LOCUS       CAG-cas9-P2A-Cre        9645 bp    DNA     circular      6-MAR-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|691790352|
COMMENT     VNTDBDATE|691790352|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     exon            5960..7033
                     /vntifkey="61"
                     /label=Cre-Myc
     exon            1745..5881
                     /vntifkey="61"
                     /label=cas9\gene
     exon            5888..5953
                     /vntifkey="61"
                     /label=P2A
BASE COUNT     2479 a      2392 c      2606 g      2168 t 
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt 
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 
      181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca 
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 
      361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct 
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 
      481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg 
      541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 
      661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc 
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 
      781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg 
      841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg 
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc 
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg 
     1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg 
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca 
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg 
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc 
     1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 
     1621 gaccggcggc tctagtgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct 
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcggccgc 
     1741 caccatggac aagaagtact ccattgggct cgatatcggc acaaacagcg tcggctgggc 
     1801 cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg gcaataccga 
     1861 tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg gggagacggc 
     1921 cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa agaatcggat 
     1981 ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact ctttcttcca 
     2041 taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc acccaatctt 
     2101 tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat atcatctgag 
     2161 gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc 
     2221 gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc cagacaacag 
     2281 cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt tcgaagagaa 
     2341 cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc 
     2401 ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg gcctgtttgg 
     2461 taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact tcgacctggc 
     2521 cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg acaatctgct 
     2581 ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc tgtcagacgc 
     2641 cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc cgctgagcgc 
     2701 tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga aggcccttgt 
     2761 cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta aaaatggcta 
     2821 cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta ttaagcccat 
     2881 cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag aagatctgtt 
     2941 gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc tgggcgaact 
     3001 gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata acagggaaaa 
     3061 gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg cccggggaaa 
     3121 ttccagattc gcgtggatga ctcgcaaatc agaagagacc atcactccct ggaacttcga 
     3181 ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga ctaactttga 
     3241 taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg agtacttcac 
     3301 agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa agccagcatt 
     3361 cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga accggaaagt 
     3421 taccgtgaaa cagctcaaag aagactattt caaaaagatt gaatgtttcg actctgttga 
     3481 aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg atctcctgaa 
     3541 aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc ttgaggacat 
     3601 tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct tgaaaactta 
     3661 cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat atacaggatg 
     3721 ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg gaaagacaat 
     3781 cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt tgatccatga 
     3841 tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc agggggacag 
     3901 tcttcacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg gaatactgca 
     3961 gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc ccgagaatat 
     4021 cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga acagtaggga 
     4081 aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc ttaaggaaca 
     4141 cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc tgcagaacgg 
     4201 cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact acgacgtgga 
     4261 tcatatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag tgttgacaag 
     4321 atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg tcaagaaaat 
     4381 gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga agttcgataa 
     4441 tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct tcatcaaaag 
     4501 gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg attcacgcat 
     4561 gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta ttactctgaa 
     4621 gtctaagctg gtctcagatt tcagaaagga ctttcagttt tataaggtga gagagatcaa 
     4681 caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg cacttatcaa 
     4741 aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt acgatgttag 
     4801 gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt acttctttta 
     4861 cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag agattcggaa 
     4921 gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca agggtaggga 
     4981 tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta aaaagaccga 
     5041 agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct 
     5101 gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt ctcctacagt 
     5161 cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag 
     5221 cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa aaaaccccat 
     5281 cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc 
     5341 caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga 
     5401 gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct tgtatctggc 
     5461 cagccactat gaaaagctca aagggtctcc cgaagataat gagcagaagc agctgttcgt 
     5521 ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat tctccaaaag 
     5581 agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata agcacaggga 
     5641 taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga ccaacttggg 
     5701 cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt acacctctac 
     5761 aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct atgaaacaag 
     5821 aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga agaggaaggt 
     5881 gaggtccgga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga 
     5941 gaaccctgga cctgctagca tggccaattt actgaccgta caccaaaatt tgcctgcatt 
     6001 accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg 
     6061 ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc 
     6121 atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta 
     6181 tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca 
     6241 gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc 
     6301 actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc 
     6361 tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg 
     6421 ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat 
     6481 agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt 
     6541 aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag 
     6601 cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc 
     6661 gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag 
     6721 ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg 
     6781 cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg 
     6841 agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg 
     6901 ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc 
     6961 aatggtgcgc ctgctggaag atggcgatgg accggtggaa caaaaactta tttctgaaga 
     7021 agatctgtga tagtgtacaa gtaactgcag cgcggggatc tcatgctgga gttcttcgcc 
     7081 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     7141 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     7201 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 
     7261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 
     7321 agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg 
     7381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
     7441 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     7501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     7561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     7621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     7681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     7741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     7801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
     7861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     7921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     7981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     8041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 
     8101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     8161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     8221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     8281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     8341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     8401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     8461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     8521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     8581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     8641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     8701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     8761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     8821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     8881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     8941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     9001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     9061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     9121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     9181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     9241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     9301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     9361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga 
     9421 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc 
     9481 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg 
     9541 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt 
     9601 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatat 
//
