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#10# 0) 1 2 0 0 #63# 1 100) COMMENT (LOGPEN 0 8 8388608) 10 1 5.68587 1.9 0.082322 COMMENT 1) COMMENT #123=(CLabel (CWidget 0 (0 0) 1 2 0 0 #55# 0 100) COMMENT (LOGPEN 0 0 4227264) 1 COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.611111 0 "Termination signal" "@N" 1 COMMENT 12.7295 10.454 0 -10 6.01173 0.994066 #122#)) COMMENT (CObjectList)) COMMENT #124=(CGroupWidget COMMENT (CWidget 61 (7 61 0) 1 2 0 0 Nil -480 100) COMMENT (CObjectList COMMENT #125=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #0# 0) 1 2 0 0 #58# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 26367 0) 1 COMMENT 2.36197 3.62391 1 0.082322) 0.8 1.8 0) COMMENT #126=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #55# 1667457383 100) COMMENT (LOGPEN 0 0 4227264) 1 COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.611111 0 "NeoR/KanR" "@N" 1 -5.23067 COMMENT -13.9386 0 -10 3.92893 0.994066 #125#) COMMENT #127=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #58# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 26367 0) 1 COMMENT 1.43972 2.03498 1 0.082322) 0.8 1.8 0) COMMENT #128=(CLabel (CWidget 0 (0 0) 1 2 0 0 #55# 0 100) COMMENT (LOGPEN 0 0 4227264) 1 COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.611111 0 "BleoR" "@N" 1 -14.6448 COMMENT -1.74233 0 -10 2.10647 0.994066 #127#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #129=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -671 100) COMMENT (CObjectList) (CObjectList)) COMMENT #130=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -524 100) COMMENT (CObjectList) (CObjectList)) COMMENT #131=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -598 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) -942357565 COMMENT (CStringList) 3250705607 3351365575 (CObList))) FEATURES Location/Qualifiers exon 1670..2464 /vntifkey="61" /label=NeoR/KanR rep_origin 3183..3771 /vntifkey="33" /label=Ori misc_feature 452..457 /vntifkey="21" /label=BbsI misc_feature 444..449 /vntifkey="21" /label=BbsI primer_bind 239..256 /vntifkey="28" /label=Sp6 primer_bind complement(839..857) /vntifkey="28" /label=T7 promoter 143..173 /vntifkey="30" /label=lac\promoter exon 2671..3045 /vntifkey="61" /label=BleoR promoter complement(459..769) /vntifkey="29" /label=U6\promoter misc_feature complement(378..441) /vntifkey="21" /label=Guide\RNA\scaffold terminator 371..377 /vntifkey="43" /label=Termination\signal BASE COUNT 914 a 1027 c 1071 g 940 t ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 301 ctagtaacgg ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag 361 ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc 421 cttattttaa cttgctattt cacgtcttct cgaagacccg tcctttccac aagatatata 481 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac 541 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta 601 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc 661 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg accttggtac 721 cggatccagt cgactgaatt ggttccttta aagcctgctt ttttgtacaa agggcgaatt 781 ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc ccaattcgcc 841 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa 901 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 961 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat acgtacggca 1021 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 1081 tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 1141 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 1201 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 1261 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 1321 aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc 1381 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag 1441 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct 1501 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg 1561 taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg 1621 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 1681 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 1741 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 1801 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 1861 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 1921 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 1981 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 2041 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 2101 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 2161 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 2221 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 2281 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 2341 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 2401 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 2461 ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg 2521 tatttcacac cgcatacagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 2581 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 2641 cttcaataat agcacgtgag gagggccacc atggccaagt tgaccagtgc cgttccggtg 2701 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc 2761 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc 2821 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc 2881 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc 2941 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac 3001 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctaaaactt 3061 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 3121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 3181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 3241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 3301 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 3361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 3421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 3481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 3541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 3601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 3661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 3721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 3781 aacgcggcct ttttacggtt cctgggcttt tgctggcctt ttgctcacat gttctttcct 3841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag //