LOCUS       19._Roseburia_in       10616 bp    DNA     circular     09-APR-2013
DEFINITION  .
ACCESSION   urn.local...1365455794187.7279
VERSION
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1468..1487
                     /note="Geneious type: motif"
                     /label=H1.3F
     misc_feature    1564..1583
                     /note="Geneious type: motif"
                     /label=pRNA-CMV-F
     misc_feature    1570..1591
                     /note="Geneious type: motif"
                     /label=pEGFP-N-5'
     misc_feature    1615..1633
                     /note="Geneious type: motif"
                     /label=T7 promotor
     misc_feature    1810..5313
                     /label=RoinCas9
     misc_feature    complement(5340..5358)
                     /note="Geneious type: motif"
                     /label=SP6
     misc_feature    9694..10554
                     /note="Geneious type: motif"
                     /label=AMP
     misc_feature    9694..10554
                     /note="Geneious type: motif"
                     /label=ampr
     misc_feature    10130..10149
                     /note="Geneious type: motif"
                     /label=SiRNA5'
ORIGIN      
          1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
         61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
        121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
        181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
        241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
        301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
        361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
        421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
        481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
        541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
        601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
        661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
        721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
        781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
        841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
        901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
        961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
       1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
       1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
       1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
       1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
       1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
       1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
       1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
       1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
       1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
       1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
       1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
       1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
       1861 aaaaagcgca aggtcgaagc gtccatgaga gagaacggga gtgacgaaag gcggaggaat
       1921 atggacgaaa aaatggatta caggattggg ctggacatcg gcatcgcatc agtggggtgg
       1981 gccgtcctgc agaacaattc agacgatgag cctgtgagga ttgtcgacct gggagtgcgc
       2041 attttcgata ccgcagagat cccaaagaca ggcgaaagcc tggcaggacc ccggagagca
       2101 gctcgaacca caaggcgccg actgcggaga aggaaacacc gcctggaccg aatcaagtgg
       2161 ctgttcgaga accaggggct gatcaatatt gacgattttc tgaagagata caacatggcc
       2221 ggactgccag atgtgtacca gctgcggtat gaggctctgg acagaaaact gaccgatgag
       2281 gaactggccc aggtgctgct gcacatcgct aagcatcggg gcttcagaag caccaggaaa
       2341 gccgagacag cagccaagga aaacggggca gtgctgaagg ccacagacga gaatcagaaa
       2401 cgaatgcagg aaaagggata caggacagtg ggcgagatga tctacctgga cgaggccttc
       2461 cggactggct gctcttggag tgagaaaggg tacatcctga ccccccgcaa caaggctgaa
       2521 aattatcagc acacaatgct gcgggcaatg ctggtggagg aagtcaagga gattttcagc
       2581 tcccagcgcc gactgggcaa cgaaaaagcc actgaggaac tggaggaaaa gtacctggag
       2641 atcatgacct cccagcgctc ttttgacctg gggcctggaa tgcagccaga tgggaagccc
       2701 tccccttatg caatggaggg cttctctgac agagtgggga aatgtacttt tctgggggat
       2761 cagggagagc tgaggggcgc taaggggacc tacacagccg aatatttcgt ggctctgcag
       2821 aaaatcaacc acacaaagct ggtcaatcag gacggcgaga caaggaattt cactgaggaa
       2881 gagcggagag ccctgactct gctgctgttt acccagaaag aggtgaagta cgctgcagtc
       2941 cgcaagaaac tgggcctgcc tgaggacatc ctgttctaca acctgaacta caagaaggcc
       3001 gctactaaag aagagcagca gaaggagaac cagaataccg aaaaagccaa gtttatcggg
       3061 atgccatact atcacgatta caagaaatgc ctggaagaga gagtgaagta tctgaccgag
       3121 aacgaagtca gggacctgtt tgatgagatc ggaatgattc tgacttgtta caaaaatgac
       3181 gattcccgca ccgaacgact ggccaagctg ggactggtgc ccatcgagat ggaaggcctg
       3241 ctggcttata ctcctaccaa attccagcat ctgtctatga aggcaatgcg gaacatcatt
       3301 ccctttctgg agaaagggat gacctacgac aaggcttgcg aagaggcagg atatgacttc
       3361 aaagccgata gcaaggggac taaacagaag ctgctgaccg gagagaacgt gaatcagaca
       3421 atcaacgaaa ttactaatcc tgtggtcaaa cgctcagtga gccagacagt gaaggtcatt
       3481 aacgccatca ttcggactta cggcagtcca caggctatca atattgagct ggcaagagaa
       3541 atgtcaaaga cctttgaaga gaggcgcaaa atcaaggggg acatggagaa acggcagaag
       3601 aacaatgaag atgtgaagaa acagattcag gagctgggaa aactgtctcc tacaggccag
       3661 gacatcctga agtacagact gtggcaggag cagcagggga tttgtatgta tagtggaaaa
       3721 accatcccac tggaagagct gttcaagccc ggctacgaca tcgatcacat tctgccctat
       3781 tcaattacat tcgacgatag ctttaggaac aaagtgctgg tcacatccca ggagaacaga
       3841 cagaagggca ataggactcc ttacgagtat atggggaacg acgaacagcg ctggaatgag
       3901 tttgaaacca gggtgaaaac taccatccgc gattacaaga aacagcagaa gctgctgaag
       3961 aaacatttct ctgaagagga aaggagtgag tttaaagaac ggaacctgac agacactaag
       4021 tacatcacaa ccgtgatcta caacatgatc agacagaatc tggagatggc ccccctgaac
       4081 cgccctgaaa agaaaaagca ggtgcgggct gtcaatggcg caattaccgc ctacctgcga
       4141 aaacggtggg ggctgccaca gaagaatcgg gagacagaca cacaccatgc tatggatgca
       4201 gtggtcatcg cctgctgtac cgacggcatg atccagaaaa ttagtagata cacaaaggtg
       4261 agagagaggt gctattcaaa gggaacagag ttcgtcgatg cagagactgg cgaaatcttt
       4321 agacccgagg actacagcag ggccgagtgg gatgaaattt tcggcgtgca catcccaaag
       4381 ccctgggaga catttcgcgc cgaactggac gtccgaatgg gggacgatcc aaagggattc
       4441 ctggacactc atagcgatgt ggctctggag ctggattatc ccgagtacat ctacgaaaac
       4501 ctgcggccta tcttcgtgag cagaatgcca aatcacaagg tcaccggagc agcccatgct
       4561 gacacaattc ggtccccaag acactttaaa gatgagggca tcgtgctgac taagaccgca
       4621 ctgaccgacc tgaaactgga caaggatggg gagatcgacg gatactataa cccccagtcc
       4681 gatctgctgc tgtacgaagc actgaaaaag cagctgctgc tgtatggcaa tgatgccaaa
       4741 aaggccttcg ctcaggactt tcataaaccc aaggccgatg gaactgaggg ccctgtggtc
       4801 aggaaggtga agatccagaa aaagcagacc atgggagtgt tcgtcgactc tggcaacggg
       4861 attgccgaga atggcgggat ggtgcgcatc gatgtgttcc gagtcaacgg caagtactat
       4921 tttgtgcccg tctacaccgc tgacgtggtc aaaaaggtgc tgcctaatag ggccagtaca
       4981 gctcacaagc catacggcga gtggaaagtg atggaggaca aggatttcct gtttagtctg
       5041 tattcacgcg acctgatcca tatcaagtct aaaaaggata tccctattaa gatggtgaac
       5101 ggaggcatgg aggggatcaa ggaaacctac gcatactata ttggagccga catcagcgct
       5161 gcaaatatcc agggcattgc ccacgattcc aggtataaat tccgcggact gggcattcag
       5221 tctctggacg tgctggagaa gtgtcagatc gatgtgctgg gacatgtcag cgtggtccga
       5281 tccgaaaagc ggatgggctt tagctaagaa ttcggtaccg agctcaagct tgcggccgcc
       5341 tatagtgtca cctaaatcgt aacccagctt tcttgtacaa agtggtcggg tggcatccct
       5401 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
       5461 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
       5521 tggggtggag gggggtggta tggagcaagg ggcaagttgg gatgacaacc tgtagggcct
       5581 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
       5641 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
       5701 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
       5761 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
       5821 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctagca taaccccttg
       5881 gggcctctaa acgggtcttg aggggttttt tgtctagaag acaatagcag gcatgctggg
       5941 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
       6001 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
       6061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
       6121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
       6181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
       6241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
       6301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
       6361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
       6421 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
       6481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
       6541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
       6601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
       6661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
       6721 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
       6781 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
       6841 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
       6901 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
       6961 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
       7021 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
       7081 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
       7141 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
       7201 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
       7261 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
       7321 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
       7381 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
       7441 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
       7501 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
       7561 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
       7621 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
       7681 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
       7741 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
       7801 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
       7861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
       7921 caatgtatct tatcatgtct gttcatgaag ccccttgagc atctgacttc tggctaataa
       7981 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
       8041 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
       8101 acatatgccc atatgtaact agcataaccc cttggggcct ctaaacgggt cttgaggggt
       8161 tttttgctga aagcatgcgg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa
       8221 ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg
       8281 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
       8341 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
       8401 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
       8461 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
       8521 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
       8581 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
       8641 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
       8701 aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
       8761 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
       8821 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
       8881 aattgcgatc gcataacttc gtatagcata cattatacga agttataagc tcggaacgct
       8941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
       9001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
       9061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc ccctgacgag
       9121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
       9181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
       9241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
       9301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
       9361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
       9421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
       9481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
       9541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
       9601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
       9661 cgcagaaaaa aaggatctca agaagatcct ttgttaccaa tgcttaatca gtgaggcacc
       9721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
       9781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
       9841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
       9901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
       9961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      10021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      10081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      10141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      10201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      10261 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      10321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      10381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      10441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      10501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      10561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgtccg cgcgtt
//

