LOCUS       pCM268\pDest/att        5085 bp    DNA     circular SYN 24-MAR-2009
DEFINITION  Contig[0033], 7418 bases, 6140 checksum.
ACCESSION   -
KEYWORDS    -.
SOURCE      -.
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|513627026|
COMMENT     VNTDBDATE|513627026|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCM268 pDest/attB|
COMMENT     VNTAUTHORNAME|Christian Mosimann|
FEATURES             Location/Qualifiers
     gene            667..746
                     /vntifkey="60"
                     /note="white"
     gene            4100..4957
                     /vntifkey="60"
                     /note="Amp"
     misc_signal     2588..2872
                     /vntifkey="87"
                     /label=attB
                     /note="attB"
     frag            3..459
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:frag  1 to 457 of f1 + orientation cass."
     frag            complement(3..459)
                     /vntifkey="21"
                     /label=Misc_Feature_2
                     /note="Unknown feature type:frag  6 to 462 of pUAS-lacZattB copy"
     source          complement(<3..>459)
                     /organism="synthetic construct"
                     /db_xref="taxon:32630 [Split]"
                     /vntifkey="98"
                     /note="mol_type=other DNA"
     misc_feature    complement(<3..>459)
                     /vntifkey="21"
                     /label=Misc_Feature_3
                     /note="phagemid pBluescriptII SK(-) [Split]"
     misc_recomb     2416..2540
                     /ApEinfo_fwdcolor=#5370ff
                     /ApEinfo_revcolor=#5370ff
                     /vntifkey="86"
                     /label=attR3
     misc_recomb     837..961
                     /ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_revcolor=#5d40ff
                     /vntifkey="86"
                     /label=attR4
     misc_feature    2520..2534
                     /ApEinfo_fwdcolor=#4259cc
                     /ApEinfo_revcolor=#4259cc
                     /vntifkey="21"
                     /label=att3_shared
     misc_feature    837..857
                     /ApEinfo_fwdcolor=#4a33cc
                     /ApEinfo_revcolor=#4a33cc
                     /vntifkey="21"
                     /label=att4_shared
     CDS             complement(1648..2307)
                     /ApEinfo_fwdcolor=#ffbf00
                     /ApEinfo_revcolor=#ffbf00
                     /vntifkey="4"
                     /label=cmR
     misc_feature    complement(1001..1306)
                     /ApEinfo_fwdcolor=#ffbf3f
                     /ApEinfo_revcolor=#ffbf3f
                     /vntifkey="21"
                     /label=ccdB
     primer_bind     801..822
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13R
     primer_bind     complement(2549..2564)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13F
BASE COUNT     1315 a      1238 c      1231 g      1301 t 
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg 
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa 
      721 catttttttt ttttactgca ctggatctag cggatccggg aattgggaat tcgttaacag 
      781 atctgcggcc gcggctcgag caggaaacag ctatgaccat gattacgcca agctatcaac 
      841 tttgtataga aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta 
      901 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg 
      961 gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca 
     1021 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga 
     1081 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga 
     1141 tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt gcactggcca 
     1201 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca 
     1261 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg 
     1321 ttctcgtcgg caaaggagcc gttcatttca ataaaccggg cgacctcagc catcccttcc 
     1381 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg 
     1441 cttaccgaac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt 
     1501 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata 
     1561 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct 
     1621 ggcttttagt aagccggatc ctctagatta cgccccgccc tgccactcat cgcagtactg 
     1681 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg 
     1741 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac 
     1801 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca 
     1861 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt 
     1921 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg 
     1981 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg 
     2041 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg 
     2101 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt 
     2161 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg 
     2221 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt 
     2281 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg 
     2341 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggg 
     2401 tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg 
     2461 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc 
     2521 aactttatta tacatagttg ataattcact ggccgtcgtt ttacggtacc tctagagatc 
     2581 cactagtgtc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc 
     2641 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt 
     2701 cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga 
     2761 aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg 
     2821 atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acactagttc 
     2881 tagccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat 
     2941 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 
     3001 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 
     3061 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 
     3121 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 
     3181 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
     3241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     3301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     3361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     3421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     3481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     3541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     3601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     3661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     3721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 
     3781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     3841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     3901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     3961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     4021 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     4081 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     4141 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     4201 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     4261 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     4321 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     4381 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 
     4441 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     4501 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     4561 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     4621 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     4681 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 
     4741 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     4801 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     4861 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     4921 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     4981 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     5041 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac 
//
