LOCUS       Exported File          19400 bp ds-DNA    circular SYN 22-SEP-2013
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDNA-ROSA26-EF1ahCas9IRESneo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19400)
  AUTHORS   Kosuke Yusa
  TITLE     Direct Submission
  JOURNAL   Exported 22 Sep 2013 from SnapGene Viewer 2.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19400
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     600..616
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    681..4051
                     /note="Right arm"
     misc_feature    4053..4174
                     /note="Ad-SA"
     protein_bind    4181..4214
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    4215..4239
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4277..5876
                     /note="hEF1a promoter"
     intron          5664..5867
                     /note="EF-1-alpha intron A"
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     CDS             5895..10034
                     /codon_start=1
                     /note="hCas9"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDSRADPKKKRKV"
     CDS             10011..10031
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     misc_feature    10081..10666
                     /note="IRES2"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
     CDS             10667..11470
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    11508..11732
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(11777..11801)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    11811..12035
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    12048..15620
                     /note="Left arm"
     CDS             15923..16576
                     /codon_start=1
                     /note="DT-A"
                     /translation="MDPDDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQG
                     NYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIK
                     KELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVE
                     LEINFETRGKRGQDAMYEYMAQACAGNRVRRSLCEGTLLLWCDIIGQTTYRDLKL"
     primer_bind     complement(17251..17267)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(18412..19272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 ttaaacggcg cgccggtacc ttaattaaac cagcaatgtg gatataagca ttaagtttta
      721 gttttaaaaa ggtcaatttt ccaaacattc agcaatcata tttaaattta cagctaggaa
      781 caagagcctt gggtcatgtc ctaccaaaga acataactca atattctaca catgacaatc
      841 tgaataacct taaagcctct aatcccataa caggccacaa attttggaca gagaactaat
      901 gatcctcctg agaaaactgg aagaaatcca gggaaaagaa attcctgtgt cctccaaact
      961 cagaaatctc taattatgtc agtattctct gctttagtcc taggtcagat tgcacacatc
     1021 taaaataacc tcttaaagtt ttcctcctag cgacctaaac cattattaat atcaaattaa
     1081 ccatcaaaac actttcctct caatatgctg cacacaaacc tcctcctgga acctcctcca
     1141 tctggatcct ccccaatcaa aagtataggt atttaacata taagcaagga agtaatgtaa
     1201 acatgacctt ggtcacaaat atgtcatcta aaaacaattt agtcaaggta tggaggaaat
     1261 tcgagaacct gaatcttttt aagtattttg agcacaggaa caattggcaa aaggaatcca
     1321 ggtatagaca aaacccagag cccagagctc tgggcgaaaa atgagttgct ggtgaagacg
     1381 ttacacaagt aacatgagaa agcagaaaat gcaggtcatc cacgcacccc tgacccaggc
     1441 cagcagggcg ggctgcagca tcagtacaca ggagaaagat ccttattcct aagaatgaga
     1501 aaggcaaagg cgcccgatag aataaattag catagaaggg gctttcccag gagttaaaac
     1561 tttccttctg agcgattacc tactaaaacc agggcttttg cccactacca tttacctagg
     1621 atcttggctt gcacggattc ataggggcat atccctcccc ctcttcttta gagtcgttct
     1681 taaaagatcg ctctccacgc cctaggcagg gaaaacgaca aaatctggct caattccagg
     1741 ctagaaccct acaaattcaa cagggatatc gcaaggatac tggggcatac gccacaggga
     1801 gtccaagaat gtgaggtggg ggtggcgaag gtaatgtctt tggtgtggga aaagcagcag
     1861 ccatctgaga taggaactgg aaaaccagag gagaggcgtt caggaagatt atggagggga
     1921 ggactgggcc cccacgagcg accagagttg tcacaaggcc gcaagaacag gggaggtggg
     1981 gggctcaggg acagaaaaaa aagtatgtgt attttgagag cagggttggg aggcctctcc
     2041 tgaaaagggt ataaacgtgg agtaggcaat acccaggcaa aaaggggaga ccagagtagg
     2101 gggaggggaa gagtcctgac ccagggaaga cattaaaaag gtagtggggt cgactagatg
     2161 aaggagagcc tttctctctg ggcaagagcg gtgcaatggt gtgtaaaggt agctgagaag
     2221 acgaaaaggg caagcatctt cctgctacca ggctggggag gcccaggccc acgaccccga
     2281 ggagagggaa cgcagggaga ctgaggtgac ccttctttcc cccggggccc ggtcgtgtgg
     2341 ttcggtgtct cttttctgtt ggacccttac cttgacccag gcgctgccgg ggcctgggcc
     2401 cgggctgcgg cgcacggcac tcccgggagg cagcgagact cgagttaggc ccaacgcggc
     2461 gccacggcgt ttcctggccg ggaatggccc gtacccgtga ggtgggggtg gggggcagaa
     2521 aaggcggagc gagcccgagg cggggagggg gagggccagg ggcggagggg gccggcacta
     2581 ctgtgttggc ggactggcgg gactagggct gcgtgagtct ctgagcgcag gcgggcggcg
     2641 gccgcccctc ccccggcggc ggcagcggcg gcagcggcgg cagctcactc agcccgctgc
     2701 ccgagcggaa acgccactga ccgcacgggg attcccagtg ccggcgccag gggcacgcgg
     2761 gacacgcccc ctcccgccgc gccattggcc tctccgccca ccgccccaca cttattggcc
     2821 ggtgcgccgc caatcagcgg aggctgccgg ggccgcctaa agaagaggct gtgctttggg
     2881 gctccggctc ctcagagagc ctcggctagg taggggatcg ggactctggc gggagggcgg
     2941 cttggtgcgt ttgcggggat gggcggccgc ggcaggccct ccgagcgtgg tggagccgtt
     3001 ctgtgagaca gccgggtacg agtcgtgacg ctggaagggg caagcgggtg gtgggcagga
     3061 atgcggtccg ccctgcagca accggagggg gagggagaag ggagcggaaa agtctccacc
     3121 ggacgcggcc atggctcggg gggggggggg cagcggagga gcgcttccgg ccgacgtctc
     3181 gtcgctgatt ggcttctttt cctcccgccg tgtgtgaaaa cacaaatggc gtgttttggt
     3241 tggcgtaagg cgcctgtcag ttaacggcag ccggagtgcg cagccgccgg cagcctcgct
     3301 ctgcccactg ggtggggcgg gaggtaggtg gggtgaggcg agctggacgt gcgggcgcgg
     3361 tcggcctctg gcggggcggg ggaggggagg gagggtcagc gaaagtagct cgcgcgcgag
     3421 cggccgccca ccctcccctt cctctggggg agtcgtttta cccgccgccg gccgggcctc
     3481 gtcgtctgat tggctctcgg ggcccagaaa actggccctt gccattggct cgtgttcgtg
     3541 caagttgagt ccatccgccg gccagcgggg gcggcgagga ggcgctccca ggttccggcc
     3601 ctcccctcgg ccccgcgccg cagagtctgg ccgcgcgccc ctgcgcaacg tggcaggaag
     3661 cgcgcgctgg gggcggggac gggcagtagg gctgagcggc tgcggggcgg gtgcaagcac
     3721 gtttccgact tgagttgcct caagaggggc gtgctgagcc agacctccat cgcgcactcc
     3781 ggggagtgga gggaaggagc gagggctcag ttgggctgtt ttggaggcag gaagcacttg
     3841 ctctcccaaa gtcgctctga gttgttatca gtaagggagc tgcagtggag taggcgggga
     3901 gaaggccgca cccttctccg gaggggggag gggagtgttg caataccttt ctgggagttc
     3961 tctgctgcct cctggcttct gaggaccgcc ctgggcctgg gagaatccct tccccctctt
     4021 ccctcgtgat ctgcaactcc agtcttacta gtatctgtag ggcgcagtag tccagggttt
     4081 ccttgatgat gtcatactta tcctgtccct tttttttcca cagctcgcgg ttgaggacaa
     4141 actcttcgcg gtctttccag tggggatcga cggtggatcc ataacttcgt atagcataca
     4201 ttatacgaag ttatacaagt ttgtacaaaa aagcaggctg gcgccggaac caattcagtc
     4261 gactggatcc acgcgtgagg ggcgcggaag ccggggcgga ggaggagcca accccgggct
     4321 gggctgagac ccgcagagga agacgctcta gggatttgtc ccggactagc gagatggcaa
     4381 ggctgaggac gggaggctga ttgagaggcg aaggtacacc ctaatctcaa tacaaccttt
     4441 ggagctaagc cagcaatggt agagggaaga ttctgcacgt cccttccagg cggcctcccc
     4501 gtcaccaccc cccccaaccc gccccgaccg gagctgagag taattcatac aaaaggactc
     4561 gcccctgcct tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga
     4621 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag
     4681 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4741 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     4801 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4861 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4921 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     4981 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     5041 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     5101 agttgaggcc tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     5161 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     5221 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatcgatctg cacactggta
     5281 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     5341 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     5401 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     5461 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     5521 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     5581 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     5641 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     5701 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     5761 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     5821 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgggaag
     5881 cttggtcgac caccatggac aagaagtact ccattgggct cgatatcggc acaaacagcg
     5941 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
     6001 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
     6061 gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
     6121 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
     6181 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
     6241 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
     6301 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
     6361 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
     6421 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
     6481 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
     6541 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
     6601 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
     6661 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
     6721 acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc
     6781 tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc
     6841 cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga
     6901 aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta
     6961 aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta
     7021 ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag
     7081 aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc
     7141 tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata
     7201 acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg
     7261 cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct
     7321 ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga
     7381 ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg
     7441 agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa
     7501 agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga
     7561 accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg
     7621 actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg
     7681 atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc
     7741 ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct
     7801 tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat
     7861 atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg
     7921 gaaagacaat cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt
     7981 tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc
     8041 agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg
     8101 gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc
     8161 ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga
     8221 acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc
     8281 ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc
     8341 tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact
     8401 acgacgtgga tcatatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag
     8461 tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg
     8521 tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga
     8581 agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct
     8641 tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg
     8701 attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta
     8761 ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga
     8821 gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg
     8881 cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt
     8941 acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt
     9001 acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag
     9061 agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca
     9121 agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta
     9181 aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca
     9241 gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt
     9301 ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa
     9361 aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa
     9421 aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca
     9481 ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta
     9541 gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct
     9601 tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc
     9661 agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat
     9721 tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata
     9781 agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga
     9841 ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt
     9901 acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct
     9961 atgaaacaag aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga
    10021 agaggaaggt gtgaaagggt tcgatcccta ccggtatcga tgtgcggccg ccgccccccc
    10081 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
    10141 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
    10201 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
    10261 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
    10321 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
    10381 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
    10441 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
    10501 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
    10561 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
    10621 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg gatcggccat
    10681 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
    10741 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
    10801 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
    10861 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
    10921 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
    10981 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
    11041 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
    11101 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
    11161 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
    11221 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
    11281 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
    11341 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
    11401 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
    11461 gttcttctga cgtacgcgat gatctagagc tcgctgatca gcctcgactg tgccttctag
    11521 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
    11581 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
    11641 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
    11701 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa cctgcaacta
    11761 gtctcgagat atctagaccc agctttcttg tacaaagtgg tgaattccga ctgtgccttc
    11821 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
    11881 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
    11941 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
    12001 tagcaggcat gctggggatg cggtgggctc tatgggtcga cctcgagaga tgggcgggag
    12061 tcttctgggc aggcttaaag gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga
    12121 acaggtgtaa aattggaggg acaagacttc ccacagattt tcggttttgt cgggaagttt
    12181 tttaataggg gcaaataagg aaaatgggag gataggtagt catctggggt tttatgcagc
    12241 aaaactacag gttattattg cttgtgatcc gcctcggagt attttccatc gaggtagatt
    12301 aaagacatgc tcacccgagt tttatactct cctgcttgag atccttacta cagtatgaaa
    12361 ttacagtgtc gcgagttaga ctatgtaagc agaattttaa tcatttttaa agagcccagt
    12421 acttcatatc catttctccc gctccttctg cagccttatc aaaaggtatt ttagaacact
    12481 cattttagcc ccattttcat ttattatact ggcttatcca acccctagac agagcattgg
    12541 cattttccct ttcctgatct tagaagtctg atgactcatg aaaccagaca gattagttac
    12601 atacaccaca aatcgaggct gtagctgggg cctcaacact gcagttcttt tataactcct
    12661 tagtacactt tttgttgatc ctttgccttg atccttaatt ttcagtgtct atcacctctc
    12721 ccgtcaggtg gtgttccaca tttgggccta ttctcagtcc agggagtttt acaacaatag
    12781 atgtattgag aatccaacct aaagcttaac tttccactcc catgaatgcc tctctccttt
    12841 ttctccattt ataaactgag ctattaacca ttaatggttt ccaggtggat gtctcctccc
    12901 ccaatattac ctgatgtatc ttacatattg ccaggctgat attttaagac attaaaaggt
    12961 atatttcatt attgagccac atggtattga ttactgctta ctaaaatttt gtcattgtac
    13021 acatctgtaa aaggtggttc cttttggaat gcaaagttca ggtgtttgtt gtctttcctg
    13081 acctaaggtc ttgtgagctt gtattttttc tatttaagca gtgctttctc ttggactggc
    13141 ttgactcatg gcattctaca cgttattgct ggtctaaatg tgattttgcc aagcttcttc
    13201 aggacctata attttgcttg acttgtagcc aaacacaagt aaaatgatta agcaacaaat
    13261 gtatttgtga agcttggttt ttaggttgtt gtgttgtgtg tgcttgtgct ctataataat
    13321 actatccagg ggctggagag gtggctcgga gttcaagagc acagactgct cttccagaag
    13381 tcctgagttc aattcccagc aaccacatgg tggctcacaa ccatctgtaa tgggatctga
    13441 tgccctcttc tggtgtgtct gaagaccaca agtgtattca cattaaataa ataaatcctc
    13501 cttcttcttc tttttttttt ttttaaagag aatactgtct ccagtagaat ttactgaagt
    13561 aatgaaatac tttgtgtttg ttccaatatg gtagccaata atcaaattac tctttaagca
    13621 ctggaaatgt taccaaggaa ctaattttta tttgaagtgt aactgtggac agaggagcca
    13681 taactgcaga cttgtgggat acagaagacc aatgcagact ttaatgtctt ttctcttaca
    13741 ctaagcaata aagaaataaa aattgaactt ctagtatcct atttgtttaa actgctagct
    13801 ttacttaact tttgtgcttc atctatacaa agctgaaagc taagtctgca gccattacta
    13861 aacatgaaag caagtaatga taattttgga tttcaaaaat gtagggccag agtttagcca
    13921 gccagtggtg gtgcttgcct ttatgccttt aatcccagca ctctggaggc agagacaggc
    13981 agatctctga gtttgagccc agcctggtct acacatcaag ttctatctag gatagccagg
    14041 aatacacaca gaaaccctgt tggggagggg ggctctgaga tttcataaaa ttataattga
    14101 agcattccct aatgagccac tatggatgtg gctaaatccg tctacctttc tgatgagatt
    14161 tgggtattat tttttctgtc tctgctgttg gttgggtctt ttgacactgt gggctttctt
    14221 taaagcctcc ttcctgccat gtggtctctt gtttgctact aacttcccat ggcttaaatg
    14281 gcatggcttt ttgccttcta agggcagctg ctgagatttg cagcctgatt tccagggtgg
    14341 ggttgggaaa tctttcaaac actaaaattg tcctttaatt ttttttttaa aaaatgggtt
    14401 atataataaa cctcataaaa tagttatgag gagtgaggtg gactaatatt aaatgagtcc
    14461 ctcccctata aaagagctat taaggctttt tgtcttatac ttaacttttt ttttaaatgt
    14521 ggtatcttta gaaccaaggg tcttagagtt ttagtataca gaaactgttg catcgcttaa
    14581 tcagattttc tagtttcaaa tccagagaat ccaaattctt cacagccaaa gtcaaattaa
    14641 gaatttctga cttttaatgt taatttgctt actgtgaata taaaaatgat agcttttcct
    14701 gaggcagggt ctcactatgt atctctgcct gatctgcaac aagatatgta gactaaagtt
    14761 ctgcctgctt ttgtctcctg aatactaagg ttaaaatgta gtaatacttt tggaacttgc
    14821 aggtcagatt cttttatagg ggacacacta agggagcttg ggtgatagtt ggtaaaatgt
    14881 gtttcaagtg atgaaaactt gaattattat caccgcaacc tactttttaa aaaaaaaagc
    14941 caggcctgtt agagcatgct taagggatcc ctaggacttg ctgagcacac aagagtagtt
    15001 acttggcagg ctcctggtga gagcatattt caaaaaacaa ggcagacaac caagaaacta
    15061 cagttaaggt tacctgtctt taaaccatct gcatatacac agggatatta aaatattcca
    15121 aataatattt cattcaagtt ttcccccatc aaattgggac atggatttct ccggtgaata
    15181 ggcagagttg gaaactaaac aaatgttggt tttgtgattt gtgaaattgt tttcaagtga
    15241 tagttaaagc ccatgagata cagaacaaag ctgctatttc gaggtctctt ggtttatact
    15301 cagaagcact tctttgggtt tccctgcact atcctgatca tgtgctaggc ctaccttagg
    15361 ctgattgttg ttcaaataaa cttaagtttc ctgtcaggtg atgtcatatg atttcatata
    15421 tcaaggcaaa acatgttata tatgttaaac atttgtactt aatgtgaaag ttaggtcttt
    15481 gtgggtttga tttttaattt tcaaaacctg agctaaataa gtcattttta catgtcttac
    15541 atttggtgga attgtataat tgtggtttgc aggcaagact ctctgaccta gtaaccctac
    15601 ctatagagca ctttgctggg actagtggat cctctagagt cgagcagtgt ggttttcaag
    15661 aggaagcaaa aagcctctcc acccaggcct ggaatgtttc cacccaatgt cgagcagtgt
    15721 ggttttgcaa gaggaagcaa aaagcctctc cacccaggcc tggaatgttt ccacccaatg
    15781 tcgagcaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg
    15841 ggcggcgcgg tcccaggtcc acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg
    15901 agcgaccctg caggtcctcg ccatggatcc tgatgatgtt gttgattctt ctaaatcttt
    15961 tgtgatggaa aacttttctt cgtaccacgg gactaaacct ggttatgtag attccattca
    16021 aaaaggtata caaaagccaa aatctggtac acaaggaaat tatgacgatg attggaaagg
    16081 gttttatagt accgacaata aatacgacgc tgcgggatac tctgtagata atgaaaaccc
    16141 gctctctgga aaagctggag gcgtggtcaa agtgacgtat ccaggactga cgaaggttct
    16201 cgcactaaaa gtggataatg ccgaaactat taagaaagag ttaggtttaa gtctcactga
    16261 accgttgatg gagcaagtcg gaacggaaga gtttatcaaa aggttcggtg atggtgcttc
    16321 gcgtgtagtg ctcagccttc ccttcgctga ggggagttct agcgttgaat atattaataa
    16381 ctgggaacag gcgaaagcgt taagcgtaga acttgagatt aattttgaaa cccgtggaaa
    16441 acgtggccaa gatgcgatgt atgagtatat ggctcaagcc tgtgcaggaa atcgtgtcag
    16501 gcgatctctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
    16561 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
    16621 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
    16681 ggtggaatgc agatccacta gagtcgaggg atgcagaaat tgatgatcta ttaaacaata
    16741 aagatgtcca ctaaaatgga agtttttcct gtcatacttt gttaagaagg gtgagaacag
    16801 agtacctaca ttttgaatgg aaggattgga gctacggggg tgggggtggg gtgggattag
    16861 ataaatgcct gctctttact gaaggctctt tactattgct ttatgataat gtttcatagt
    16921 tggatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca
    16981 tgatctatag atctatagat ctctcgtggg atcattgttt ttctcttgat tcccactttg
    17041 tggttctaag tactgtggtt tccaaatgtg tcagtttcat agcctgaaga acgagatcag
    17101 cagcctctgt tccacataca cttcattctc agtattgttt tgccaagttc taattccatc
    17161 agaagctggt cgatcgactc tagctagatg cggcgtaccc agcttttgtt ccctttagtg
    17221 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    17281 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    17341 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    17401 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    17461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    17521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    17581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    17641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    17701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    17761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    17821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    17881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    17941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    18001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    18061 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    18121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    18181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    18241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    18301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    18361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    18421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    18481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    18541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    18601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    18661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    18721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    18781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    18841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    18901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    18961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    19021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    19081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    19141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    19201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    19261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    19321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    19381 atttccccga aaagtgccac
//
