LOCUS       5._Filifactor_al       11291 bp    DNA     circular     09-APR-2013
DEFINITION  .
ACCESSION   urn.local...1365455717185.7235
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1468..1487
                     /note="Geneious type: motif"
                     /label=H1.3F
     misc_feature    1564..1583
                     /note="Geneious type: motif"
                     /label=pRNA-CMV-F
     misc_feature    1570..1591
                     /note="Geneious type: motif"
                     /label=pEGFP-N-5'
     misc_feature    1615..1633
                     /note="Geneious type: motif"
                     /label=T7 promotor
     misc_feature    1810..5988
                     /label=FialCas9
     misc_feature    complement(6015..6033)
                     /note="Geneious type: motif"
                     /label=SP6
     misc_feature    10805..10824
                     /note="Geneious type: motif"
                     /label=SiRNA5'
ORIGIN      
          1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
         61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
        121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
        181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
        241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
        301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
        361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
        421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
        481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
        541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
        601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
        661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
        721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
        781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
        841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
        901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
        961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
       1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
       1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
       1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
       1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
       1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
       1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
       1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
       1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
       1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
       1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
       1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
       1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
       1861 aaaaagcgca aggtcgaagc gtccatgacc aaggagtatt acctggggct ggatgtgggg
       1921 accaattccg tgggatgggc agtgaccgat tctcagtaca acctgtgcaa gtttaagaaa
       1981 aaggatatgt ggggcatccg gctgttcgaa agcgccaaca cagcaaagga ccggagactg
       2041 cagagaggga ataggcgccg actggagcgg aaaaagcaga gaattgatct gctgcaggaa
       2101 atcttctccc cagagatctg caagattgac cccactttct ttatccgact gaacgaatcc
       2161 cggctgcacc tggaggacaa gtctaacgat ttcaaatacc cactgtttat tgagaaggac
       2221 tattctgata tcgagtacta taaagagttc cccaccattt ttcacctgag gaagcatctg
       2281 atcgagagtg aggaaaaaca ggatatccgg ctgatctacc tggccctgca caacatcatt
       2341 aagacccgag gacattttct gattgacggc gatctgcaga gcgccaagca gctgaggccc
       2401 atcctggata cattcctgct gtccctgcag gaggaacaga acctgtcagt gagcctgtcc
       2461 gaaaatcaga aggacgagta tgaggaaatt ctgaaaaacc gcagcatcgc caagtccgaa
       2521 aaagtgaaaa agctgaagaa tctgtttgag atctcagacg agctggaaaa agaggagaag
       2581 aaggcccaga gcgccgtgat cgagaacttc tgcaagttta tcgtgggaaa taagggcgat
       2641 gtctgtaaat tcctgcgggt gtctaaggag gaactggaga ttgactcttt cagtttttca
       2701 gagggcaagt acgaggacga catcgtgaaa aacctggagg aaaaagtgcc tgaaaaggtc
       2761 tacctgtttg agcagatgaa ggcaatgtat gattggaata ttctggtcga catcctggaa
       2821 accgaggaat acatcagctt cgccaaagtg aagcagtatg agaaacacaa gactaacctg
       2881 cggctgctga gagacatcat tctgaaatac tgcaccaagg atgagtataa tcggatgttt
       2941 aacgacgaga aggaagctgg cagctacacc gcatatgtgg ggaaactgaa aaagaacaac
       3001 aagaagtact ggatcgagaa aaagagaaat cccgaggagt tctacaaatc cctgggcaag
       3061 ctgctggata aaattgagcc tctgaaggag gacctggaag tgctgactat gatgatcgag
       3121 gagtgtaaga accacaccct gctgccaatt cagaaaaata aggacaacgg cgtgatcccc
       3181 caccaggtgc atgaggtcga actgaaaaag atcctggaaa atgccaaaaa gtactattcc
       3241 ttcctgaccg agacagacaa ggatgggtac tcagtggtcc agaaaatcga gagcattttc
       3301 aggtttcgca tcccctacta tgtggggcct ctgagtaccc ggcaccagga aaagggatca
       3361 aacgtgtgga tggtcagaaa acctggcagg gaggatcgca tctacccatg gaatatggag
       3421 gaaatcattg actttgagaa gagcaacgaa aatttcatta cacggatgac taacaaatgt
       3481 acatatctga tcggggaaga tgtcctgccc aagcattctc tgctgtacag taaatatatg
       3541 gtgctgaatg agctgaacaa tgtgaaggtc agaggaaaaa agctgcctac atctctgaaa
       3601 cagaaggtgt tcgaggacct gtttgaaaac aaatccaaag tgactggaaa gaatctgctg
       3661 gagtacctgc agatccagga caaagatatc cagattgacg atctgtctgg cttcgacaag
       3721 gacttcaaga ccagcctgaa gagctatctg gacttcaaaa agcagatttt tggggaggaa
       3781 atcgagaagg aaagcattca gaacatgatc gaagatatca ttaagtggat caccatctac
       3841 ggcaatgaca aggagatgct gaaacgagtg attcgggcta attatagcaa ccagctgaca
       3901 gaggaacaga tgaaaaagat cactggattt cagtacagtg gctgggggaa cttctcaaag
       3961 atgtttctga aagggatcag cggatccgac gtgagcaccg gcgaaacatt cgacatcatt
       4021 accgcaatgt gggagacaga caacaatctg atgcagatcc tgtcaaaaaa gttcaccttt
       4081 atggacaacg tcgaggactt caacagcggc aaggtcggga aaatcgacaa gattacttac
       4141 gatagcaccg tgaaggaaat gttcctgtcc cctgagaaca aaagggccgt ctggcagacc
       4201 attcaggtgg ctgaggagat caagaaagtg atgggctgcg agccaaaaaa gatctttatt
       4261 gaaatggcac ggggcgggga gaaggtgaaa aagaggacaa aatctcgcaa ggcccagctg
       4321 ctggagctgt acgccgcttg cgaggaagat tgtagagaac tgatcaagga gattgaggac
       4381 cgggacgaga gggacttcaa tagcatgaag ctgtttctgt actataccca gttcgggaaa
       4441 tgtatgtatt ccggcgacga catcgatatt aacgagctga ttcgcggcaa ttctaagtgg
       4501 gaccgagatc acatctaccc ccagagcaaa attaaggacg attccatcga taacctggtg
       4561 ctggtcaata agacatataa tgccaaaaag tccaatgagc tgctgtctga ggacatccag
       4621 aaaaagatgc attcattctg gctgagcctg ctgaacaaaa agctgatcac taaaagcaag
       4681 tacgaccgcc tgactcgaaa gggcgacttt accgatgagg aactgagtgg gttcatcgct
       4741 agacagctgg tggaaacaag gcagtcaact aaggcaatcg ccgatatctt caagcagatc
       4801 tacagctccg aggtggtcta tgtgaagagc agcctggtga gcgacttcag gaaaaagcca
       4861 ctgaactacc tgaagtctcg gagagtcaat gattaccacc atgcaaaaga cgcctatctg
       4921 aacattgtgg tcgggaacgt gtacaacaaa aagtttacca gtaatcccat ccagtggatg
       4981 aaaaagaatc gcgatacaaa ctatagcctg aacaaggtgt tcgaacacga cgtggtcatt
       5041 aacggagaag tgatctggga aaagtgcaca taccatgagg acactaatac ctatgatgga
       5101 ggcactctgg accgaatccg gaagattgtg gaacgcgata acattctgta caccgagtac
       5161 gcttattgtg agaagggcga actgtttaat gcaaccatcc agaacaaaaa tggaaactcc
       5221 acagtctctc tgaaaaaggg cctggacgtg aaaaagtacg ggggatactt cagcgccaac
       5281 acaagttact tctcactgat cgagtttgag gacaagaagg gggatagagc aaggcacatc
       5341 attggagtgc ctatctatat tgcaaacatg ctggagcatt ctccaagtgc cttcctggag
       5401 tactgcgaac agaaggggta tcagaatgtg cggattctgg tcgagaaaat caaaaagaac
       5461 agcctgctga tcattaatgg ataccctctg cgcattcgag gcgagaacga agtggatact
       5521 tcctttaaga gggccatcca gctgaagctg gaccagaaaa actatgagct ggtccgcaat
       5581 atcgagaagt tcctggaaaa atacgtggag aaaaagggaa actatccaat tgacgagaat
       5641 agagatcaca tcacacatga aaagatgaac cagctgtacg aggtgctgct gtccaaaatg
       5701 aaaaagttca acaagaaggg catggccgac ccctctgata ggatcgaaaa gagtaagcct
       5761 aaattcatca agctggagga cctgatcgat aagattaatg tgatcaacaa aatgctgaac
       5821 ctgctgcgct gtgacaatga tactaaggcc gacctgtctc tgattgagct gcccaaaaac
       5881 gctgggagtt tcgtggtcaa aaagaatacc atcggaaagt caaaaatcat cctggtgaat
       5941 cagagcgtga ctggactgta cgagaataga cgggaactgt aagaattcgg taccgagctc
       6001 aagcttgcgg ccgcctatag tgtcacctaa atcgtaaccc agctttcttg tacaaagtgg
       6061 tcgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
       6121 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
       6181 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggatga
       6241 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
       6301 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
       6361 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
       6421 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
       6481 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
       6541 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgtct agaagacaat
       6601 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
       6661 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
       6721 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
       6781 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
       6841 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
       6901 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
       6961 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
       7021 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
       7081 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
       7141 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
       7201 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
       7261 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
       7321 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
       7381 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
       7441 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
       7501 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
       7561 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
       7621 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
       7681 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
       7741 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
       7801 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
       7861 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
       7921 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
       7981 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
       8041 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
       8101 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
       8161 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
       8221 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
       8281 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
       8341 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
       8401 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
       8461 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
       8521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
       8581 tttgtccaaa ctcatcaatg tatcttatca tgtctgttca tgaagcccct tgagcatctg
       8641 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
       8701 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
       8761 gtttagagtt tggcaacata tgcccatatg taactagcat aaccccttgg ggcctctaaa
       8821 cgggtcttga ggggtttttt gctgaaagca tgcggaggaa attctccttg aagtttccct
       8881 ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta
       8941 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
       9001 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
       9061 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
       9121 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
       9181 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
       9241 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
       9301 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
       9361 cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt tagctttatt
       9421 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
       9481 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
       9541 agttgagctt tttggaattg cgatcgcata acttcgtata gcatacatta tacgaagtta
       9601 taagctcgga acgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
       9661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
       9721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
       9781 ccgccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
       9841 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
       9901 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
       9961 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      10021 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      10081 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      10141 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      10201 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      10261 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      10321 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgtt accaatgctt
      10381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      10441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      10501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      10561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      10621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      10681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      10741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      10801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      10861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      10921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      10981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      11041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      11101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      11161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      11221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      11281 gtccgcgcgt t
//

