LOCUS       pED54(DoubSP9PhosMutE).xdna    6552 bp      DNA     circular   23.07.15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pED52(SP9PhosMutE).xdna [6446  nt] : (NheI[121
            9] / EcoRI[1204]) to oED202(NLSSP9PMutEFor2).xdna [98  nt] :
             (EcoRI[5] / NheI[107])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2417..6552
                     /label=insert from pED40(DS1p33- NLSSP8).xdna [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED40(DS1p33- NLSSP8).xdna [6452  nt] : (BglII[3023] / EcoRI[3014]) \ 
     misc_feature    complement(4753..4767)
                     /label=pED1-6 Ann.Seq [3']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
     misc_feature    4820..4831
                     /label=pED1-6 Ann.Seq [5']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
     misc_feature    4841..4844
                     /label=pED1-6 Ann.Seq [3']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
                     4848..5854
                     /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356  nt] : (BglII[3203] / XbaI[3104]) \ 
     misc_feature    2417..4742
                     /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [3'] [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356  nt] : (BglII[3203] / XbaI[3104]) \ 
     CDS             complement(3499..4158)
                     /label=beta Lactamase
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=dnas_title="Beta Lactamase" - codon_start=True - label=Beta Lactamase - SerialCloner_Color="&h8FBC8F" - SerialCloner_Show="True" - SerialCloner_Protect="False" - SerialCloner_Arrow="True" - note="Copied from " - label=beta Lactamase - translation="MSTFKVL \ 
     promoter        complement(4398..4426)
                     /label=Copied from _Amp prom
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Amp prom - SerialCloner_Color="&h4682B4" - SerialCloner_Show="True" - SerialCloner_Protect="False" - SerialCloner_Arrow="True" - note="Copied from " - complement(6385..6413) \ 
     Promoter        complement(4398..4426)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    987..1011
                     /label=Psup44 3 annealling
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4967..5770
                     /label=Ura
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    6031..6337
                     /label=F1_Origin
                     /SerialCloner_Color=&hFFCC66
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     624..640
                     /label=Seq for oED14
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     1003..1032
                     /label=oSP1
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        1..1002
                     /label=Psup44
                     /SerialCloner_Color=&h80FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1606..1623
                     /label=Seq rev oED15
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    1033..1059
                     /label=Ste7 DS1
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1309..2010
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    complement(1173..1182)
                     /label=Swi6NLSSP9PmutA  [*]
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ELGSPLKKLKIAPDTX" \ 
     misc_feature    complement(1269..1278)
                     /label=Swi6NLSSP9PmutA  [*]
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ELGSPLKKLKIAPDTX" \ 
     misc_feature    complement(1149..1169)
                     /label=Swi6NLSSP9PmutA  [*] [*] [3'] [3']
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ELGSPLKKLKIAPDTX" \ 
     misc_feature    complement(1245..1265)
                     /label=Swi6NLSSP9PmutA  [*] [*] [3'] [3']
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ELGSPLKKLKIAPDTX" \ 
     misc_feature    complement(1137..1146)
                     /label=Swi6NLSSP9PmutA  [*] [3']
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ELGSPLKKLKIAPDTX" \ 
     misc_feature    complement(1233..1242)
                     /label=Swi6NLSSP9PmutA  [*] [3']
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ELGSPLKKLKIAPDTX" \ 
     misc_feature    1144..1189
                     /label=Swi6NLSSP9PmutE
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ELGSPLKKLKIAPDTX" \ 
     misc_feature    1240..1285
                     /label=Swi6NLSSP9PmutE
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ELGSPLKKLKIAPDTX" \ 
                     1209..1306
                     /label=insert from oED202(NLSSP9PMutEFor2).xdna
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from oED202(NLSSP9PMutEFor2).xdna [98  nt] : (EcoRI[5] / NheI[107]) \ 
     misc_feature    complement(1233..1278)
                     /label=Swi6NLSSP9(S->E)
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 tCGTTGCCAC GATTGGAATA CCCTCAAAAT TCCCACTTTT ACTATGCTTC AATTGACCCT 
       61 TCGGTATCTC AGCATCTAGG TAGCTTTCAT TGAGATTCAT AGTTCCGATG TACTGATTCT 
      121 ACGTTTGTGT ACTTTGATAG ACTTTTTTAT TCAATTCGTA TTCACTACTA TCTTTGTCTC 
      181 GTACCGCGGT TTTTATCCTT TATTTCAGCC CTACGAGATA GAACGATGGA GCCTAGTAAT 
      241 ACCATTTTAT GTAAGCAAAA TAAGAAGAAA GACTAAAAGA ATTAGGATAT GTTGTTAATC 
      301 AGGGATAAAG GTACGCATAC TAGCCTTACT ACGTAAATAT AAGGTTACGG TTTACGATCA 
      361 CTATTGCAAG ATACTATTGT TTAGCATTGG CAAAGACTTG ACGGGAAATC TTTGATTGGG 
      421 CCCAACCATA TTCATTAAAG ATCCGGGATG TGAGCTTGAA CTGCTTTTCG ACGCGAAGAA 
      481 GGTTTACACG TATTTTAGTA ATTGGCTTAT TCACTAAGGA TTCTTAAGGT TTTCTTAATA 
      541 GTTTTCTACG TCGGCATGCG ATTGTTTGGT TTAGAAGACT GCTTTCTAAA TATGGTTGGG 
      601 TGTATTTAAG CTAGACCCAT ACACCCGCTC TATGGGATTA TTTACTTGTT TGAATTTTAA 
      661 GATTTGTGAT AATGGAACTG GACGCAAACA TTTGATGGAA AACGCATGTC ATCATTAACG 
      721 AGGTAACGTA GGTATCTGTC CTGCCTTAGT ATTGCACGCA GCTTCCCAGG ACGCCTAGCT 
      781 ATTTTTTCAT CTATTCCCCT CTGTAGTAAC GTAAGAGTTT TCAAGTTTTT AATTCAGACT 
      841 TTCTCTTCCT TTGTTTCCAA TTTCCTTCCT TACTGCTTGA TACCTTTTCA ATCCCAAAGA 
      901 AACCGTGTTC TTTATATATT GTCGATTGAA AGTTACCTAC ATCAACTTTC CGTGTTCCAT 
      961 TCCGACTATA ACAAACAACC AATAAGCTCA ACTAATTAAG TAAagctTAT GTTTCAACGA 
     1021 AAGACTTTAC AGagaaggaa cttgaaaggg ctcaatctta acctgcaccc agatgtgggc 
     1081 aataatggcc aattgcagga aaagacaTct agaCAACAAA TGGGTCGTGG TTCGGAATTT 
     1141 GAAGAACTAG GTGAGCCGCT AAAAAAACTA AAAATAGAAC CAGATACGGC TTCTGGTTTG 
     1201 GTTgaattcC AACAAATGGG TCGTGGTTCG GAATTTGAAG AACTAGGTGA GCCGCTAAAA 
     1261 AAACTAAAAA TAGAACCAGA TACGGCTTCT GGTTTGGTTa gatctgctag CAAGGGCGAG 
     1321 GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC 
     1381 GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC 
     1441 CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG 
     1501 TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC 
     1561 TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC 
     1621 GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG 
     1681 GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG 
     1741 GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC 
     1801 AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC 
     1861 AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC 
     1921 ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC 
     1981 TCCACCGGCG GCATGGACGA GCTGTACAAG GGATCTTAAC tcgagtcatg taattagtta 
     2041 tgtcacgctt acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta 
     2101 gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt 
     2161 tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta 
     2221 tactgaaaac cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcggccggt 
     2281 acccagcttt tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata 
     2341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatag gagccggaag 
     2401 cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg 
     2461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     2521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     2581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     2641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     2701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcg gcccccctga 
     2761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     2821 ataccaggcg ttcccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     2881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 
     2941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     3001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     3061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     3121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
     3181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     3241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     3301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     3361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     3421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     3481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     3541 atttcgttca tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg 
     3601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     3661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     3721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     3781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     3841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     3901 gttgtgaaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     3961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     4021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     4081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     4141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     4201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     4261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     4321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     4381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     4441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 
     4501 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 
     4561 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
     4621 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
     4681 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 
     4741 cgcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc ggtttctttg 
     4801 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact 
     4861 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt 
     4921 aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac 
     4981 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat 
     5041 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact 
     5101 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt 
     5161 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa 
     5221 ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta 
     5281 ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt 
     5341 gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag 
     5401 aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac 
     5461 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca 
     5521 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg 
     5581 tttagatgac aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc 
     5641 tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa 
     5701 ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca 
     5761 gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc 
     5821 ttcaatttaa ttatatcagt tattaccctg cggtgtgaaa taccgcacag atgcgtaagg 
     5881 agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt 
     5941 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 
     6001 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 
     6061 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 
     6121 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 
     6181 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 
     6241 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 
     6301 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc 
     6361 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
     6421 tacgccagct ggcgaagggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
     6481 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg 
     6541 gcgaattgga gc
//