LOCUS       pLenti_9xD3B_CLu        9387 bp    DNA     circular UNA 06-JUL-2015
DEFINITION  CRISPR-Display Gaussia Luciferase-2A-Cerulean CFP "reporter"
            vector, lentiviral format.
ACCESSION   urn.local...1435875309127.59
VERSION     urn.local...1435875309127.59
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     gene            complement(1..861)
                     /gene="bla"
                     /label="bla gene"
     CDS             complement(1..861)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="bla CDS"
     sig_peptide     complement(793..861)
                     /gene="bla"
                     /note="required for secretion to the periplasm; cleaved
                     off to form the mature beta-lactamase protein."
                     /label="bla signal peptide"
     -10_signal      complement(905..910)
                     /note="bla promoter (counter-clockwise); GAGACA;
                     transcript start 2548 (complementary strand)"
                     /label="-10 signal"
     -35_signal      complement(926..931)
                     /note="bla promoter (counter-clockwise); TTCAAA"
                     /label="-35 signal"
     source          order(1062..>1473,<8196..1061)
                     /organism="Cloning vector pNEB193"
                     /mol_type="other DNA"
                     /label="source Cloning vector pNEB193"
     primer_bind     1413..1436
                     /created_by="User"
                     /label="M13F(-47) From Genewiz"
     primer_bind     1419..1436
                     /created_by="User"
                     /label="M13(-41)F From Genewiz"
     primer_bind     1439..1456
                     /created_by="User"
                     /label="M13F(-21) From Genewiz"
     Promoter        1483..1892
                     /created_by="User"
                     /label="RSV/5« LTR hybrid Promoter"
     Promoter        1483..1711
                     /created_by="User"
                     /label="RSV Promoter"
     LTR             1712..1892
                     /created_by="User"
                     /label="HIV-1 5« LTR"
     misc_feature    2003..2047
                     /created_by="User"
                     /modified_by="User"
                     /label="HIV-1 psi packaging signal"
                     /note="Geneious type: packaging signal"
     misc_feature    2557..2790
                     /created_by="User"
                     /modified_by="User"
                     /label="HIV-1 Rev responsive element (RRE)"
                     /note="Geneious type: response element"
     misc_feature    3283..3405
                     /created_by="User"
                     /modified_by="User"
                     /label="cPPT"
                     /note="Geneious type: polypyrimidine tract"
     misc_feature    3496..3515
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     misc_feature    3529..3548
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     misc_feature    3567..3586
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     misc_feature    3600..3619
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     misc_feature    3643..3662
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     misc_feature    3676..3695
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     misc_feature    3714..3733
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     misc_feature    3747..3766
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     misc_feature    3780..3799
                     /created_by="User"
                     /label="D3B_gRNA_Binding_Site"
     promoter        3824..3883
                     /gene="minCMV"
                     /modified_by="User"
                     /label="minCMVPro"
     misc_feature    3901..3910
                     /created_by="User"
                     /modified_by="User"
                     /label="Kozak_Sequence"
     sig_peptide     3907..3960
                     /created_by="User"
                     /label="CLuc Signal Peptide"
     CDS             3907..5565
                     /created_by="User"
                     /modified_by="User"
                     /label="Cypridina_Luciferase"
     CDS             5566..5580
                     /created_by="User"
                     /modified_by="User"
                     /label="Linker"
     CDS             5581..5643
                     /created_by="User"
                     /modified_by="User"
                     /label="2A_Self_Cleaving_Peptide"
     CDS             5644..5649
                     /created_by="User"
                     /label="Linker"
     CDS             5650..6366
                     /created_by="User"
                     /modified_by="User"
                     /label="Venus"
     misc_feature    complement(5696..5716)
                     /created_by="User"
                     /modified_by="User"
                     /label="EGFP-N Primer from Genewiz"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6109..6174
                     /created_by="User"
                     /modified_by="User"
                     /label="M153T,V163A,S175G (""Venus"") YFP Variant"
     primer_bind     6303..6324
                     /created_by="User"
                     /label="EGFP Primer from Genewiz"
     CDS             6367..6375
                     /created_by="User"
                     /label="Linker"
     CDS             6376..6432
                     /created_by="User"
                     /label="P2A_Self_Cleaving_Peptide"
     CDS             6433..6438
                     /created_by="User"
                     /label="Linker"
     CDS             6439..7038
                     /created_by="User"
                     /modified_by="User"
                     /label="Puromycin_Resistance_Gene"
     misc_feature    7060..7657
                     /created_by="User"
                     /modified_by="User"
                     /label="WPRE"
     terminator      7758..7992
                     /created_by="User"
                     /modified_by="User"
                     /label="deltaU3/3' LTR"
     misc_feature    7758..7811
                     /created_by="User"
                     /modified_by="User"
                     /label="deltaU3"
     LTR             7812..7992
                     /created_by="User"
                     /label="3' LTR"
     polyA_signal    8064..8195
                     /created_by="User"
                     /label="SV40 PA signal"
     misc_feature    complement(8101..8120)
                     /created_by="User"
                     /label="SV40 pA Primer from Genewiz"
                     /note="Geneious type: primer_bind_reverse"
     -10_signal      complement(8283..8288)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TATGTT"
                     /label="-10 signal"
     -35_signal      complement(8307..8312)
                     /note="Plac promoter (drives lacZalpha;
                     counter-clockwise); TTTACA"
                     /label="-35 signal"
     protein_bind    complement(8332..8344)
                     /note="CAP protein binding site"
                     /bound_moiety="catabolite activator protein (CAP)"
                     /label="CAP protein binding site protein bind"
     rep_origin      complement(8629..9217)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label="rep origin"
     misc_RNA        complement(8629..9181)
                     /note="RNAII transcript (complementary strand)"
                     /label="RNA"
     -35_signal      9035..9040
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label="RNAI promoter (clockwise); TTGAAG -35 signal"
     -10_signal      9057..9062
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label="RNAI promoter (clockwise); GCTACA -10 signal"
     misc_RNA        9071..9178
                     /note="RNAI transcript"
                     /label="RNAI transcript"
     -10_signal      complement(9191..9196)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label="-10 signal"
     -35_signal      complement(9212..9217)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label="-35 signal"
ORIGIN      
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     1081 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     1141 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     1201 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     1261 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     1321 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1381 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1441 taaaacgacg gccagtgaat tcgagctcgg taccttctcg aaaatgtagt cttatgcaat
     1501 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     1561 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     1621 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     1681 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     1741 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     1801 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     1861 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     1921 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     1981 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     2041 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     2101 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     2161 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     2221 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     2281 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     2341 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     2401 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2461 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2521 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2581 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2641 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2701 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2761 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2821 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     2881 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     2941 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3001 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3061 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3121 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3181 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3241 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
     3301 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     3361 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatagggc ccgggcatct
     3421 ggctgggtac acaccccccc atatttctgt gctctgccac ccgtgagctg ccactttccc
     3481 tcgagctttg cggtaatcta gatcgcccgt cccctaggca aagctctaat ctagatcgcc
     3541 cgtcccctag gaaaggaagc agccacatct agatcgcccg tcccctagga taacgttaga
     3601 tctagatcgc ccgtccccta gggtgaagta gtctttgcgg taatctagat cgcccgtccc
     3661 ctaggcaaag ctctaatcta gatcgcccgt cccctaggaa aggaagcagc cacatctaga
     3721 tcgcccgtcc cctaggataa cgttagatct agatcgcccg tcccctaggg tgaagtagta
     3781 tctagatcgc ccgtccccta ggccggatcc acgtatgtcg aggtaggcgt gtacggtggg
     3841 aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggagg taccgccacc
     3901 gccaccatga agaccttaat tcttgccgtt gcattagtct actgcgccac tgttcattgc
     3961 caggactgtc cttacgaacc tgatccacca aacacagttc caacttcctg tgaagctaaa
     4021 gaaggagaat gtattgatag cagctgtggc acctgcacga gagacatact atcagatgga
     4081 ctgtgtgaaa ataaaccagg aaaaacatgt tgccgaatgt gtcagtatgt aattgaatgc
     4141 agagtagagg ccgcaggatg gtttagaaca ttctatggaa agagattcca gttccaggaa
     4201 cctggtacat acgtgttggg tcaaggaacc aagggcggcg actggaaggt gtccatcacc
     4261 ctggagaacc tggatggaac caagggggct gtgctgacca agacaagact ggaagtggct
     4321 ggagacatca ttgacatcgc tcaagctact gagaatccca tcactgtaaa cggtggagct
     4381 gaccctatca tcgccaaccc gtacaccatc ggcgaggtca ccatcgctgt tgttgagatg
     4441 ccaggcttca acatcaccgt cattgagttc ttcaaactga tcgtgatcga catcctcgga
     4501 ggaagatctg taagaatcgc cccagacaca gcaaacaaag gaatgatctc tggcctctgt
     4561 ggagatctta aaatgatgga agatacagac ttcacttcag atccagaaca actcgctatt
     4621 cagcctaaga tcaaccagga gtttgacggt tgtccactct atggaaatcc tgatgacgtt
     4681 gcatactgca aaggtcttct ggagccgtac aaggacagct gccgcaaccc catcaacttc
     4741 tactactaca ccatctcctg cgccttcgcc cgctgtatgg gtggagacga gcgagcctca
     4801 cacgtgctgc ttgactacag ggagacgtgc gctgctcccg aaactagagg aacctgcgtt
     4861 ttgtctggac atactttcta cgatacattt gacaaagcaa gataccaatt ccagggtccc
     4921 tgcaaggaga ttcttatggc cgccgactgt ttctggaaca cttgggatgt gaaggtttca
     4981 cacaggaatg ttgactctta cactgaagta gagaaagtac gaatcaggaa acaatcgact
     5041 gtagtagaac tcattgttga tggaaaacag attctggttg gaggagaagc cgtgtccgtc
     5101 ccgtacagct ctcagaacac ttccatctac tggcaagatg gtgacatact gactacagcc
     5161 atcctacctg aagctctggt ggtcaagttc aacttcaagc aactgctcgt cgtacatatt
     5221 agagatccat tcgatggtaa gacttgcggt atttgcggta actacaacca ggatttcagt
     5281 gatgattctt ttgatgctga aggagcctgt gatctgaccc ccaacccacc gggatgcacc
     5341 gaagaacaga aacctgaagc tgaacgactc tgcaatagtc tcttcgccgg tcaaagtgat
     5401 cttgatcaga aatgtaacgt gtgccacaag cctgaccgtg tcgaacgatg catgtacgag
     5461 tattgcctga ggggacaaca gggtttctgt gaccacgcat gggagttcaa gaaagaatgc
     5521 tacataaagc atggagacac cctagaagta ccagatgaat gcaaaggcag caccagcggc
     5581 ggcagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     5641 ccaggcagta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     5701 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     5761 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     5821 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
     5881 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5941 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     6001 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     6061 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     6121 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     6181 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6241 gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     6301 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6361 tacaagggca gcggtgctac gaacttcagt ctcctgaaac aggctgggga tgtggaggag
     6421 aaccccggcc caggcagcat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6481 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6541 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6601 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6661 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6721 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6781 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6841 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6901 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6961 ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc
     7021 cgcaagcccg gtgcctagta acgtaccggt tagtaatgat cgacaatcaa cctctggatt
     7081 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7141 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7201 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7261 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7321 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7381 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7441 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     7501 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7561 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7621 cgagtcggat ctccctttgg gccgcctccc cgcctggcga tggtaccgct aagcacaatt
     7681 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7741 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7801 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7861 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7921 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7981 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8041 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8101 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8161 tgtccaaact catcaatgta tcttatcatg tctgggttta aacctgcagg catgcaagct
     8221 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8401 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg ccgcttcctc
     8461 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8521 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8581 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8641 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8701 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8761 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8821 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8881 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8941 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9001 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9061 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9121 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9181 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9241 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9301 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9361 tatatatgag taaacttggt ctgacag
//
