LOCUS       pRT802                  5146 bp    DNA     circular SYN 05-MAR-2018
DEFINITION  Ligation of kan into Fragment 2.
ACCESSION   pRT802with
VERSION
KEYWORDS    .
SOURCE      Unknown
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational
            Software SECNOTES|600bp deletion introduced to the right ot the
            hindiii side of the hindiii -saci fragment coming from pNRT4.
FEATURES             Location/Qualifiers
     CDS             1259..1287
                     /gene="aac3(IV)'"
     CDS             complement(1469..2393)
                     /gene="aphII"
     misc_feature    2850..2961
                     /gene="oriT"
     misc_feature    3147..3197
     CDS             3252..4681
                     /gene="int"
ORIGIN      
        1 gatccgcggc cgcgcgcgat atcgaattcg taatcatgtc atagctgttt cctgtgtgaa
       61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      841 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg
     1141 ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca
     1201 acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt
     1261 gcaatacgaa tggcgaaaag ccgagctcgg tacccggggt gggcgaagaa ctccagcatg
     1321 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     1381 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     1441 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     1501 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     1561 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     1621 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     1681 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
     1741 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     1801 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     1861 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     1921 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     1981 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     2041 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     2101 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     2161 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     2221 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     2281 ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     2341 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     2401 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag
     2461 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     2521 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
     2581 agcccttgcg ccctgagtgc ttgcggcagc gtcgccattg atgcgggcca gctcgcggac
     2641 gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc
     2701 cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag
     2761 gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg
     2821 cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt
     2881 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
     2941 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
     3001 cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat
     3061 aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgacctgcag gcatgctggc
     3121 gccggacggg gcttcagacg tttcgggtgc tgggttgttg tctctggaca gtgatccatg
     3181 ggaaactact cagcaccacc aatgttccca aaagaaagcg caggtcagcg cccatgagcc
     3241 aagatctagg catgtcgccc ttcatcgctc ccgacgtccc tgagcacctt ctagacactg
     3301 ttcgcgtctt cctgtacgcg cgtcagtcta agggccggtc cgacggctca gacgtgtcga
     3361 ccgaagcaca gctagcggcc ggtcgtgcgt tggtcgcgtc tcgcaacgcc caggggggtg
     3421 cgcgctgggt cgtggcaggt gagttcgtgg acgtcgggcg ctccggctgg gacccgaacg
     3481 tgacccgtgc cgacttcgag cgcatgatgg gcgaagtccg cgccggcgaa ggtgacgttg
     3541 tcgttgtgaa tgagctttcc cggctcactc gcaagggcgc ccatgacgcg ctcgaaatcg
     3601 acaacgaatt gaagaagcac ggcgtgcgct tcatgtcggt tcttgagccg ttccttgaca
     3661 cgtctacccc tatcggcgtc gccattttcg cgctgatcgc tgcccttgcg aaacaggaca
     3721 gtgacctgaa ggcggagcgc ctgaagggtg cgaaagacga gattgccgcg ctgggtggcg
     3781 ttcactcgtc ttccgccccg ttcggaatgc gcgccgtgcg caagaaggtc gataatctcg
     3841 tgatctccgt tcttgagccg gacgaagaca acccggatca cgtcgagcta gttgagcgca
     3901 tggcgaaaat gtcgttcgaa ggcgtgtccg acaacgccat tgcaacgacc ttcgagaagg
     3961 aaaagatccc gtcgcccgga atggctgaga gacgcgccac ggaaaagcgt cttgcgtcca
     4021 tcaaggcacg tcgcctgaac ggcgctgaaa agccgatcat gtggcgcgct caaacggtcc
     4081 gatggattct caaccatccc gcaatcggcg gtttcgcatt cgagcgtgtg aagcacggta
     4141 aggcgcacat caacgtcata cggcgcgacc ccggcggcaa gccgctaacg ccccacacgg
     4201 gcattctcag cggctcgaag tggcttgagc ttcaagagaa gcgttccggg aagaatctca
     4261 gcgaccggaa gcctggggcc gaagtcgaac cgacgcttct gagcgggtgg cgtttcctgg
     4321 ggtgccgaat ctgcggcggc tcaatgggtc agtcccaggg tggccgtaag cgcaacggcg
     4381 accttgccga aggcaattac atgtgcgcca acccgaaggg gcacggcggc ttgtcggtca
     4441 agcgcagcga actggacgaa ttcgttgctt cgaaggtgtg ggcacggctc cgcacagccg
     4501 acatggaaga tgaacacgat caggcatgga ttgccgccgc tgcggagcgc ttcgcccttc
     4561 agcacgacct agcgggggtg gccgatgagc ggcgcgaaca acaggcgcac ctagacaacg
     4621 tgcggcgctc catcaaggac cttcaggcgg accgtaaggc cggtctgtac gtcgggcgtg
     4681 aagagctgga aacgtggcgc tcaacggtgc tgcaataccg gtcctacgaa gcggagtgca
     4741 cgacccgact cgctgagctt gacgagaaga tgaacggcag cacccgcgtt ccgtctgagt
     4801 ggttcagcgg cgaagacccg acggccgaag ggggcatctg ggcaagctgg gacgtgtacg
     4861 agcgtcggga gttcctgagc ttcttccttg actccgtcat ggtcgaccgg gggcgccacc
     4921 ctgagacgaa gaaatacatc cccctgaagg accgtgtgac gctcaagtgg gcggagctgc
     4981 tgaaggagga agacgaagcg agcgaagcca ctgagcggga gcttgcggcg ctgtagcgca
     5041 cagcgggagg ggtcgagccg gcggacggtt cggccccttt tttggccttg aaatcgttag
     5101 ttaggctaac tagtagttcc ttcgtcacca tagcgggcag ggagcg
//
