     misc_feature    3704..3816
                     /note="exon27"
     misc_feature    3817..3937
                     /note="exon28"
     misc_feature    3818..3823
                     /note="no RE site:1226/1227 (interact with GRB, activate
                     ERK and Akt, required for sprouting)"
     misc_feature    4154..4156
                     /note="has specific site for RsaI:1338 (interact with
                     GRB, mild affect on sprouting, this phosphorylation is
                     blocked when form R2/R3 heterodimer)"
     misc_feature    4142..4144
                     /note="1334:specific Y between zFlt4 and hFlt4 althogh
                     the tyrosine is not phosphorylated in VEGF-C treated PAE
                     cells expressing Vegfr3 (Dixelius et al. 2003 JBC)"
     misc_feature    4187..4189
                     /note="1349: zFlt4 specific"
     misc_feature    4024..4214
                     /note="exon30"
     source          1..8298
                     /dnas_title="pCS-flt4um159-HA"
ORIGIN      
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCA AGGATCACAA GTTTGTACAA
      121 AAAAGCAGGC TCCGAATTCG CCCTTgccac cATGAAGAGA GATTTTACGT TTTTCTGTCG
      181 GATTTGGATT GGGATTCCCT TCTTCTCAGG TCTGGTGAAT GGGTTTTCTA TGAGTCCACC
      241 CACCCTTGAC AACACCAAAG ACCAGCTTGT GATTAACGCT AACGACACAC TGAACATTAC
      301 ATGCAGGGGT CAGCGAATTC TGGACTGGAG CTGGCCTGAA GAGTCTTTGA GTAAAGTGGA
      361 GTTTACTGAT CGCCAGGGTC AGCAGTCACC CACTGACACC CCAGGGTATA GAGAAATCAG
      421 GCTGAAGGAG TGTCAAGGGG TGGCTGGAAA ACCCTACTGC AAGATCTTAA TACTTACCAA
      481 TGCCCAGGCC AATGACTCGG GTTATTACCG CTGCTTCTAC AAGGACATTA AAGCTGTTAT
      541 TGATGGCACC ACGGCAGCCA GCATTTTTGT GTTTGTTCGA GACCCAGAGC ATCCATTCAT
      601 CAAAAGGGGA GACAACGACA TGGAGACCAT CTTCATAACA GACTCTGAAA CACACATCGA
      661 AGTACCATGT CTGGTTTCAG ATCCTGACCT AAAAGTCACT CTCTTCTCGT TAGTGCCGTA
      721 TCCAGAGCCT GTGGATGGCA GTGTGGTCAC CTGGAATAAT AAAAAGGGTT GGTCGATTCC
      781 CAGGCATATC ATTCAGAACA CTTCCACCTT TATTGGCTTC TACTGCTCCA TCTCAGTCCA
      841 AAACAGCCAG CACACATCAT CAATCTATGT TGTCCAGGTC ATTGGGCTGA AGTTTTATGA
      901 GTTCAAGCTG TTTCCTGAAG ACTCGCCTGT GGAGTTGATG CAGGGGGAGA GTTTGGTGCT
      961 CAACTGCACT GCACTGGTCG ACTTCAACAC TGGTGTGGAT TTCCAGTGGG ACTATCCAGG
     1021 CAAAAAGGAG AACCGGTTGG CGAGTTTACA GCCTCTGCGT AACGTCCTGG ATGAAGCCAC
     1081 GGAAATCTCC AGTATCCTCT CAATCAGAAA CATTCACTTG GATGATAGTG GCTACTACAC
     1141 CTGCTGGGCT AATACTCTGG AAATGAAACG AGAGCTCACT ACTGTAGTCA TAGTTCATGA
     1201 AAAACCTTTT ATCAGCTTGG ACTACAGGAA TGGATCAGTT ATAGAGGCAA AAGAGGGACA
     1261 AAAATCTGTC CGGCTATCCG TAAAAGTGTC AGCATACCCT TCACCAGAAA TACAATGGTA
     1321 TAAGAATGGA AAACTGATCA GCAGCAAGAA TTCAAGCAGG TTTAAGGTCC AGCAGCACTC
     1381 TCTACAGATC AGGGATGTGT GTAAGCAGGA TGCTGGAGAA TACATGCTGG TCCTGAAAAA
     1441 TAGCCCTGCA GCCCTGGAGA AAAGACTCAA CTTTACTCTC ATAGTCAACG TTCCACCTCA
     1501 GATTCATGAG AAGGAGGCAG CACCACCTAC AAACCTCTAT GGGAAGGGCA CTCGACAGAT
     1561 CCTCACTTGC ACCGCAGACG GGAGCCCTCC AGCCTCCATC AGCTGGCAGT GGAGGCCCTG
     1621 GAGCCCCTGC GATCTGGAGC GTACCCGCAG AGCCCTGAGA CGGAGGGGTG GACGAGACCA
     1681 GAGTCCTTTC TGTCACAACT GGATGGATTT GGACCCTGAA CATGCTGTAA ACCCTATTGA
     1741 GAGTATTGAC ACATTGACTC AGATGGTGGA CGGCAAAGAA AAGACTGTCG GCCGTGTGGT
     1801 TATTCAGAAT GCCAGTGTGC CAGCTATGTT GGCTGAGAAC AAAGTGGGAA AAGATGAACG
     1861 ACTGATTTAT TTCTACGTAA CCACTATCCC TGAAGGATTC GATATAGAGA TGGAGCCCTC
     1921 AGAGGATCCG CTTGAGCAGG ACCTGGTACA GCTGAAGTGT AATGCAGATA ATTTCACCTA
     1981 TGAGAACCTG CGGTGGTACC GCCTGGACCC GCAGACTGTT CCTCCAGAGC TGGACTGCAA
     2041 GAGTCTGCAC CAGTATGCCA CATTTTTGGA GGGACAACTA TCTTTTCAGA CCACCAGCAA
     2101 CAACTGGGTC CTGCAGCTTA ACATTACCAA CATTCAGCTA CAGGATGAGG GGAACTATGT
     2161 GTGTGAGGTG CAGAACCGAC GAACCGGGGT GAAACATTGT CACCGCAAAT ACATCCCAGT
     2221 CAAAGCAATG GAAGCACCTC GATACCGTCA CAACCCAACT AATCATACGG TAAATGTGAG
     2281 CGAGTCGCTG CAGATGAACT GTGATGTGGA AGGCACACCC TTTCCTCAGC TGTCCTGGTT
     2341 TAAAGATAAC CAACCCCTCC ATCAGATATC AGGGATCCTC CTTCAGGACT CCAATCGGAC
     2401 TCTCAGCATT CAGCGAGTGC GAGAGGAAGA CGCCGGTCTG TACACCTGCT CTGCCTGCAA
     2461 CCAGAAGGGC TGCGTTCAGT CCTCCGCCAC GGTGTCAGTG ATTGGCTCTG ATGACAAAAC
     2521 CAATGTAGAA ATAGTGATCC TCATCGGCAC AGGAGTCATC GCCATCTTTT TCTGGGTCCT
     2581 TCTTCTTGTC ATCTTCTGCA ATGTCAAGCG AGTGAACCCA GCTGATATCA AGACAGGCTA
     2641 TCTGTCCATC ATCATGGACC CGGGGGAAGT GCCGCTGGAG GAGCAGTGTG AATACCTGCC
     2701 GTACGACTCC AGCCAGTGGG AGATTTCCAG AGACAGACTG CGCCTCGGTA AGGTGCTCGG
     2761 CCATGGAGCT TTCGGGAAGG TGATTGAAGC CTCAATCTTT GGTCATGACA AGAAATCCTC
     2821 TGCAAACACA GTGGCTGTCA AGATGTTGAA AGAGGGAGCA ACAGCCAGCG AGCACAAAGC
     2881 CCTGATGTCA GAGCTGAAGA TCCTCATTCA TATTGGAAAC CATCTCAATG TTGTCAACCT
     2941 TCTTGGAGCT TGTACCAAAC CTAACGGTCC TCTCATGGTT ATAGTGGAGT ATTGTAAATA
     3001 TGGAAATCTG TCCAACTTCC TGCGTGCCAA GAGGGAGTTT TTTTTACCAT ACAGGGATCG
     3061 TTCTCCTAAA ACCCAAAGTC AAGTGCGACG GATGATAGAA GCAGGTCAGG CGAGCCAGAG
     3121 TGAGCATCAG CCGTCCACTT CCTCCACCAA CCCTCCACGT GTTACAGTGG ATGATCTCTG
     3181 GAAAACTCCA CTCACAATAG AGGATCTGAT ATGCTACAGT TTTCAAGTTG CACGAGGAAT
     3241 GGAGTTTCTG GCATCTCGTA AGTGTATTCA TCGAGATCTG GCAGCCCGAA ACATCCTCCT
     3301 GTCCGAGAAC AATGTGGTGA AGATCTGTGA CTTTGGTTTA GCACGGGACA TCTACAAAGA
     3361 CCCTGACTAT GTGCGCAAAG GCAATGCCAG GCTGCCGCTG AAGTGGATGG CTCCAGAGAG
     3421 CATCTTTGAT AAGGTTTACA CCAGTCAGAG TGACGTCTGG TCTTTTGGAG TTCTGCTCTG
     3481 GGAGATTTTC TCACTAGGAG CGTCTCCATA CCCCGGCATC CAGATTGATG AAGATTTCTG
     3541 CAAACGACTC AAAGACGGTA CCAGGATGAG AGCACCAGAC AATGCGTCCC CTGAAATATA
     3601 TGGCATCATG TTGGCCTGCT GGCAGGGCGA GCCCAGGGAG AGACCCACAT TTCCAGCTCT
     3661 GGTGGAGATA CTTGGAGATC TACTGCAGGA AAACAGTCTA CCAGAAATCC CGTTCAATGT
     3721 GTCTCAGAGC TCAGAGGACG ATGGCTTCTC ACAAGCTTCT TCCAGACCAC CATCACAAGA
     3781 GGAGATCAGA TTGGCCTGCA ACACACTGCC AACACGATAT TACAACTGCG TGCCGTTTGC
     3841 TGGCTGTGTG ATGGTCGGGC CCTCCAGCAC ATGTCATTCT CGAGTGAAGA CCTTTGAGGA
     3901 ACTTCCTATG GAAATGACAT CACACAAGAC ACAGCATGAC AGTCAGACAG ACAGTGGGAT
     3961 GGTTTTGGCA TCAGATGAAC TGGAGAGGTT TGAACACAAG CACAGAGGAG CCATGTTGAC
     4021 ACATGCGACG ACGGGCCAAA GCACTGAGCG CCTCATCAGT TGTCCATCGG TGAGCAGCAG
     4081 CGGCAGCGGT GGTGGTTTGC TCCGTCCAGT GTTCTTCACT CAACTCTCAG GCCAGACCTT
     4141 CTACAACAAC GAGTACGGTC ATCTGTCAGA GGAGGGCGTC AGCGACTACT TCTCCTCCTC
     4201 AGACCAGGCC GTTTaCAAGG GCGAATTCgA CCCAGCTTTC TTGTACAAAG TGGacTATCC
     4261 GTACGACGTA CCAGACTACG CATAACCAAC TTTATTATAC ATAGTTGcac TCGAGCCTCT
     4321 AGAACTATAG TGAGTCGTAT TACGTAGATC CAGACATGAT AAGATACATT GATGAGTTTG
     4381 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA
     4441 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC
     4501 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAATTCGCG GCCGCGGCGC
     4561 CAATGCATTG GGCCCGGTAC CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT
     4621 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
     4681 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
     4741 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
     4801 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
     4861 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
     4921 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
     4981 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
     5041 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     5101 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     5161 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     5221 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     5281 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     5341 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     5401 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     5461 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     5521 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     5581 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     5641 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     5701 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     5761 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     5821 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     5881 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC
     5941 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     6001 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     6061 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     6121 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     6181 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     6241 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     6301 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     6361 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     6421 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     6481 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     6541 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     6601 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     6661 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     6721 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
     6781 ACCTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC
     6841 TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC
     6901 GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC
     6961 TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA
     7021 CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG
     7081 AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG
     7141 AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC
     7201 ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CCCATTCGCC ATTCAGGCTG
     7261 CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GTCGACCATA
     7321 GCCAATTCAA TATGGCGTAT ATGGACTCAT GCCAATTCAA TATGGTGGAT CTGGACCTGT
     7381 GCCAATTCAA TATGGCGTAT ATGGACTCGT GCCAATTCAA TATGGTGGAT CTGGACCCCA
     7441 GCCAATTCAA TATGGCGGAC TTGGCACCAT GCCAATTCAA TATGGCGGAC TTGGCACTGT
     7501 GCCAACTGGG GAGGGGTCTA CTTGGCACGG TGCCAAGTTT GAGGAGGGGT CTTGGCCCTG
     7561 TGCCAAGTCC GCCATATTGA ATTGGCATGG TGCCAATAAT GGCGGCCATA TTGGCTATAT
     7621 GCCAGGATCA ATATATAGGC AATATCCAAT ATGGCCCTAT GCCAATATGG CTATTGGCCA
     7681 GGTTCAATAC TATGTATTGG CCCTATGCCA TATAGTATTC CATATATGGG TTTTCCTATT
     7741 GACGTAGATA GCCCCTCCCA ATGGGCGGTC CCATATACCA TATATGGGGC TTCCTAATAC
     7801 CGCCCATAGC CACTCCCCCA TTGACGTCAA TGGTCTCTAT ATATGGTCTT TCCTATTGAC
     7861 GTCATATGGG CGGTCCTATT GACGTATATG GCGCCTCCCC CATTGACGTC AATTACGGTA
     7921 AATGGCCCGC CTGGCTCAAT GCCCATTGAC GTCAATAGGA CCACCCACCA TTGACGTCAA
     7981 TGGGATGGCT CATTGCCCAT TCATATCCGT TCTCACGCCC CCTATTGACG TCAATGACGG
     8041 TAAATGGCCC ACTTGGCAGT ACATCAATAT CTATTAATAG TAACTTGGCA AGTACATTAC
     8101 TATTGGAAGG ACGCCAGGGT ACATTGGCAG TACTCCCATT GACGTCAATG GCGGTAAATG
     8161 GCCCGCGATG GCTGCCAAGT ACATCCCCAT TGACGTCAAT GGGGAGGGGC AATGACGCAA
     8221 ATGGGCGTTC CATTGACGTA AATGGGCGGT AGGCGTGCCT AATGGGAGGT CTATATAAGC
     8281 AATGCTCGTT TAGGGAAC
//

