LOCUS       SM112_pCS6-YFP-S        8260 bp    DNA     circular UNA 07-AUG-2015
DEFINITION  pCS6-Venus-deadSMASh.
ACCESSION   urn.local...1425679755235.67
VERSION     urn.local...1425679755235.67
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             1459..2373
                     /label="C ter SMASh tag"
     CDS             2170..2295
                     /note="Geneious type: Degron"
                     /label="Degron"
     misc_feature    736..1452
                     /note="Geneious type: GFP"
                     /label="YFP"
     misc_feature    2296..2337
                     /note="Geneious type: NS4A cofactor portion"
                     /label="NS4A cofactor portion"
     misc_feature    1579..2136
                     /note="Geneious type: Protease"
                     /label="HCV NS3 protease"
     misc_feature    1519..1542
                     /note="Geneious type: affinity tag"
                     /label="FLAG tag"
     misc_feature    6147..7007
                     /note="Geneious type: antibiotics resistance"
                     /label="AmpR"
     misc_feature    order(1747..1749,1993..1995,1819..1821)
                     /note="Geneious type: enzyme"
                     /label="serine protease His-Asp-Ser catalytic triad"
     misc_difference 1738..1740
                     /label="Thr>Ala ""Thr54"" {TSa (slow) - reduces catalytic
                     activity tenfold}"
     primer_bind     668..685
                     /label="SP6 primer"
     promoter        8..534
                     /label="CMV promoter"
     rep_origin      5404..5859
                     /label="M13 origin"
     misc_feature    1993..1995
                     /note="Geneious type: residue"
                     /label="Ser 1165"
     misc_feature    1819..1821
                     /note="Geneious type: residue"
                     /label="Asp 1107"
     misc_feature    1747..1749
                     /note="Geneious type: residue"
                     /label="""His 1083"""
     misc_feature    1459..1485
                     /note="Geneious type: cleavage site"
                     /label="NS4a4b cut site"
     misc_feature    1474..1476
                     /note="Geneious type: cleavage site"
                     /label="P1"
     misc_feature    1459..1461
                     /note="Geneious type: cleavage site"
                     /label="P6"
     misc_feature    complement(1477..1479)
                     /note="Geneious type: cleavage site"
                     /label="P1'"
ORIGIN      
        1 tgcatgtcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
       61 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      121 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      181 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      241 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      301 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      361 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      421 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      481 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
      541 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
      601 ggactctagc ctaggccgcg gagcggataa caatttcaca caggaaacag ctatgaccat
      661 taggcctatt taggtgacac tatagaacaa gtttgtacaa aaaagcaggc tggtaccggt
      721 ccggaattcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      781 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      841 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
      901 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
      961 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1021 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1081 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1141 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     1201 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     1261 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1321 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     1381 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1441 gagctgtaca agcccgggga tgagatggaa gagtgctctc agcacttacc cggcgccggc
     1501 agtagtggcg atatcatgga ttacaaggat gacgacgata agggctcttc cgggacaggc
     1561 tccggatccg gcactagtgc gcccatcacg gcgtacgccc agcagacgag aggcctccta
     1621 gggtgtataa tcaccagcct gactggccgg gacaaaaacc aagtggaggg tgaggtccag
     1681 atcgtgtcaa ctgctaccca aaccttcctg gcaacgtgca tcaatggggt atgctgggca
     1741 gtctaccacg gggccggaac gaggaccatc gcatcaccca agggtcctgt catccagatg
     1801 tataccaatg tggaccaaga ccttgtgggc tggcccgctc ctcaaggttc ccgctcattg
     1861 acaccctgta cctgcggctc ctcggacctt tacctggtca cgaggcacgc cgatgtcatt
     1921 cccgtgcgcc ggcgaggtga tagcaggggt agcctgcttt cgccccggcc catttcctac
     1981 ttgaaaggct cctctggggg tccgctgttg tgccccgcgg gacacgccgt gggcctattc
     2041 agggccgcgg tgtgcacccg tggagtggct aaagcggtgg actttatccc tgtggagaac
     2101 ctagagacaa ccatgagatc cccggtgttc acggacaact cctctccacc agcagtcacc
     2161 ctgacgcacc caatcaccaa aatcgatacc aaatacatca tgacatgcat gtcggccgac
     2221 ctggaggtcg tcacgagcac ctgggtgctc gttggcggcg tcctggctgc tctggccgcg
     2281 tattgcctgt caacaggctg cgtggtcata gtgggcagga tcgtcttgtc cgggaagccg
     2341 gcaattatac ctgacaggga ggttctctac tgaggcgcgc cttcagtagc cagtgacagg
     2401 acccagcctg aatagagggg ccagcccagg gtccccagcc cgcctgccac acccagcctg
     2461 tggcttttgc caactaggac ttgagctggg gctgactccc aaagggatgc cctgtccagc
     2521 cagacatcta ctcacccact gacctggcct gactcacaac tgccacacaa ccatgctaca
     2581 tggactgaac atcaagaagc ccctttccca tagggctccc acctgccgcc tacccctcat
     2641 ctgtctgccc tggtcctggc ccccagcccc attggcctca ccctctacac tctcagacca
     2701 tcacagaaca cgatctggct tcctcattct gctccagtgt ccagggctct ttgggtaatc
     2761 aagaaaccaa gtgtctgaaa ggcaacaaaa agtaggcacc aaaacccagg ggacatccta
     2821 gggcagatgc taaagcagag atgctgaggg aacctcaact tccggggatg cagcccactc
     2881 ctgcagacac agcagatcca gctggtaccc tagctgccgc ccagggcaac cggccagtct
     2941 tgggcagcat agctcccctc ccaggggtga gctgaagcca caaatgcagc tgaagcagca
     3001 gacctgacat cctggcacct cctggccccc aggtgagccc tgcaccgtgt atcttagagt
     3061 gggggggggg ggcgtgcaga accaagtcca ctttccagga gcccttcaag gcttccctag
     3121 caaattagtt gcaagggtgg agtgaagtgg catcagagtg ggtgtcattg tagtgggcac
     3181 tggcacttca cggtccaagg acggggctga ggtgggggcg gttctggggg aatttgctaa
     3241 gccagctcac cgaggaccct gtgatttctc accccagcag tgattcatac cagtgaagaa
     3301 gagcagagct cagctccgtg actcagccat ggcaggcgga gggtccccga ggggctgagt
     3361 cctcacaccc agccgaggca gcggctggag cctacagagc caggagaatg acaccaggta
     3421 agaaagtctt gcctcctatc ctgaccaagc ctcagatccc agccactctc cagatttctg
     3481 ggttatccgg gaagtcctga caggccccag gggtaccagc tgggagcagg gtaacctggt
     3541 gtccctcctg gatctagtgg tgagcagttc tctcaccatc ttgctgcttc ctgtgtcctt
     3601 caggtctcaa ggctgctgag aagtctttgc tgccatgtct ggaaagggca tcaccacacc
     3661 accagcacca gcaccatcct ctcttctcct gaagctgcct acatgggttc caagacactt
     3721 tcaaggcaga gaaaacaaga ttacagagag gaggtgcctg gcagggggca gcaccccagc
     3781 tcagcccaag agctgaaggt gaagacaagc cagcatgaaa ccacgggtct gccatgatgc
     3841 ccgccccgct ggccgctcac tagctgcctg ccattagcct ctgcagcttg agcagggtct
     3901 gtacccttgg tcctcttggc acagagccca gctcgctgca tggcctttgg ctccccgacc
     3961 agacccttgc aggagcctta aggcttgggc ccctgcccag cctgatcttt cctgctgtgc
     4021 cctgcctgcc aggtcaagcc cagtcccagg agaccccagg ccttggctcc taggctgttc
     4081 catgaacctc cctgacctgc ctggtgattg cccagctgaa acctcagcca agccccagct
     4141 ccaattacct tgtgctggta gctgcttgtg tctgcagtct gagtaggcct tgttgcggtt
     4201 cctccatctg cctggtctat tggtgttctg agaccaattc cactgatgtt ctgacagatc
     4261 ctccaccctg tgcccctgcc agcccccaca agtttatttt tgcacaaaag ccatgaccca
     4321 tactcatttg gctggcatag ggtgtgtagg taggccctgg ggactaggga gaccctggag
     4381 atctcaagag agtgtggcta tcccctattt tcaccaagcc ttgaatatcc agccaggctg
     4441 tctgcccata ccatcttacc tcaaagacag atatatatct atatatgatt ttgttaataa
     4501 aactatgaaa cttattggag tatgtgccat ttctcgaggg tgtgggatgg gctgaagcag
     4561 gacctcagag gacactgctc aaggaggtac aggaacaccc accgcctatg taccccagaa
     4621 aaacttctaa gtctagagta tccctcgagg ggcccaagct tacgcgtacc cagctttctt
     4681 gtacaaagtg gtccctatag tgagtcgtat tataagctag gcactggccg tcgttttaca
     4741 acgtcgtgac tgggaaaact gctagcttgg gatctttgtg aaggaacctt acttctgtgg
     4801 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     4861 tttaagtgta taatgtgtta aactagctgc atatgcttgc tgcttgagag ttttgcttac
     4921 tgagtatgat ttatgaaaat attatacaca ggagctagtg attctaattg tttgtgtatt
     4981 ttagattcac agtcccaagg ctcatttcag gcccctcagt cctcacagtc tgttcatgat
     5041 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     5101 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     5161 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5221 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcga
     5281 tcctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggctggcgta
     5341 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5401 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5461 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5521 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5581 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5641 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5701 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5761 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5821 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc aggtggcact tttcggggaa
     5881 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5941 tgccaggtct tggactggtg agaacggctt gctcggcagc ttcgatgtgt gctggaggga
     6001 gaataaaggt ctaagatgtg cgatagaggg aagtcgcatt gaattatgtg ctgtgtaggg
     6061 atcgctggta tcaaatatgt gtgcccaccc ctggcatgag acaataaccc tgataaatgc
     6121 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6901 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7081 tgaagatcct ttttgataat ctcatgccat aacttcgtat aatgtatgct atacgaagtt
     7141 atggcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     7621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7861 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7921 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7981 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8041 gcagagcttg caattcgcgc gtttttcaat attattgaag catttatcag ggttattgtc
     8101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8161 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     8221 ataaaaatag gcgtagtacg aggccctttc actcattaga
//
