LOCUS       pSP303_pRS306_Far1DS_2xNLS-SP9_Cherry.xdna    6558 bp      DNA     circular   23.07.15
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pSP 301 [5437  nt] : (XbaI[3660] / SacI[2063])
             to pSP288_pRS305_Far1DS_2xNLS-SP9_Venus.xdna [1113  nt] : (
            SacI[3124] / XbaI[4241])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    4489..2066
                     /label=insert from pED40(DS1p33- NLSSP8).xdna [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED40(DS1p33- NLSSP8).xdna [6452  nt] : (BglII[3023] / EcoRI[3014]) \ 
     misc_feature    complement(267..281)
                     /label=pED1-6 Ann.Seq [3']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
     misc_feature    334..345
                     /label=pED1-6 Ann.Seq [5']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
     misc_feature    355..358
                     /label=pED1-6 Ann.Seq [3']
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ASGLVX" \ 
                     362..1368
                     /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356  nt] : (BglII[3203] / XbaI[3104]) \ 
     misc_feature    4489..256
                     /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [3'] [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356  nt] : (BglII[3203] / XbaI[3104]) \ 
     misc_feature    3053..3077
                     /label=Psup44 3 annealling
                     /SerialCloner_Color=&h6666FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             481..1284
                     /label=Ura
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1545..1851
                     /label=F1_Origin
                     /SerialCloner_Color=&hFFCC66
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     2690..2706
                     /label=Seq for oED14
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        2067..3068
                     /label=Psup44
                     /SerialCloner_Color=&h80FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3678..3695
                     /label=Seq rev oED15
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3381..4082
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3216..3261
                     /label=Swi6NLSSP8
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3312..3357
                     /label=Swi6NLSSP8
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3312..3357
                     /label=Swi6NLSSP9
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     2012..2029
                     /label=M13-fwd
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     2036..2055
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      1423..1863
                     /label=F1 ori
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(1413..1868)
                     /label=M13 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(1873..1941)
                     /label=LacZ alpha
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
                     2068..3180
                     /label=insert from pSP288_pRS305_Far1DS_2xNLS-SP9_Venus.xdna
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from pSP288_pRS305_Far1DS_2xNLS-SP9_Venus.xdna [1113  nt] : (SacI[3124] / XbaI[4241]) \ 
     misc_feature    2069..3068
                     /label=pRPS2 promoter
                     /SerialCloner_Color=&h004080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3186..3371
                     /label=2xNLS SP9
                     /SerialCloner_Color=&h004080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3216..3261
                     /label=Swi6NLSSP9
                     /SerialCloner_Color=&h8000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(4366..4385)
                     /label=T3
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4403..4423)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4791..5419)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(4429..4451)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5571..6230)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5571..6230)
                     /label=beta Lactamase
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=dnas_title="Beta Lactamase" - codon_start=True - label=Beta Lactamase - SerialCloner_Color="&h8FBC8F" - SerialCloner_Show="True" - SerialCloner_Protect="False" - SerialCloner_Arrow="True" - note="Copied from " - label=beta Lactamase - translation="MSTFKVL \ 
     promoter        complement(6470..6498)
                     /label=Copied from _Amp prom
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Amp prom - SerialCloner_Color="&h4682B4" - SerialCloner_Show="True" - SerialCloner_Protect="False" - SerialCloner_Arrow="True" - note="Copied from " - complement(6385..6413) \ 
     Promoter        complement(6470..6498)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3075..3179
                     /label=Far1 Dock
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 
       61 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 
      121 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 
      181 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 
      241 ctgagagtgc accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga 
      301 tttcggtttc tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg 
      361 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat 
      421 tgcccagtat tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc 
      481 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag 
      541 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc 
      601 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca 
      661 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta 
      721 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca 
      781 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat 
      841 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta 
      901 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct 
      961 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc 
     1021 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg 
     1081 acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg 
     1141 gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag 
     1201 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg 
     1261 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac 
     1321 tcacaaatta gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc 
     1381 acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa 
     1441 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 
     1501 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 
     1561 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 
     1621 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 
     1681 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 
     1741 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 
     1801 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 
     1861 gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 
     1921 ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg 
     1981 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata 
     2041 cgactcacta tagggcgaat tggagctCGT TGCCACGATT GGAATACCCT CAAAATTCCC 
     2101 ACTTTTACTA TGCTTCAATT GACCCTTCGG TATCTCAGCA TCTAGGTAGC TTTCATTGAG 
     2161 ATTCATAGTT CCGATGTACT GATTCTACGT TTGTGTACTT TGATAGACTT TTTTATTCAA 
     2221 TTCGTATTCA CTACTATCTT TGTCTCGTAC CGCGGTTTTT ATCCTTTATT TCAGCCCTAC 
     2281 GAGATAGAAC GATGGAGCCT AGTAATACCA TTTTATGTAA GCAAAATAAG AAGAAAGACT 
     2341 AAAAGAATTA GGATATGTTG TTAATCAGGG ATAAAGGTAC GCATACTAGC CTTACTACGT 
     2401 AAATATAAGG TTACGGTTTA CGATCACTAT TGCAAGATAC TATTGTTTAG CATTGGCAAA 
     2461 GACTTGACGG GAAATCTTTG ATTGGGCCCA ACCATATTCA TTAAAGATCC GGGATGTGAG 
     2521 CTTGAACTGC TTTTCGACGC GAAGAAGGTT TACACGTATT TTAGTAATTG GCTTATTCAC 
     2581 TAAGGATTCT TAAGGTTTTC TTAATAGTTT TCTACGTCGG CATGCGATTG TTTGGTTTAG 
     2641 AAGACTGCTT TCTAAATATG GTTGGGTGTA TTTAAGCTAG ACCCATACAC CCGCTCTATG 
     2701 GGATTATTTA CTTGTTTGAA TTTTAAGATT TGTGATAATG GAACTGGACG CAAACATTTG 
     2761 ATGGAAAACG CATGTCATCA TTAACGAGGT AACGTAGGTA TCTGTCCTGC CTTAGTATTG 
     2821 CACGCAGCTT CCCAGGACGC CTAGCTATTT TTTCATCTAT TCCCCTCTGT AGTAACGTAA 
     2881 GAGTTTTCAA GTTTTTAATT CAGACTTTCT CTTCCTTTGT TTCCAATTTC CTTCCTTACT 
     2941 GCTTGATACC TTTTCAATCC CAAAGAAACC GTGTTCTTTA TATATTGTCG ATTGAAAGTT 
     3001 ACCTACATCA ACTTTCCGTG TTCCATTCCG ACTATAACAA ACAACCAATA AGCTCAACTA 
     3061 ATTAAGTaaa gcttATGTTT CAACGAAAGA CTTTACAGAA AAGAGGCAAT ATTCCTAAAC 
     3121 CACTGAATCT TTCCCTGCAC CCAGATGTGG GCAATAATGG CCAATTGCAG GAAAAGACAt 
     3181 ctagaCAACA AATGGGTCGT GGTTCGGAAT TTGAAGAACT AGGTTCCCCG CTAAAAAAAC 
     3241 TAAAAATATC ACCAGATACG GCTTCTGGTT TGGTTgaatt cCAACAAATG GGTCGTGGTT 
     3301 CGGAATTTGA AGAACTAGGT TCCCCGCTAA AAAAACTAAA AATATCACCA GATACGGCTT 
     3361 CTGGTTTGGT TagatcTGCT AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT 
     3421 TCATGCGCTT CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG 
     3481 GCGAGGGCGA GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG 
     3541 GTGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG 
     3601 CCTACGTGAA GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT 
     3661 TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT 
     3721 CCTCCCTGCA GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT 
     3781 CCGACGGCCC CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT 
     3841 ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG 
     3901 GCCACTACGA CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG 
     3961 GCGCCTACAA CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG 
     4021 TGGAACAGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA 
     4081 AGGGATCTTA ACtcgagtca tgtaattagt tatgtcacgc ttacattcac gccctccccc 
     4141 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta 
     4201 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt 
     4261 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt 
     4321 gggacgctcg aaggctttaa tttgcggccg gtacccagct tttgttccct ttagtgaggg 
     4381 ttaattccga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     4441 ctcacaattc cacacaacat aggagccgga agcataaagt gtaaagcctg gggtgcctaa 
     4501 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     4561 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     4621 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     4681 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     4741 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     4801 ctggcgtttt tccataggct cggcccccct gacgagcatc acaaaaatcg acgctcaagt 
     4861 cagaggtggc gaaacccgac aggactataa agataccagg cgttcccccc tggaagctcc 
     4921 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     4981 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 
     5041 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     5101 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     5161 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     5221 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     5281 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     5341 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     5401 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     5461 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     5521 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     5581 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactg 
     5641 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     5701 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     5761 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     5821 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     5881 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     5941 caacgatcaa ggcgagttac atgatccccc atgttgtgaa aaaaagcggt tagctccttc 
     6001 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     6061 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     6121 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     6181 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     6241 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     6301 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     6361 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     6421 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     6481 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     6541 ccccgaaaag tgccacct
//