LOCUS       K560-mCh-DHFR           8902 bp    DNA     circular     25-JUN-2015
FEATURES             Location/Qualifiers
     misc_feature    350..949
                     /dnas_title="Pur"
                     /vntifkey="21"
                     /label=Pur
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    312..345
                     /dnas_title="Lox2271"
                     /vntifkey="21"
                     /label=Lox2271
     misc_feature    1..311
                     /dnas_title="TKpA"
                     /vntifkey="21"
                     /label=TKpA
     misc_feature    complement(669..688)
                     /dnas_title="EMO926 (pur_seqdn1)"
                     /vntifkey="21"
                     /label=EMO926 (pur_seqdn1)
     misc_feature    1097..1116
                     /dnas_title="bGH_3'UTR_seq2"
                     /vntifkey="21"
                     /label=bGH_3'UTR_seq2
     misc_feature    1091..1285
                     /dnas_title="bGH_3'UTR"
                     /vntifkey="21"
                     /label=bGH_3'UTR
     misc_feature    1181..1186
                     /dnas_title="pA"
                     /vntifkey="21"
                     /label=pA
     misc_feature    1285..3000
                     /dnas_title="CAG promoter"
                     /vntifkey="21"
                     /label=CAG promoter
     primer_bind     1996..2015
                     /dnas_title="EMO674"
                     /vntifkey="28"
                     /label=EMO674
     primer_bind     complement(2086..2105)
                     /dnas_title="EMO502"
                     /vntifkey="28"
                     /label=EMO502
     misc_feature    5959..6507
                     /dnas_title="globin 3'UTR"
                     /vntifkey="21"
                     /label=globin 3'UTR
     misc_feature    join(5965..5970,6246..6246,6272..6272)
                     /dnas_title="insert detected by sequencing CAG_miR_3U"
                     /note="insert detected by sequencing CAG_miR_3U"
     misc_feature    6530..6563
                     /dnas_title="LoxP"
                     /vntifkey="21"
                     /label=LoxP
     misc_feature    7157..7690
                     /dnas_title="ORI"
                     /vntifkey="21"
                     /label=ORI
     misc_feature    complement(7916..8776)
                     /dnas_title="Bla (AmpR)"
                     /vntifkey="21"
                     /label=Bla (AmpR)
     misc_feature    complement(8777..8875)
                     /dnas_title="Bla promoter"
                     /vntifkey="21"
                     /label=Bla promoter
     misc_feature    4711..5404
                     /note="mCherry"
     misc_feature    5437..5910
                     /note="eDHFR"
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQF
                     LPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
                     /note="residues 2-159"
     misc_feature    5911..5939
                     /note="myc"
                     /translation="EQKLISEEDL"
     insert          3002..4701
                     /source="Kif5b 560 PCR"
                     /type="Custom cloned insert"
     misc_feature    3022..3024
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-acetylalanine; propagated from
                     UniProtKB/Swiss-Prot (P33176.1); acetylation site"
     misc_feature    3034..3993
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /note="Kinesin motor domain, kinesin heavy chain (KHC) or
                     KIF5-like subgroup. Members of this group have been
                     associated with organelle transport. This catalytic
                     (head) domain has ATPase activity and belongs to the
                     larger group of P-loop NTPases. Kinesins are...; Region:
                     KISc_KHC_KIF5; cd01369"
                     /db_xref="CDD:238665"
     misc_feature    3040..4017
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /note="Kinesin-like protein [Cytoskeleton]; Region: KIP1;
                     COG5059"
                     /db_xref="CDD:227392"
     misc_feature    3019..4698
                     /note="K560"
     misc_feature    join(3064..3066,3271..3273,3280..3282,3286..3297,
                     3709..3711)
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:238665"
     exon            3145..3232
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            3233..3306
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            3307..3411
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            3412..3460
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            3461..3516
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            3019..3144
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     CDS             3019..4698
                     /dnas_title="kinesin-1 heavy chain"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /note="kinesin 1 (110-120kD); kinesin heavy chain;
                     ubiquitous kinesin heavy chain; conventional kinesin
                     heavy chain; epididymis secretory protein Li 61"
                     /codon_start=1
                     /product="kinesin-1 heavy chain"
                     /protein_id="NP_004512.1"
                     /db_xref="GI:4758648"
                     /db_xref="CCDS:CCDS7171.1"
                     /db_xref="GeneID:3799"
                     /db_xref="HGNC:HGNC:6324"
                     /db_xref="HPRD:07214"
                     /db_xref="MIM:602809"
                     /translation="MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASK
                     PYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDP
                     EGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKN
                     RVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQ
                     TEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVP
                     YRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVE
                     LTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTV
                     DKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLK
                     TQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQE
                     VEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIG
                     IAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEE
                     NEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHE
                     MEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKM
                     MLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHN
                     LRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADL
                     RCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARR
                     GHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV"
     exon            3517..3604
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    join(3850..3852,3859..3861,3868..3870)
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /note="microtubule interaction site [polypeptide
                     binding]; other site"
                     /db_xref="CDD:238665"
     exon            3605..3729
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            3730..3834
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     STS             3739..3894
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /standard_name="RH93349"
                     /db_xref="UniSTS:88977"
     exon            3835..3980
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            3981..4129
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            4130..4323
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            4324..4392
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     exon            4393..4599
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    4378..4698
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /note="Peptidase Gluzincin family (thermolysin-like
                     proteinases, TLPs) includes peptidases M1, M2, M3, M4,
                     M13, M32 and M36 (fungalysins); Region: GluZincin;
                     cl14813"
                     /db_xref="CDD:265459"
     exon            4600..4698
                     /dnas_title="KIF5B"
                     /gene="KIF5B"
                     /gene_synonym="HEL-S-61; KINH; KNS; KNS1; UKHC"
                     /inference="alignment:Splign:1.39.8"
     source          1..8902
                     /dnas_title="K560-mCh-DHFR"
ORIGIN      
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta
      361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc
     1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga
     1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     3001 ctcgaccacc GGCGCGCCat ggcggacctg gccgagtgca acatcaaagt gatgtgtcgc
     3061 ttcagacctc tcaacgagtc tgaagtgaac cgcggcgaca agtacatcgc caagtttcag
     3121 ggagaagaca cggtcgtgat cgcgtccaag ccttatgcat ttgatcgggt gttccagtca
     3181 agcacatctc aagagcaagt gtataatgac tgtgcaaaga agattgttaa agatgtactt
     3241 gaaggatata atggaacaat atttgcatat ggacaaacat cctctgggaa gacacacaca
     3301 atggagggta aacttcatga tccagaaggc atgggaatta ttccaagaat agtgcaagat
     3361 atttttaatt atatttactc catggatgaa aatttggaat ttcatattaa ggtttcatat
     3421 tttgaaatat atttggataa gataagggac ctgttagatg tttcaaagac caacctttca
     3481 gttcatgaag acaaaaaccg agttccctat gtaaaggggt gcacagagcg ttttgtatgt
     3541 agtccagatg aagttatgga taccatagat gaaggaaaat ccaacagaca tgtagcagtt
     3601 acaaatatga atgaacatag ctctaggagt cacagtatat ttcttattaa tgtcaaacaa
     3661 gagaacacac aaacggaaca aaagctgagt ggaaaacttt atctggttga tttagctggt
     3721 agtgaaaagg ttagtaaaac tggagctgaa ggtgctgtgc tggatgaagc taaaaacatc
     3781 aacaagtcac tttctgctct tggaaatgtt atttctgctt tggctgaggg tagtacatat
     3841 gttccatatc gagatagtaa aatgacaaga atccttcaag attcattagg tggcaactgt
     3901 agaaccacta ttgtaatttg ctgctctcca tcatcataca atgagtctga aacaaaatct
     3961 acactcttat ttggccaaag ggccaaaaca attaagaaca cagtttgtgt caatgtggag
     4021 ttaactgcag aacagtggaa aaagaagtat gaaaaagaaa aagaaaaaaa taagatcctg
     4081 cggaacacta ttcagtggct tgaaaatgag ctcaacagat ggcgtaatgg ggagacggtg
     4141 cctattgatg aacagtttga caaagagaaa gccaacttgg aagctttcac agtggataaa
     4201 gatattactc ttaccaatga taaaccagca accgcaattg gagttatagg aaattttact
     4261 gatgctgaaa gaagaaagtg tgaagaagaa attgctaaat tatacaaaca gcttgatgac
     4321 aaggatgaag aaattaacca gcaaagtcaa ctggtagaga aactgaagac gcaaatgttg
     4381 gatcaggagg agcttttggc atctaccaga agggatcaag acaatatgca agctgagctg
     4441 aatcgccttc aagcagaaaa tgatgcctct aaagaagaag tgaaagaagt tttacaggcc
     4501 ctagaagaac ttgctgtcaa ttatgatcag aagtctcagg aagttgaaga caaaactaag
     4561 gaatatgaat tgcttagtga tgaattgaat cagaaatcgg caactttagc gagtatagat
     4621 gctgagcttc agaaacttaa ggaaatgacc aaccaccaga aaaaacgagc agctgagatg
     4681 atggcatctt tactaaaagg ACCGGTcacc ATGGTGAGCA AGGGCGAGGA GGATAACATG
     4741 GCCATCATCA AGGAGTTCAT GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC
     4801 GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG
     4861 CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC
     4921 ATGTACGGCT CCAAGGCCTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG
     4981 TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG
     5041 ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC
     5101 GGCACCAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC
     5161 TCCTCCGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG
     5221 AAGCTGAAGG ACGGCGGCCA CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG
     5281 CCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC ctcccacaac
     5341 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     5401 atggacgagc tgtataaGGC CGGCCTGGAG TCTAGAATCA GTCTGATTGC GGCGTTAGCG
     5461 GTAGATCGCG TTATCGGCAT GGAAAACGCC ATGCCGTGGA ACCTGCCTGC CGATCTCGCC
     5521 TGGTTTAAAC GCAACACCTT AAATAAACCC GTGATTATGG GCCGCCATAC CTGGGAATCA
     5581 ATCGGTCGTC CGTTGCCAGG ACGCAAAAAT ATTATCCTCA GCAGTCAACC GGGTACGGAC
     5641 GATCGCGTAA CGTGGGTGAA GTCGGTGGAT GAAGCCATCG CGGCGTGTGG TGACGTACCA
     5701 GAAATCATGG TGATTGGCGG CGGTCGCGTT TATGAACAGT TCTTGCCAAA AGCGCAAAAA
     5761 CTGTATCTGA CGCATATCGA CGCAGAAGTG GAAGGCGACA CCCATTTCCC GGATTACGAG
     5821 CCGGATGACT GGGAATCGGT ATTCAGCGAA TTCCACGATG CTGATGCGCA GAACTCTCAC
     5881 AGCTATTGCT TTGAGATTCT GGAGCGGCGG GAACAAAAAC TCATCTCAGA AGAGGATCTt
     5941 taaTTAAGCG CTCGAGgcgg ccgcAATTCA ctcctcaggt gcaggctgcc tatcagaagg
     6001 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     6061 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     6121 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     6181 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     6241 atgccCatat gctggctgcc atgaacaaag gTtggctata aagaggtcat cagtatatga
     6301 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     6361 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     6421 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     6481 tcttatgaag atccctcgac ctgagcgtcg agctagcata tggatccata taacttcgta
     6541 taatgtatgc tatacgaagt tattacgata ccgtcaactt gtttattgca gcttataatg
     6601 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaggt acccagcttt tgttcccttt
     6721 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6781 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6841 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6901 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6961 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7021 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7081 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7141 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7201 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7261 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7321 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7381 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7441 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7501 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7561 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7621 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7681 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7741 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7801 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7861 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8161 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8341 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8461 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8581 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8641 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8761 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8821 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8881 gcacatttcc ccgaaaagtg cc
//

