LOCUS       NEWMOL                  3952 bp    DNA     circular     21-JUL-2015
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|740068714|
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FEATURES             Location/Qualifiers
     exon            1670..2464
                     /vntifkey="61"
                     /label=NeoR/KanR
     rep_origin      3183..3771
                     /vntifkey="33"
                     /label=Ori
     misc_feature    452..457
                     /vntifkey="21"
                     /label=BbsI
     misc_feature    444..449
                     /vntifkey="21"
                     /label=BbsI
     primer_bind     239..256
                     /vntifkey="28"
                     /label=Sp6
     primer_bind     complement(839..857)
                     /vntifkey="28"
                     /label=T7
     promoter        143..173
                     /vntifkey="30"
                     /label=lac\promoter
     exon            2671..3045
                     /vntifkey="61"
                     /label=BleoR
     promoter        complement(459..769)
                     /vntifkey="29"
                     /label=U6\promoter
     misc_feature    complement(378..441)
                     /vntifkey="21"
                     /label=Guide\RNA\scaffold
     terminator      371..377
                     /vntifkey="43"
                     /label=Termination\signal
BASE COUNT      914 a      1027 c      1071 g       940 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 
      301 ctagtaacgg ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag 
      361 ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc 
      421 cttattttaa cttgctattt cacgtcttct cgaagacccg tcctttccac aagatatata 
      481 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac 
      541 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta 
      601 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc 
      661 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg accttggtac 
      721 cggatccagt cgactgaatt ggttccttta aagcctgctt ttttgtacaa agggcgaatt 
      781 ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc ccaattcgcc 
      841 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa 
      901 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 
      961 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat acgtacggca 
     1021 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 
     1081 tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 
     1141 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 
     1201 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 
     1261 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 
     1321 aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc 
     1381 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag 
     1441 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct 
     1501 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg 
     1561 taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg 
     1621 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 
     1681 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 
     1741 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 
     1801 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 
     1861 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 
     1921 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 
     1981 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 
     2041 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 
     2101 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 
     2161 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 
     2221 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 
     2281 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 
     2341 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 
     2401 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 
     2461 ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg 
     2521 tatttcacac cgcatacagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 
     2581 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 
     2641 cttcaataat agcacgtgag gagggccacc atggccaagt tgaccagtgc cgttccggtg 
     2701 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc 
     2761 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc 
     2821 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc 
     2881 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc 
     2941 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac 
     3001 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctaaaactt 
     3061 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     3121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     3181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     3241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     3301 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 
     3361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     3421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     3481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     3541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     3601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     3661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     3721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     3781 aacgcggcct ttttacggtt cctgggcttt tgctggcctt ttgctcacat gttctttcct 
     3841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 
     3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag 
//
