LOCUS       pED45.xdna    6552 bp      DNA     circular   16.03.15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : oED0165(for).xdna [92  nt] : (EcoRI[5] / BglII
            [101]) to pED40(DS1p33- NLSSP8).xdna [6452  nt] : (BglII[302
            3] / EcoRI[3014])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    269..1380
                     /label=insert from pED40(DS1p33- NLSSP8).xdna [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED40(DS1p33- NLSSP8).xdna [6452  nt] : (BglII[3023] / EcoRI[3014]) \ 
                     2589..172
                     /label=insert from pED40(DS1p33- NLSSP8).xdna [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED40(DS1p33- NLSSP8).xdna [6452  nt] : (BglII[3023] / EcoRI[3014]) \ 
     misc_feature    complement(4925..4939)
                     /label=pED1-6 Ann.Seq [3']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
     misc_feature    4992..5003
                     /label=pED1-6 Ann.Seq [5']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
     misc_feature    5013..5016
                     /label=pED1-6 Ann.Seq [3']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
                     5020..6026
                     /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356  nt] : (BglII[3203] / XbaI[3104]) \ 
     misc_feature    1276..1380
                     /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [3'] [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356  nt] : (BglII[3203] / XbaI[3104]) \ 
     misc_feature    2589..4914
                     /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [3'] [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356  nt] : (BglII[3203] / XbaI[3104]) \ 
     CDS             complement(3671..4330)
                     /label=beta Lactamase
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=dnas_title="Beta Lactamase" - codon_start=True - label=Beta Lactamase - SerialCloner_Color="&h8FBC8F" - SerialCloner_Show="True" - SerialCloner_Protect="False" - SerialCloner_Arrow="True" - note="Copied from " - label=beta Lactamase - translation="MSTFKVL \ 
     promoter        complement(4570..4598)
                     /label=Copied from _Amp prom
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Amp prom - SerialCloner_Color="&h4682B4" - SerialCloner_Show="True" - SerialCloner_Protect="False" - SerialCloner_Arrow="True" - note="Copied from " - complement(6385..6413) \ 
     Promoter        complement(4570..4598)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             5139..5942
                     /label=Ura
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    6203..6509
                     /label=F1_Origin
                     /SerialCloner_Color=&hFFCC66
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     796..812
                     /label=Seq for oED14
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     1175..1204
                     /label=oSP1
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        173..1174
                     /label=pRPS2
                     /SerialCloner_Color=&h80FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1778..1795
                     /label=Seq rev oED15
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    1205..1231
                     /label=Ste7 DS1
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1481..2182
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1316..1361
                     /label=Swi6NLSSP8
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1412..1457
                     /label=Swi6NLSSP8
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaagggg 
       61 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 
      121 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga gctCGTTGCC 
      181 ACGATTGGAA TACCCTCAAA ATTCCCACTT TTACTATGCT TCAATTGACC CTTCGGTATC 
      241 TCAGCATCTA GGTAGCTTTC ATTGAGATTC ATAGTTCCGA TGTACTGATT CTACGTTTGT 
      301 GTACTTTGAT AGACTTTTTT ATTCAATTCG TATTCACTAC TATCTTTGTC TCGTACCGCG 
      361 GTTTTTATCC TTTATTTCAG CCCTACGAGA TAGAACGATG GAGCCTAGTA ATACCATTTT 
      421 ATGTAAGCAA AATAAGAAGA AAGACTAAAA GAATTAGGAT ATGTTGTTAA TCAGGGATAA 
      481 AGGTACGCAT ACTAGCCTTA CTACGTAAAT ATAAGGTTAC GGTTTACGAT CACTATTGCA 
      541 AGATACTATT GTTTAGCATT GGCAAAGACT TGACGGGAAA TCTTTGATTG GGCCCAACCA 
      601 TATTCATTAA AGATCCGGGA TGTGAGCTTG AACTGCTTTT CGACGCGAAG AAGGTTTACA 
      661 CGTATTTTAG TAATTGGCTT ATTCACTAAG GATTCTTAAG GTTTTCTTAA TAGTTTTCTA 
      721 CGTCGGCATG CGATTGTTTG GTTTAGAAGA CTGCTTTCTA AATATGGTTG GGTGTATTTA 
      781 AGCTAGACCC ATACACCCGC TCTATGGGAT TATTTACTTG TTTGAATTTT AAGATTTGTG 
      841 ATAATGGAAC TGGACGCAAA CATTTGATGG AAAACGCATG TCATCATTAA CGAGGTAACG 
      901 TAGGTATCTG TCCTGCCTTA GTATTGCACG CAGCTTCCCA GGACGCCTAG CTATTTTTTC 
      961 ATCTATTCCC CTCTGTAGTA ACGTAAGAGT TTTCAAGTTT TTAATTCAGA CTTTCTCTTC 
     1021 CTTTGTTTCC AATTTCCTTC CTTACTGCTT GATACCTTTT CAATCCCAAA GAAACCGTGT 
     1081 TCTTTATATA TTGTCGATTG AAAGTTACCT ACATCAACTT TCCGTGTTCC ATTCCGACTA 
     1141 TAACAAACAA CCAATAAGCT CAACTAATTA AGTAAagctT ATGTTTCAAC GAAAGACTTT 
     1201 ACAGagaagg aacttgaaag ggctcaatct taacctgcac ccagatgtgg gcaataatgg 
     1261 ccaattgcag gaaaagacaT ctagaCAACA AATGGGTCGT GGTTCGGAAT TTGAAGAACT 
     1321 AGGTTCCCCG CTAAAAAAAC TAAAAATATC ACCAGATACG GCTTCTGGTT TGGTTgaatt 
     1381 cCAACAAATG GGTCGTGGTT CGGAATTTGA AGAACTAGGT TCCCCGCTAA AAAAACTAAA 
     1441 AATATCACCA GATACGGCTT CTGGTTTGGT TagatcTGCT AGCAAGGGCG AGGAGGATAA 
     1501 CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG 
     1561 CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC 
     1621 CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA 
     1681 GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA 
     1741 GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT 
     1801 GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT 
     1861 GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA 
     1921 GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG 
     1981 GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA 
     2041 GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA 
     2101 CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG 
     2161 CGGCATGGAC GAGCTGTACA AGGGATCTTA ACtcgagtca tgtaattagt tatgtcacgc 
     2221 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct 
     2281 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata 
     2341 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa 
     2401 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtacccagct 
     2461 tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc 
     2521 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat aggagccgga agcataaagt 
     2581 gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc 
     2641 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 
     2701 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     2761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     2821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     2881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct cggcccccct gacgagcatc 
     2941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     3001 cgttcccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     3061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 
     3121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     3181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     3241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     3301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     3361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     3421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     3481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     3541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     3601 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     3661 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     3721 catccatagt tgcctgactg cccgtcgtgt agataactac gatacgggag ggcttaccat 
     3781 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     3841 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     3901 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     3961 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     4021 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgaa 
     4081 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     4141 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     4201 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     4261 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     4321 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     4381 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     4441 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     4501 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     4561 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     4621 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 
     4681 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 
     4741 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 
     4801 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 
     4861 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cacgcttttc 
     4921 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt 
     4981 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg 
     5041 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac 
     5101 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg 
     5161 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa 
     5221 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag 
     5281 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt 
     5341 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac 
     5401 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg 
     5461 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag 
     5521 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt 
     5581 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta 
     5641 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca 
     5701 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg 
     5761 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat 
     5821 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg 
     5881 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact 
     5941 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt 
     6001 aattatatca gttattaccc tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 
     6061 ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 
     6121 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 
     6181 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 
     6241 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 
     6301 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 
     6361 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 
     6421 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 
     6481 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg ccattcgcca 
     6541 ttcaggctgc gc
//