LOCUS       pCM272\pDest/att        7987 bp    DNA     circular     26-OCT-2010
DEFINITION  attB1-attB2-attB3-attB4.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|518098062|
COMMENT     VNTDBDATE|569067835|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCM272 pDest/attB_ubiC2320-EGFP|
COMMENT     VNTAUTHORNAME|Christian Mosimann|
FEATURES             Location/Qualifiers
     misc_feature    6832..6852
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(7642..7662)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    complement(7862..7882)
                     /vntifkey="21"
                     /label=attB3
     misc_feature    3277..3297
                     /vntifkey="21"
                     /label=attB4
     gene            3107..3186
                     /vntifkey="60"
                     /note="white"
     gene            1455..2312
                     /vntifkey="60"
                     /note="Amp"
     misc_signal     7930..227
                     /vntifkey="87"
                     /label=attB
                     /note="attB"
     source          complement(2443..2899)
                     /organism="synthetic construct"
                     /db_xref="taxon:32630 [Split]"
                     /vntifkey="98"
                     /note="mol_type=other DNA"
     misc_feature    complement(2443..2899)
                     /vntifkey="21"
                     /label=Misc_Feature_3
                     /note="phagemid pBluescriptII SK(-) [Split]"
     primer_bind     3241..3262
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13R
     primer_bind     complement(7891..7906)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13F
     CDS             6861..7577
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /vntifkey="4"
                     /label=EGFP
     polyA_signal    7665..7860
                     /ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_revcolor=#ffff7f
                     /vntifkey="25"
                     /label=SV40\late\polyA\(from\pCS2+)
     misc_recomb     7985..7987
                     /vntifkey="86"
                     /label=Crossover\sequence
BASE COUNT     2237 a      1741 c      1750 g      2259 t 
ORIGIN
        1 ggctccccgg gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg 
       61 tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg 
      121 ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg 
      181 cggggcagcg tcagcgggtt ctcgacggtc acggcgggca tgtcgacact agttctagcc 
      241 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg 
      301 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
      361 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 
      421 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 
      481 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
      541 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
      601 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
      661 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
      721 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
      781 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
      841 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
      901 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
      961 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     1021 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     1081 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 
     1141 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     1201 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     1261 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     1321 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     1381 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     1441 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     1501 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     1561 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     1621 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     1681 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     1741 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     1801 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     1861 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     1921 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     1981 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     2041 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     2101 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     2161 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     2221 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     2281 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     2341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     2401 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata 
     2461 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 
     2521 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 
     2581 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 
     2641 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 
     2701 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 
     2761 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 
     2821 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 
     2881 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat 
     2941 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 
     3001 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat 
     3061 tgtaatacga ctcactatag ggcgaattgg gtacgtaccg ggcccctagt atgtatgtaa 
     3121 gttaataaaa cccatttttg cggaaagtag ataaaaaaaa catttttttt ttttactgca 
     3181 ctggatctag cggatccggg aattgggaat tcgttaacag atctgcggcc gcggctcgag 
     3241 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttggct 
     3301 ccgaattcgc ccttaaactc gagaccagca aagttctaga atttgtcgaa acatttatgt 
     3361 tatatatttc ctgaaaaaaa ttctgagtaa gttcttaagt gttattgcca gcaacataaa 
     3421 caacagacgg caaaatgaat aaatgataac aaagcagtag gcttaaataa acctaatttt 
     3481 tataggctgt tctctacaac cctcaaacag tgattagttt tgtacttata aacttgccct 
     3541 ttcattcata tttcaagaaa attggttcag aagatctgga tattctagca gttgttcaag 
     3601 ctcatggagg gatcagtgac ctgattccac aatgactagg cctaatccag aaattagatg 
     3661 actgtcaaca taaaaaggca cagcactcac tagctgccct atatatttta ttatatttta 
     3721 catatattat tttatttatt tagctctgag tgctgtactt tctggttaaa gaaaactgct 
     3781 tacaacagct aacctgtact acctcaggct cagggaattt ggaacaggtt tgtctggttt 
     3841 gtttctttaa ccatgcatgc ttgttttcaa ctatggcaac acagtcacat gggacattac 
     3901 agaaatgatt tgtcgatgac atgcgacttt tctttaataa agcgcaaaga tcccaaaaag 
     3961 caaactttta acaaaaatca tataattata ttttcaatcc agctttgtag caactttgtg 
     4021 ctgctgttca ctcagcaaca gatagtcagt ataaggtcag tgtgtctcaa agcagtgcca 
     4081 tctgtttcac acattgcgtt ctatatataa gtgtgctggt tgacacgaca ctgtataagg 
     4141 cctaggctaa aacacaaaca atgtagaatg acactgtgtt ttttttgtaa acaaatgttg 
     4201 tttttggtta aacatctttg tgaaaacatc ctcctgtcat gtatttgcta tattcaaatg 
     4261 ttaaacccgt gcagaataga acatatacaa aaaaaaacaa cacaacacat ttttaaacat 
     4321 tattaaatat caagtattgc tggcagttct gtttctgttt tacagtaccc tttgccacag 
     4381 ttctccgctt ttcctggtcc agattccaca agtctgattc accaatagca aagcgaataa 
     4441 acaaccaaag cagccaatca ctgcttgtag actgtcctgc gagaccggcc cattccagca 
     4501 cattctggaa acttccttta tatgataatt ataaatacat ttaaattatt gatacaaaac 
     4561 atgtaattcc tagaacataa ccatagcaat cattagtttt cagggtaatt atgtattttt 
     4621 aggatttgac tgcggaaaga tctggtcatg tgacgtctca tgaacgtcac ggccctgggt 
     4681 ttctataaat acagtaggac tctcgaccat cggcagattt ttcgaagaag aagatcagtt 
     4741 tcaggagccg tactgttccg tttcaacgca aatattaacg gtaagagcga atttcctagt 
     4801 ttgttttcat gccattcttt aaaaccatag cgtattactt taattatagt aaactttcgc 
     4861 tttctttatt acaagagacg ttttgtgttg attctccgcg gacattttcg gtcagacaat 
     4921 cagaaaatga ccgcggagga ccagtaactt gcattacacg taagttaaat cttcgtgtat 
     4981 taaaatggtt aggttgttaa cgtcaaatag gttaccgtgt ttgcgtgtga tcaggttggt 
     5041 tttgttagat ttttgtcagt atttttaatt tatttgtttt agtttattta ttttttttgc 
     5101 tgaatcatag tttgtgaaca aagaacccgg atgttacata cagtacagcc gccatgttac 
     5161 agagagttat aacttaatca ttttaaaaat aattttgcct tacttttagt ttgtcatgtt 
     5221 gagaaatgag gaaatgttaa aatgaggaaa tatccaatta atttaatata tcaaaataat 
     5281 ccatgattac aatgcactga actggagaaa attaagatgt tttctagtgt catgaaacaa 
     5341 atgtaagaga tgtacattgt agatgtttta tgtcaagaat tggctagttg atgcagcata 
     5401 ctggcgatac tcagttgtaa taacagtaac gttacatgtt aatagactac tgagtatgct 
     5461 gttctgtcta tgtatgctct gtaagctacg agaaggactt ttttaaacag taaagggtgc 
     5521 aatattttta caaattgaat taaataaagg ctgtctatta agtaatatgc ttgatatttt 
     5581 tcttacttga tcggaaataa gaaaaaatat aaacgttgtt gctctaaaaa tcctagttca 
     5641 gtttagccaa ccacaaatac ctttttgttc ctccaacagt ttttttttct tctctataat 
     5701 atttggcagt ctatagtact ccaaatgttt ccccacagtc taactaattg gtacagccaa 
     5761 aatcatgaca cttattgcaa taataatttt ggttcattgg cattgttgat agcctgtgcc 
     5821 actaatatgg tcgattgatc atgcttcagg aagaaaacta tattgtttga tgtaagatta 
     5881 ttaaatcttc acctgcttcc attacaaact attcccatct tattgaattc tggtatgtct 
     5941 taaaggatta gttcacttcc caaatcaaaa tttacttagc cttttttcat ccatgatccc 
     6001 tttttttcat cattaatgaa gaaattgttt ttgaaaaagt ttcaagattt ttttctctat 
     6061 attgtggagc ttgttagttt aaaattccaa aatgcaatat gtggcttcaa atggttctaa 
     6121 atgatcccag tcaaggaata acagtcttat ctaatgaaac cattagacct ttaaaaataa 
     6181 aaaaaataaa agtatttatt tttaaatgac tgagtgatta agttgaattt cagcgtttcc 
     6241 ttactgtgta gaagtccttc cttactggcc ccaccctttg gttctctgcc aatctgctac 
     6301 ctaatgtaat gttgtggaac attattattc tttatttctt aattttttat tttttatttt 
     6361 aaaacaatgt aaactgcaca gatgtgcagt ttgtttaaaa tggccaatgc tttggaaatg 
     6421 catgacataa ttagatttca tgatgcacaa agccaaatct cagagcttgt gcaaaatgag 
     6481 ctatcatttc actaggtaag accctaaatt ttcatatagg atcatttgga caattttgct 
     6541 gcaggtaaaa tgcattctat agtccactgt cagccattgt tttggatagt atttattttt 
     6601 ctctacaagt atagtcaata gttttctatt attttaaagg tttgtaacat ttaagggtga 
     6661 ccaaatgcaa agtaaaattt cattttcggg tgaactatct cgtttaacat gggagaagtg 
     6721 caaaacatac attattggct agaacattgt agtatttttt aaatggaaat gtgtgattgc 
     6781 taatcttact ttgaatttgt ttacagggat ccaaaaaggg cgaattcgac ccaagtttgt 
     6841 acaaaaaagc aggctggacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 
     6901 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 
     6961 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 
     7021 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 
     7081 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 
     7141 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 
     7201 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 
     7261 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 
     7321 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 
     7381 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 
     7441 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 
     7501 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 
     7561 tggacgagct gtacaagggc ggtggaagat ctgggaattc aaggcctctc gagcctctag 
     7621 attctgcagc cctatagcac ccagctttct tgtacaaagt gggggatcca gacatgataa 
     7681 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 
     7741 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 
     7801 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 
     7861 ccaactttat tatacatagt tgataattca ctggccgtcg ttttacggta cctctagaga 
     7921 tccactagtg tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt 
     7981 gcccttg 
//
