LOCUS       pCM327\pDestCY/a        6636 bp    DNA     circular SYN 12-APR-2010
DEFINITION  Contig[0033], 7418 bases, 6140 checksum.
ACCESSION   -
KEYWORDS    -.
SOURCE      -.
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|551275084|
COMMENT     VNTDBDATE|551275084|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCM327 pDestCY/attB|
COMMENT     VNTAUTHORNAME|Christian Mosimann|
FEATURES             Location/Qualifiers
     gene            667..746
                     /vntifkey="60"
                     /note="white"
     gene            5651..6508
                     /vntifkey="60"
                     /note="Amp"
     misc_signal     4139..4423
                     /vntifkey="87"
                     /label=attB
                     /note="attB"
     frag            3..459
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:frag  1 to 457 of f1 + orientation cass."
     source          complement(<3..>459)
                     /organism="synthetic construct"
                     /db_xref="taxon:32630 [Split]"
                     /vntifkey="98"
                     /note="mol_type=other DNA"
     misc_recomb     3967..4091
                     /ApEinfo_fwdcolor=#5370ff
                     /ApEinfo_revcolor=#5370ff
                     /vntifkey="86"
                     /label=attR3
     misc_recomb     2388..2512
                     /ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_revcolor=#5d40ff
                     /vntifkey="86"
                     /label=attR4
     misc_feature    4071..4085
                     /ApEinfo_fwdcolor=#4259cc
                     /ApEinfo_revcolor=#4259cc
                     /vntifkey="21"
                     /label=att3_shared
     misc_feature    2388..2408
                     /ApEinfo_fwdcolor=#4a33cc
                     /ApEinfo_revcolor=#4a33cc
                     /vntifkey="21"
                     /label=att4_shared
     CDS             complement(3199..3858)
                     /ApEinfo_fwdcolor=#ffbf00
                     /ApEinfo_revcolor=#ffbf00
                     /vntifkey="4"
                     /label=cmR
     misc_feature    complement(2552..2857)
                     /ApEinfo_fwdcolor=#ffbf3f
                     /ApEinfo_revcolor=#ffbf3f
                     /vntifkey="21"
                     /label=ccdB
     primer_bind     2352..2373
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13R
     primer_bind     complement(4100..4115)
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
                     /vntifkey="28"
                     /label=M13F
     promoter        complement(1882..2341)
                     /vntifkey="29"
                     /label=zebrafish\alpha-crystallin\promoter
     5'UTR           complement(1777..1881)
                     /vntifkey="52"
                     /label=5'\UTR
     3'UTR           complement(805..1056)
                     /vntifkey="50"
                     /label=BGH\polyA
     CDS             complement(1057..1776)
                     /vntifkey="4"
                     /label=Venus
BASE COUNT     1656 a      1636 c      1670 g      1674 t 
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg 
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa 
      721 catttttttt ttttactgca ctggatctag cggatccggg aattgggaat tcgttaacag 
      781 atctgcggcc gcggctcgag tacccaccgc ggtggcggcg atctctcccc agcatgcctg 
      841 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa 
      901 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga 
      961 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa 
     1021 ctagaaggca cagtcgaggc tgatcagcgg gccgctttac ttgtacagct cgtccatgcc 
     1081 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt 
     1141 ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg 
     1201 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc 
     1261 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat 
     1321 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc 
     1381 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac 
     1441 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta 
     1501 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc 
     1561 gaagcactgc aggccgtagc ccagggtggt cacgagggtg ggccagggca cgggcagctt 
     1621 gccggtggtg cagatcagct tcagggtcag cttgccgtag gtggcatcgc cctcgccctc 
     1681 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac 
     1741 caccccggtg aacagctcct cgcccttgct caccatgttg tcagacctgg taactcctta 
     1801 ctgtaactta ttgcacctga tgaacaaaag atgtgaactg aacgcaagta ggaggctaat 
     1861 agaggctagt acagtacaaa gatccccaaa tgatgtgtgc cggccttagt ggaggcagac 
     1921 ccctggttat atacctgtgc tgcagaaaga ggggcttaca aatggatctc tctggaatgg 
     1981 cattatctca tggtgggaac agtgttgtca gcagaatcag cggggtggac tctgacctga 
     2041 gaatgacagt ctagcactga atggctcaga caatactctt gtggccaact atcaccggaa 
     2101 aataagtgcc tgccaaaaca aaccagacca gatgaagcta catggctgta gaacgtcagt 
     2161 atttaatgtg ttgtggccac ttggttcacc agatttaaga gctaattttt ttaaatatca 
     2221 cagataatca atcgcttttt ttttttcttg actgaaaagc ttagactgga aaattctttg 
     2281 aaaaagtttg cactacagaa aataaataca taacatcctg cactgaatgc acactattaa 
     2341 tggtactcga gcaggaaaca gctatgacca tgattacgcc aagctatcaa ctttgtatag 
     2401 aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca 
     2461 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggtcgacctg 
     2521 cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc aggttaatgg 
     2581 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga 
     2641 ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca 
     2701 ccgggtaaag ttcacggggg actttatctg acagcagacg tgcactggcc agggggatca 
     2761 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac 
     2821 ggctctctct tttataggtg taaaccttaa actgcatttc accagcccct gttctcgtcg 
     2881 gcaaaggagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc 
     2941 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccgaa 
     3001 ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca 
     3061 ctgtaatacg ctgcttcata gcatacctct ttttgacata cttcgggtat acatatcagt 
     3121 atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttttag 
     3181 taagccggat cctctagatt acgccccgcc ctgccactca tcgcagtact gttgtaattc 
     3241 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag 
     3301 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa 
     3361 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc 
     3421 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta 
     3481 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact 
     3541 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact 
     3601 atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat 
     3661 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt 
     3721 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga 
     3781 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc 
     3841 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg gatcctaact 
     3901 caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg gtgcctaatg 
     3961 cggccgccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg 
     4021 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt caactttatt 
     4081 atacatagtt gataattcac tggccgtcgt tttacggtac ctctagagat ccactagtgt 
     4141 cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct 
     4201 ccccgggcgc gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc 
     4261 ggtagttgat cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg 
     4321 gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg 
     4381 gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc gacactagtt ctagccagct 
     4441 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc 
     4501 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     4561 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 
     4621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 
     4681 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     4741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     4801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     4861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     4921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     4981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     5041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     5101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     5161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     5221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     5281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     5341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     5401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     5461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     5521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     5581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     5641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     5701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     5761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     5821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     5881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     5941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     6001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     6061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     6121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     6181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     6241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     6301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     6361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     6421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     6481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     6541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     6601 taggggttcc gcgcacattt ccccgaaaag tgccac 
//
