LOCUS       pCM254_pENTR5'-attP      2936 bp    DNA     circular SYN 29-MAY-2013
DEFINITION  pENTR5 TOPO Sequence sequence
ACCESSION   pCM254 pENTR5'-attP
DBSOURCE    GenBank
SOURCE      Danio rerio (unknown)
  ORGANISM  Danio rerio
            Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Cyprinidae; Danio
COMMENT     See Addgene Vectorpedia pENTR5 TOPO Sequence sequence at www.addgene.org
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Addgene Vectorpedia pENTR5 TOPO Sequence
                     sequence"
                     /mol_type="other DNA"
                     /note="Oriinal location: 1..2680"
                     /label=Source_1
     terminator      complement(268..295)
                     /note="Oriinal location: complement(268..295)"
                     /label=rrnB_T2_terminator
     terminator      complement(427..470)
                     /note="Oriinal location: complement(427..470)"
                     /label=rrnB_T1_terminator
     misc_feature    522..544
                     /note="Oriinal location: 522..544"
                     /label=M13_pUC_fwd_primer
     misc_feature    537..553
                     /note="Oriinal location: 537..553"
                     /label=M13_forward20_primer
     misc_feature    complement(592..675)
                     /note="Oriinal location: complement(592..675)"
                     /label=attL4
     misc_signal     726..946
                     /note="Oriinal location: 3090..3310"
                     /note="attP integration site"
                     /label=attP
     misc_feature    963
                     /note="Insertion site between two As."
                     /label="TOPO Insert"
     misc_feature    complement(1001..1084)
                     /note="Oriinal location: complement(745..828)"
                     /label=attR1
     promoter        complement(1178..1196)
                     /note="Oriinal location: complement(922..940)"
                     /label=T7_promoter
     misc_feature    complement(1197..1215)
                     /note="Oriinal location: complement(941..959)"
                     /label=M13_reverse_primer
     CDS             1330..2139
                     /gene="KanR2"
                     /note="Oriinal location: 1074..1883"
                     /note="Orf frame 3"
     gene            1330..2139
                     /gene="KanR2"
                     /note="Oriinal location: 1074..1883"
     rep_origin      2270..2889
                     /note="Oriinal location: 2014..2633"
                     /label=pBR322_origin
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCGA GTTAACGCTA CCATGGAGCT CCAAATAATG
      601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
      661 ATAATGCCAA CTTTGTATAG AAAAGTTGGC TCCGAATTCG CCCTTCACCT CTCGAGGTAC
      721 CTGCAGTACT GACGGACACA CCGAAGCCCC GGCGGCAACC CTCAGCGGAT GCCCCGGGGC
      781 TTCACGTTTT CCCAGGTCAG AAGCGGTTTT CGGGAGTAGT GCCCCAACTG GGGTAACCTT
      841 TGAGTTCTCT CAGTTGGGGG CGTAGGGTCG CCGACATGAC ACAAGGGGTT GTGACCGGGG
      901 TGGACACGTA CGCGGGTGCT TACGACCGTC AGTCGCGCGA GCGCGATCTA GAGTCGTCGA
      961 CAAGGGCGAA TTCGACCCAA GTTTGTACAA AAAAGTTGAA CGAGAAACGT AAAATGATAT
     1021 AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC
     1081 ACAACATATG CAGTCACTAT GAACCAACTA CTTAGATGGT ATTAGTGACC TGTAGAATTA
     1141 ATTCGAGCTC TAGAGCTGCA GGGCGGCCGC GATATCCCCT ATAGTGAGTC GTATTACATG
     1201 GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA
     1261 CAAGATAAAA ATATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA
     1321 AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG
     1381 GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA
     1441 ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT
     1501 AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG
     1561 CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT
     1621 GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT
     1681 ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT
     1741 CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT
     1801 TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG
     1861 AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC
     1921 TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA
     1981 GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT
     2041 TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT
     2101 AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG
     2161 TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC
     2221 CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA
     2281 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG
     2341 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT
     2401 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC
     2461 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG
     2521 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG
     2581 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA
     2641 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC
     2701 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG
     2761 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC
     2821 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC
     2881 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT
//
