LOCUS       1._Corynebacter_       10448 bp    DNA     circular     09-APR-2013
DEFINITION  .
ACCESSION   urn.local...1365455697109.7223
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1468..1487
                     /note="Geneious type: motif"
                     /label=H1.3F
     misc_feature    1564..1583
                     /note="Geneious type: motif"
                     /label=pRNA-CMV-F
     misc_feature    1570..1591
                     /note="Geneious type: motif"
                     /label=pEGFP-N-5'
     misc_feature    1615..1633
                     /note="Geneious type: motif"
                     /label=T7 promotor
     misc_feature    1810..5145
                     /label=CodiCas9
     misc_feature    complement(5172..5190)
                     /note="Geneious type: motif"
                     /label=SP6
     misc_feature    9526..10386
                     /note="Geneious type: motif"
                     /label=AMP
     misc_feature    9526..10386
                     /note="Geneious type: motif"
                     /label=ampr
     misc_feature    9962..9981
                     /note="Geneious type: motif"
                     /label=SiRNA5'
ORIGIN      
          1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
         61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
        121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
        181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
        241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
        301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
        361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
        421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
        481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
        541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
        601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
        661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
        721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
        781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
        841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
        901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
        961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
       1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
       1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
       1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
       1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
       1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
       1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
       1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
       1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
       1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
       1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
       1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
       1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
       1861 aaaaagcgca aggtcgaagc gtccatgaag taccatgtcg gaatcgatgt cggaaccttt
       1921 tctgtggggc tggctgctat tgaagtggat gacgctggaa tgcctattaa gaccctgagt
       1981 ctggtgtcac acattcatga ctcaggactg gatcctgacg agatcaagag cgctgtgacc
       2041 aggctggcaa gctccggaat cgcccggaga acaaggcgcc tgtaccgacg gaagagaagg
       2101 cgcctgcagc agctggataa gttcatccag aggcagggct ggccagtgat cgagctggaa
       2161 gattacagcg accccctgta tccttggaag gtgcgcgccg aactggccgc ttcttatatt
       2221 gctgacgaga aggaacgggg ggagaaactg agtgtggctc tgagacacat cgcaaggcat
       2281 cgcggatgga ggaaccctta cgccaaggtg tctagtctgt atctgccaga tggcccctca
       2341 gacgccttca aggctattag ggaggaaatc aaacgcgcta gcggccagcc tgtgccagag
       2401 actgcaaccg tcgggcagat ggtgaccctg tgcgaactgg gcacactgaa gctgcgagga
       2461 gagggaggag tgctgagtgc acggctgcag cagtcagatt acgcccgcga gatccaggaa
       2521 atttgtcgaa tgcaggagat cggccaggaa ctgtatcgca agatcattga cgtggtgttc
       2581 gcagccgagt ccccaaaggg ctctgcctca agccgggtgg ggaaagatcc tctgcagcca
       2641 ggaaagaaca gagcactgaa agccagcgac gcttttcagc gataccggat tgctgcactg
       2701 atcggcaatc tgagagtcag ggtggatggg gagaagagga ttctgagcgt ggaggagaag
       2761 aacctggtgt tcgaccacct ggtgaatctg actccaaaga aagagcccga atgggtgacc
       2821 atcgccgaaa ttctgggcat cgatcgcggg cagctgatcg gaacagctac tatgaccgac
       2881 gatggagagc gagcaggagc ccgaccccct acacacgata ctaacagaag tattgtgaac
       2941 agccggatcg caccactggt cgactggtgg aaaacagcta gcgcactgga gcagcacgcc
       3001 atggtgaagg cactgtccaa cgccgaagtc gacgattttg attctcccga gggagcaaaa
       3061 gtgcaggcat tctttgccga tctggacgat gacgtccacg ccaagctgga cagcctgcat
       3121 ctgcctgtgg gacgagcagc ttactccgag gacactctgg tcagactgac ccgacggatg
       3181 ctgagtgatg gggtggacct gtataccgcc cggctgcagg agttcggaat tgaacctagc
       3241 tggaccccac ccacaccaag aatcggagag cctgtcggca atccagccgt cgaccgggtg
       3301 ctgaaaacag tgagcagatg gctggaatcc gcaacaaaga cttggggcgc cccagagagg
       3361 gtcatcattg agcacgtgcg cgaaggcttc gtcactgaga aacgcgctcg agaaatggat
       3421 ggggacatga gaaggcgcgc agcccggaac gccaagctgt ttcaggagat gcaggaaaag
       3481 ctgaatgtgc agggcaaacc cagtcgagcc gatctgtgga gataccagtc agtgcagaga
       3541 cagaactgcc agtgtgccta ttgcgggtcc ccaattacct tttctaatag tgaaatggac
       3601 cacatcgtgc ccagagcagg gcagggatcc accaacacaa gggagaatct ggtcgccgtg
       3661 tgccatcgct gtaaccagtc taagggcaat acacccttcg ctatttgggc aaaaaacact
       3721 tctatcgaag gggtcagtgt gaaggaggcc gtggaacgga ccagacattg ggtcactgat
       3781 accggcatga gaagcactga cttcaagaag ttcaccaagg ctgtggtcga gcggtttcag
       3841 agagcaacaa tggatgagga aatcgacgcc agaagcatgg aatccgtcgc ctggatggct
       3901 aatgagctga ggagccgcgt ggctcagcac ttcgcatccc atggaaccac agtcagggtg
       3961 taccgaggca gcctgacagc agaggctcga cgggcatctg ggatcagtgg aaagctgaaa
       4021 ttctttgatg gcgtggggaa gtccaggctg gatagaaggc accatgctat tgacgctgca
       4081 gtgatcgcat tcacctctga ctatgtggcc gaaacactgg ctgtccgctc aaacctgaaa
       4141 cagagccagg cccaccgaca ggaggctcct cagtggagag agttcaccgg caaggatgca
       4201 gagcatcgag cagcttggag agtgtggtgc cagaagatgg aaaaactgag cgccctgctg
       4261 accgaggacc tgcgagatga ccgggtggtc gtgatgtcta acgtgcgact gcggctggga
       4321 aatggcagtg cccacaagga aaccattggc aaactgtcaa aggtgaaact gtcctctcag
       4381 ctgtcagtca gcgatatcga caaagcaagt tcagaggccc tgtggtgtgc tctgaccaga
       4441 gagcccggat tcgatcctaa ggaaggcctg cccgctaacc ctgagagaca catcagggtg
       4501 aatgggacac atgtctacgc cggggacaat attggactgt ttccagtgtc agcaggaagc
       4561 atcgcactga ggggaggata cgcagagctg ggcagctcct tccaccatgc tcgcgtgtat
       4621 aaaattactt ccggcaagaa acccgcattt gccatgctga gggtgtacac catcgatctg
       4681 ctgccttatc gcaaccagga cctgtttagc gtggaactga agccacagac aatgtccatg
       4741 aggcaggctg agaagaaact gcgcgacgct ctggcaactg ggaatgcaga atatctggga
       4801 tggctggtcg tggatgacga gctggtcgtg gatacatcta agattgccac tgaccaggtc
       4861 aaagcagtgg aggccgaact ggggactatc cgccgatggc gggtggatgg attcttttcc
       4921 ccctctaaac tgagactgag gcctctgcag atgtccaagg aggggatcaa gaaagagtcc
       4981 gctcccgaac tgtctaaaat cattgacaga ccaggatggc tgcccgccgt gaacaagctg
       5041 ttctctgatg gaaatgtcac cgtcgtgcgg agagactctc tgggacgcgt gcgactggag
       5101 agtacagccc acctgcctgt cacttggaag gtgcagtaag aattcggtac cgagctcaag
       5161 cttgcggccg cctatagtgt cacctaaatc gtaacccagc tttcttgtac aaagtggtcg
       5221 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
       5281 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
       5341 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggatgacaa
       5401 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
       5461 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
       5521 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
       5581 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
       5641 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctag
       5701 cataacccct tggggcctct aaacgggtct tgaggggttt tttgtctaga agacaatagc
       5761 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
       5821 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
       5881 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
       5941 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
       6001 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
       6061 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
       6121 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
       6181 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
       6241 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
       6301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
       6361 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
       6421 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
       6481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
       6541 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
       6601 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
       6661 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
       6721 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
       6781 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
       6841 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
       6901 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
       6961 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
       7021 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
       7081 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
       7141 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
       7201 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
       7261 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
       7321 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
       7381 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       7441 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
       7501 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
       7561 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
       7621 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
       7681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
       7741 gtccaaactc atcaatgtat cttatcatgt ctgttcatga agccccttga gcatctgact
       7801 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
       7861 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
       7921 tagagtttgg caacatatgc ccatatgtaa ctagcataac cccttggggc ctctaaacgg
       7981 gtcttgaggg gttttttgct gaaagcatgc ggaggaaatt ctccttgaag tttccctggt
       8041 gttcaaagta aaggagtttg caccagacgc acctctgttc actggtccgg cgtattaaaa
       8101 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
       8161 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
       8221 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
       8281 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
       8341 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
       8401 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
       8461 caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga
       8521 cgtaaacacg ttaaatagag cttggacata tttaacatcg ggcgtgttag ctttattagg
       8581 ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc
       8641 catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt
       8701 tgagcttttt ggaattgcga tcgcataact tcgtatagca tacattatac gaagttataa
       8761 gctcggaacg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
       8821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
       8881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
       8941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
       9001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
       9061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
       9121 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
       9181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
       9241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
       9301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
       9361 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
       9421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
       9481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgttacc aatgcttaat
       9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
       9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
       9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
       9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
       9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
       9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
       9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
       9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
      10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
      10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
      10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
      10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
      10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
      10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgtc
      10441 cgcgcgtt
//

