LOCUS pSP323_pRS306_Mkk2_1-100_2xNLS_Cherry.xdna 6753 bp DNA circular 30.06.15 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=Degen. DNA COMMENT SerialCloner_Comments=Ligation of : Ligation9_pRS306_Mkk2_1-150_NLS_Cherry.xdna [6 443 nt] : (XbaI[3525] / HindIII[3069]) to PCR(Mkk2_1kbUD.xd na) [302 nt] : (HindIII[7] / XbaI[313]) COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers misc_feature 4684..2066 /label=insert from pED40(DS1p33- NLSSP8).xdna [*] [3'] /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=False /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from pED40(DS1p33- NLSSP8).xdna [6452 nt] : (BglII[3023] / EcoRI[3014]) \ misc_feature 362..1368 /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [5'] /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=False /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356 nt] : (BglII[3203] / XbaI[3104]) \ misc_feature 4684..256 /label=insert from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [*] [3'] [*] [3'] /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=False /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from pED30(Ste7DS1(1-33),Swi6NLS(SP)).xdna [6356 nt] : (BglII[3203] / XbaI[3104]) \ misc_feature 3053..3077 /label=Psup44 3 annealling /SerialCloner_Color=&h6666FF /SerialCloner_Show=False /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 481..1284 /label=Ura /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1545..1851 /label=F1_Origin /SerialCloner_Color=&hFFCC66 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 2690..2706 /label=Seq for oED14 /SerialCloner_Color=&h00FFFF /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True promoter 2067..3068 /label=pRPS2 /SerialCloner_Color=&h80FF00 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3873..3890 /label=Seq rev oED15 /SerialCloner_Color=&h00FFFF /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3576..4277 /label=mCherry /SerialCloner_Color=&hFF0000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3507..3552 /label=Swi6NLSSP8 /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3381..3566 /label=2xNLS SP9 /SerialCloner_Color=&h004080 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True misc_feature 3507..3552 /label=Swi6NLSSP9 /SerialCloner_Color=&h8000FF /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True primer_bind complement(4561..4580) /label=T3 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 2012..2029 /label=M13-fwd /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(4598..4618) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 2036..2055 /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(4986..5614) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin 1423..1863 /label=F1 ori /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(1413..1868) /label=M13 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(1873..1941) /label=LacZ alpha /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(4624..4646) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(5766..6425) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(5766..6425) /label=beta Lactamase /SerialCloner_Color=&h84A4C0 /SerialCloner_Show=False /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=dnas_title="Beta Lactamase" - codon_start=True - label=Beta Lactamase - SerialCloner_Color="&h8FBC8F" - SerialCloner_Show="True" - SerialCloner_Protect="False" - SerialCloner_Arrow="True" - note="Copied from " - label=beta Lactamase - translation="MSTFKVL \ promoter complement(6665..6693) /label=Copied from _Amp prom /SerialCloner_Color=&hF08080 /SerialCloner_Show=False /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=label=Amp prom - SerialCloner_Color="&h4682B4" - SerialCloner_Show="True" - SerialCloner_Protect="False" - SerialCloner_Arrow="True" - note="Copied from " - complement(6385..6413) \ Promoter complement(6665..6693) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=False /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3074..3375 /label=Mkk2 DS 1-100 /SerialCloner_Color=&h804000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="Fragment extracted from PCR(Mkk2_1kbUD.xdna) [302 nt] : (HindIII[7] / XbaI[313]) \ misc_feature 3075..3173 /label=DS pSP306 /SerialCloner_Color=&hFF0000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation"translation="MASMFRPPESNRSHQKTPKLTLPVNLVQNAKST" \ misc_feature 3132..3140 /label=LTL docking /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation"translation="LTL" \ ORIGIN 1 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 61 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 121 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 181 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 241 ctgagagtgc accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga 301 tttcggtttc tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg 361 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat 421 tgcccagtat tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc 481 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag 541 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc 601 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca 661 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta 721 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca 781 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat 841 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta 901 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct 961 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc 1021 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg 1081 acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg 1141 gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag 1201 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg 1261 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac 1321 tcacaaatta gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc 1381 acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa 1441 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 1501 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 1561 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 1621 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 1681 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 1741 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 1801 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 1861 gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 1921 ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc gattaagttg 1981 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata 2041 cgactcacta tagggcgaat tggagctCGT TGCCACGATT GGAATACCCT CAAAATTCCC 2101 ACTTTTACTA TGCTTCAATT GACCCTTCGG TATCTCAGCA TCTAGGTAGC TTTCATTGAG 2161 ATTCATAGTT CCGATGTACT GATTCTACGT TTGTGTACTT TGATAGACTT TTTTATTCAA 2221 TTCGTATTCA CTACTATCTT TGTCTCGTAC CGCGGTTTTT ATCCTTTATT TCAGCCCTAC 2281 GAGATAGAAC GATGGAGCCT AGTAATACCA TTTTATGTAA GCAAAATAAG AAGAAAGACT 2341 AAAAGAATTA GGATATGTTG TTAATCAGGG ATAAAGGTAC GCATACTAGC CTTACTACGT 2401 AAATATAAGG TTACGGTTTA CGATCACTAT TGCAAGATAC TATTGTTTAG CATTGGCAAA 2461 GACTTGACGG GAAATCTTTG ATTGGGCCCA ACCATATTCA TTAAAGATCC GGGATGTGAG 2521 CTTGAACTGC TTTTCGACGC GAAGAAGGTT TACACGTATT TTAGTAATTG GCTTATTCAC 2581 TAAGGATTCT TAAGGTTTTC TTAATAGTTT TCTACGTCGG CATGCGATTG TTTGGTTTAG 2641 AAGACTGCTT TCTAAATATG GTTGGGTGTA TTTAAGCTAG ACCCATACAC CCGCTCTATG 2701 GGATTATTTA CTTGTTTGAA TTTTAAGATT TGTGATAATG GAACTGGACG CAAACATTTG 2761 ATGGAAAACG CATGTCATCA TTAACGAGGT AACGTAGGTA TCTGTCCTGC CTTAGTATTG 2821 CACGCAGCTT CCCAGGACGC CTAGCTATTT TTTCATCTAT TCCCCTCTGT AGTAACGTAA 2881 GAGTTTTCAA GTTTTTAATT CAGACTTTCT CTTCCTTTGT TTCCAATTTC CTTCCTTACT 2941 GCTTGATACC TTTTCAATCC CAAAGAAACC GTGTTCTTTA TATATTGTCG ATTGAAAGTT 3001 ACCTACATCA ACTTTCCGTG TTCCATTCCG ACTATAACAA ACAACCAATA AGCTCAACTA 3061 ATTAAGTAAa gctTATGGCT TCAATGTTCA GACCACCAGA ATCCAATAGG AGTCACCAAA 3121 AGACTCCAAA ATTAACGCTT CCAGTAAATT TAGTTCAAAA TGCGAAATCC ACTAATGATG 3181 GGCAACATCT CAACCGGTCA CCGTACTCGT CAGTGAACGA AAGCCCATAC TCCAACAATA 3241 GCACTTCAGC TACTTCCACT ACGTCATCCA TGGCTTCAAA TTCCACTTTG TTGTACAATA 3301 GATCATCTAC TACAACTATT AAAAATAGAC CGGTACCACC TCCATTACCT CCCCTAGTAC 3361 TAACGCAAAA AAAATctaga CAACAAATGG GTCGTGGTTC GGAATTTGAA GAACTAGGTT 3421 CCCCGCTAAA AAAACTAAAA ATATCACCAG ATACGGCTTC TGGTTTGGTT gaattcCAAC 3481 AAATGGGTCG TGGTTCGGAA TTTGAAGAAC TAGGTTCCCC GCTAAAAAAA CTAAAAATAT 3541 CACCAGATAC GGCTTCTGGT TTGGTTagat cTGCTAGCAA GGGCGAGGAG GATAACATGG 3601 CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG AACGGCCACG 3661 AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC 3721 TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCTCAGTTCA 3781 TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC TTGAAGCTGT 3841 CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCGTGGTGA 3901 CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG AAGCTGCGCG 3961 GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT 4021 CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG CAGAGGCTGA 4081 AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG GCCAAGAAGC 4141 CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC TCCCACAACG 4201 AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA 4261 TGGACGAGCT GTACAAGGGA TCTTAACtcg agtcatgtaa ttagttatgt cacgcttaca 4321 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt 4381 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca 4441 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt 4501 gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggccggtacc cagcttttgt 4561 tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg 4621 tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa 4681 gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct 4741 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 4801 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 4861 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 4921 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 4981 aaaaaggccg cgttgctggc gtttttccat aggctcggcc cccctgacga gcatcacaaa 5041 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttc 5101 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5161 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 5221 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5281 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5341 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5401 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5461 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 5521 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 5581 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 5641 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 5701 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 5761 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 5821 atagttgcct gactgcccgt cgtgtagata actacgatac gggagggctt accatctggc 5881 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 5941 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6001 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6061 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6121 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgaaaaaaa 6181 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6241 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6301 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6361 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 6421 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 6481 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 6541 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 6601 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 6661 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 6721 gttccgcgca catttccccg aaaagtgcca cct //