LOCUS       pcDNA3.1(+)\phiC        6698 bp    DNA     circular SYN  9-JUL-2009
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|516716260|
COMMENT     VNTDBDATE|523377867|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA3.1(+) phiC31|
COMMENT     VNTAUTHORNAME|Christian Mosimann|
FEATURES             Location/Qualifiers
     source          1..6698
                     /invitrogen="124"
                     /vntifkey="98"
     CDS             complement(5702..6562)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_site      2298..2522
                     /invitrogen="1870000"
                     /vntifkey="26"
                     /label=BGH\pA
     primer          complement(2292..2309)
                     /invitrogen="1910000"
                     /vntifkey="27"
                     /label=BGH\reverse\primer
     promoter        complement(6563..6661)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     primer          769..789
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     promoter        232..819
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     rep_origin      2568..2996
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     misc_feature    895..2280
                     /invitrogen="1630000"
                     /vntifkey="21"
                     /label=MCS
     CDS             3406..4200
                     /invitrogen="1260000"
                     /vntifkey="4"
                     /label=Neo(R)
     rep_origin      complement(4887..5557)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     promoter        3001..3370
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     polyA_signal    4374..4504
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     primer          863..882
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     promoter        863..879
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     CDS             1072..2139
                     /vntifkey="4"
                     /label=phiC31\ORF
BASE COUNT     1541 a      1795 c      1802 g      1560 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt agcttgcgca ctcgaccact ccccttaccg gacccttcga gttcgagccc 
      961 gacgtaatcc ggtggtggtg gcgtgagatc aagacgcaca aacaccttcc cttcaagccg 
     1021 ggcagtcaag ccgccattca cccgggcagc atcacggggc tttgtaagcg catggacgct 
     1081 gacgccgtgc cgacccgggg cgagacgatt gggaagaaga ccgcttcaag cgcctgggac 
     1141 ccggcaaccg ttatgcgaat ccttcgggac ccgcgtattg cgggcttcgc cgctgaggtg 
     1201 atctacaaga agaagccgga cggcacgccg accacgaaga ttgagggtta ccgcattcag 
     1261 cgcgacccga tcacgctccg gccggtcgag cttgattgcg gaccgatcat cgagcccgct 
     1321 gagtggtatg agcttcaggc gtggttggac ggcagggggc gcggcaaggg gctttcccgg 
     1381 gggcaagcca ttctgtccgc catggacaag ctgtactgcg agtgtggcgc cgtcatgact 
     1441 tcgaagcgcg gggaagaatc gatcaaggac tcttaccgct gccgtcgccg gaaggtggtc 
     1501 gacccgtccg cacctgggca gcacgaaggc acgtgcaacg tcagcatggc ggcactcgac 
     1561 aagttcgttg cggaacgcat cttcaacaag atcaggcacg ccgaaggcga cgaagagacg 
     1621 ttggcgcttc tgtgggaagc cgcccgacgc ttcggcaagc tcactgaggc gcctgagaag 
     1681 agcggcgaac gggcgaacct tgttgcggag cgcgccgacg ccctgaacgc ccttgaagag 
     1741 ctgtacgaag accgcgcggc aggcgcgtac gacggacccg ttggcaggaa gcacttccgg 
     1801 aagcaacagg cagcgctgac gctccggcag caaggggcgg aagagcggct tgccgaactt 
     1861 gaagccgccg aagccccgaa gcttcccctt gaccaatggt tccccgaaga cgccgacgct 
     1921 gacccgaccg gccctaagtc gtggtggggg cgcgcgtcag tagacgacaa gcgcgtgttc 
     1981 gtcgggctct tcgtagacaa gatcgttgtc acgaagtcga ctacgggcag ggggcaggga 
     2041 acgcccatcg agaagcgcgc ttcgatcacg tgggcgaagc cgccgaccga cgacgacgaa 
     2101 gacgacgccc aggacggcac ggaagacgta gcggcgtagc gagacacccg gatcgcgcca 
     2161 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ccggatccgg atccactagt ccagtgtggt 
     2221 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac 
     2281 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 
     2341 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 
     2401 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 
     2461 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 
     2521 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 
     2581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     2641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     2701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     2761 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     2821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     2881 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
     2941 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 
     3001 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 
     3061 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
     3121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
     3181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     3241 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 
     3301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 
     3361 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 
     3421 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 
     3481 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 
     3541 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 
     3601 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 
     3661 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 
     3721 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 
     3781 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 
     3841 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 
     3901 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 
     3961 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 
     4021 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 
     4081 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 
     4141 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 
     4201 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 
     4261 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 
     4321 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 
     4381 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     4441 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     4501 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 
     4561 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     4621 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     4681 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     4741 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     4801 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     4861 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     4921 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     4981 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     5041 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     5101 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     5161 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     5221 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     5281 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     5341 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 
     5401 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     5461 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     5521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     5581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     5641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
     5701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
     5761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
     5821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
     5881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
     5941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
     6001 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
     6061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
     6121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
     6181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
     6241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
     6301 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
     6361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
     6421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
     6481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
     6541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
     6601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
     6661 ttccgcgcac atttccccga aaagtgccac ctgacgtc 
//
