LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1542..1640)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1655..1674)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1682..1698
                     /note="M13 Reverse priming site"
     gene            1811..2620
                     /note="Kanamycin resistance gene"
     rep_origin      2741..3414
                     /note="pUC origin"
     misc_feature    850..996
                     /note="ef1a cds"
     insert          669..1541
                     /source="Untitled"
                     /type="BP AttB Insert"
     vector          join(1552..3417,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     clone           652..1551
                     /source="Untitled AttB Insert"
                     /type="Expression Clone"
     misc_feature    1520..1521
                     /note="splice acceptor"
     misc_feature    1522..1541
                     /note="exon"
     misc_feature    1012..1112
                     /note="miR-126 pri"
     misc_feature    523..544
                     /note="3560"
     misc_feature    complement(1068..1087)
                     /note="4361"
     source          1..3417
                     /dnas_title="512 pME-mir126new"
ORIGIN      
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCT CGAGTATAAA TTCTCCAACC AAAGCGTTTT TCTTCCTCTT TCTGTTACCT
      721 GGCAAAGGGG AGCAGCAGCT GAGGAGTGAT CTCTCAATCT TGGTGAGTAC TATAGGCTAG
      781 GCCTCTGTGG TACCATTCTA CATGTGTTGA TTTTCTGTAT TTTAGTGAAT TCTGTGATCG
      841 ATAACTTTAA TGCGTATATT GAGGCTTTTC TTCACGGTGG CGGATGTTTT TATGACATTT
      901 TTACGCGGCG GTTTGTTATC CGGCGAGCTT TTGTCAGTTA TTTTATGTTC GGTTTTCTAT
      961 GTGGCTTGTA TGTCTTTCTT AAATGTTTTA TTTTAATAAG TAGTGCGTTT TGAGCCATTT
     1021 TAACTGCTTC ACAGTCCATT ATTACTTTTG GTACGCGCTA GGCCAGACTC AAACTCGTAC
     1081 CGTGAGTAAT AATGCACTGT GGCAGTGGGT TTAAAGTTGA CTTTGTTGTC TCTTTATATT
     1141 TCTGCTGTTT CCGTTATTTT GTGTGTGAAA TGAGCACCGG GAAAAGAACA AAGGCACAAG
     1201 CGGTTTGAAG TCACGGGTCC GTTAAAAGCC TATCACACAT TCCCGCATCC AGCAACTGTT
     1261 ACAAGTGGCC GGAGAGCCAG ATGTCTGCCT CAGTTTGGAT TCCGTTCAGA CATTTCTGTT
     1321 TTTTTTAATC ATCCTGTAAC TTAACACCGT CTTACTCTAA AGAAACACGT GTCGCAGCTG
     1381 CCTTATGGTG AACTAGGCCG GTTCAAACTC TGGGCCATCT TGTCACTTGT GTTACGAAAT
     1441 CACCGCTCAA AAGTTTTAAT AATGAGAAAT ATGTATTTTT GTGGTCCATC ATTAACTTTC
     1501 ACATTTTATC TTGGTCCGCA GAAACTTAGT CGACGGTATC GACCCAGCTT TCTTGTACAA
     1561 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG
     1621 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT
     1681 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC
     1741 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC
     1801 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT
     1861 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC
     1921 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG
     1981 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT
     2041 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC
     2101 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA
     2161 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG
     2221 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG
     2281 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG
     2341 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT
     2401 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG
     2461 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT
     2521 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA
     2581 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT
     2641 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC
     2701 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
     2761 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
     2821 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
     2881 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA
     2941 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
     3001 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
     3061 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
     3121 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
     3181 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
     3241 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
     3301 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
     3361 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT
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