LOCUS pMM585 7060 bp DNA circular UNA 08-JUL-2015 DEFINITION pNBU2-LacI-PcfxA-LacO12-NanoLuc. ACCESSION urn.local...6x-4vxo26e VERSION urn.local...6x-4vxo26e KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..98 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="PcfxA" protein_bind 44..64 /created_by="User" /label="LacO1" misc_feature 63..66 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /modified_by="User" /label="-33" protein_bind 67..86 /created_by="User" /label="LacO1'" misc_feature 87..94 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="-7" misc_feature 99 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="+1" gene 142..657 /gene="NanoLuc" /Transferred_From="pD881-SR:145030 PCR Product" /Transferred_Similarity="100.00%" /label="NanoLuc" terminator 701..752 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="Terminator" promoter 813..1121 /created_by="User" /Transferred_From="LacIq Casette PCR Product" /Transferred_Similarity="100.00%" /label="Pbt1311" CDS 1122..2213 /created_by="User" /Transferred_From="LacIq Casette PCR Product" /Transferred_Similarity="100.00%" /label="lacIq" terminator 2220..2280 /created_by="User" /Transferred_From="LacIq Casette PCR Product" /Transferred_Similarity="100.00%" /label="L3S2P21 Terminator" CDS complement(2434..3714) /note="NBU2 integrase" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="NBU2 integrase" misc_recomb 3874..3896 /note="attN2" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="attN2" CDS complement(4154..4888) /note="ermG" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="ermG" CDS 5376..6237 /note="beta-lactamase from pBR322" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="beta-lactamase from pBR322" misc_recomb 6275..7060 /note="RP4 oriT/RSK oriV" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="RP4 oriT/RSK oriV" ORIGIN 1 ttacaaagaa aattcgacaa actgttattt ttctatctat ttaaattgtg agcggataac 61 aatttgaatt gtgagcggat aacaattacc tttgtcggca aataaagata ttctcgtcaa 121 acaaatataa ataatataaa catggttttt actctggaag attttgttgg cgattggcgt 181 cagaccgcgg gttataattt ggatcaagtc ctggaacagg gtggcgtaag ctctctgttc 241 cagaacctgg gtgtgagcgt gacgccgatt cagcgcatcg ttctgtccgg cgagaacggt 301 ctgaaaattg atattcatgt gatcatcccg tacgaaggcc tgagcggtga ccaaatgggt 361 caaatcgaga aaatctttaa agtcgtctac ccagttgacg atcaccactt caaggttatc 421 ttgcattacg gtacgctggt gattgatggt gtgaccccga atatgattga ctatttcggc 481 cgtccgtatg aaggcattgc cgtttttgac ggtaaaaaga tcaccgtcac cggtaccctg 541 tggaatggca ataagattat tgacgagcgt ctgattaacc cggacggcag cctgctgttc 601 cgcgtgacca tcaacggtgt cacgggttgg cgtctgtgcg agcgcatcct ggcataatga 661 actgcacttg ctttgataat taatgataaa caatctaaaa gcactctaat cgttatcgga 721 gtgcttttag attactaatc aaattgcttc tactaattgc ctatcttcca gtgatggaac 781 agcatttgtg cattggctgc aacaatcagc cttgatctgg aagaagcaat gaaagctgct 841 gttaagtctc cgaatcaggt attgttcctg acaggtgtat tcccatccgg taaacgcgga 901 tactttgcag ttgatctgac tcaggaataa attataaatt aaggtaagaa gattgtagga 961 taagctaatg aaatagaaaa aggatgccgt cacacaactt gtcggcattc ttttttgttt 1021 tattagttga aaatatagtg aaaaagttgc ctaaatatgt atgttaacaa attatttgtc 1081 gtaactttgc actccaaatc tgtttttaac atatggcact agtggtgaat gtgaaaccag 1141 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 1201 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 1261 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 1321 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 1381 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 1441 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 1501 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 1561 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 1621 aggacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 1681 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 1741 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 1801 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 1861 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 1921 ttggtgcgga tatctcggta gtgggatacg acgataccga ggacagctca tgttatatcc 1981 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 2041 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 2101 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 2161 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagctttcc 2221 tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc 2281 tacttgtgcc tgttctattt ccgaaccgac cgcttgtatg aatccatcaa aattcgtttt 2341 ctctatgttg gattccttgt tgctcatatt gtgatgataa tttctacaaa tatagtcatt 2401 ggtaactatc tatgaaactg tttgatactt ttatagttga ttaaacttgt tcatggcatt 2461 tgccttaata tcatccgcta tgtcaatgta gggtttcata gctttgtagt cgctgtgtcc 2521 cgtccatttc atgaccacct gtgccgggat tccgagagcc agcgcattgc agatgaatgt 2581 ccttcttcct gcatgggtac tgagcaaagc gtatttgggt gtgacttcat caatacgttc 2641 atttcccttg tagtaggttt cccgtacagg ctcgttgatt tctgccagtt cgcccagctc 2701 tttcaggtaa tcgttcatct tctggttgct gatgacgggc agagccatgt aattctcgaa 2761 atggatgtcc ttgtatttgt ccagtatggc tttgctgtat ttgttcagtt caatcgtcag 2821 gctgtcggca gtcttgactg tggttatttc gatgtggtcg gacttcacat cgcttctttt 2881 cagattgcga acatccgaat accgcaaact cgtaaagcag cagaacagga aaacatcacg 2941 cacacgttcc aggtattgct tatccttggg tatctggtag tctttcagct tgttcagttc 3001 atcccaagtc aggaagatta cttttttcga ggtggttttc agtttcggtt tgaacgtatc 3061 gtatgcaatg ttctgatgat gtcctttctt gaagctccag cgcaggaacc atttgaggaa 3121 tcccatttgc ttgccgatgg tgctgtttct catatccttg gtgtcacgca ggaagttgac 3181 gtattcgttc aatccaaact cgttgaaata gttgaacgtt gcatcctcct tgaactcttt 3241 gaggtggttc ctcactgctg caaatttttc ataggtggat gccgtccagt tattctggtt 3301 accgcactct tttacaaact catcgaacac ctcccaaaag ctgacagggg cttcttccgg 3361 ctgttcttcg ctggtgtctt tcattctcat gttgaaagct tccttcaact gttgggtcgt 3421 tggcatgacc tcctgcacct caaattcctt gaaaatattc tggatttcgg catagtattt 3481 cagcaagtcc gtattgattt cggctgcact ttgctttagc ttgttggtac atccgctctt 3541 tacccgctgc ttatctgcat cccatttggc tacgtcaatc cggtagcccg ttgtaaactc 3601 gatgcgttgg ctggcaaaga tgacacgcat acggatgggt acgttctcta cgattggcac 3661 accgttcttt ttccggctct ccaatgcaaa aatgatgttg cgcttgatat tcataattgg 3721 gtgcgtttga aattctacac ccaaatatac acccaattat tgagatagca aaagacattt 3781 agaaacattt acttttactc tatattgtaa tttacacttg attatcagtc gtttgcagtc 3841 ttatgatatt ctgtgaaagt ataagttcga gagcctgtct ctccgcaaaa aacgctgaaa 3901 atcagcagat tgcaaaacaa acaccctgtt ttacacccaa gaatgtaaag tcggctgttt 3961 ttgttttatt taagataata caaccactac ataataaaag agtagcgata ttaaaagaat 4021 ccgatgagaa aagactaata tttatctatc cattcagttt gatttttcag gactttacat 4081 cgtcctgaaa gtatttgttg gtaccggtac cgaggacgcg taaacattta cagttgcatg 4141 tggcctattg tttttagccg ttaaatattt tataactatt aaatagcgat acaaattgtt 4201 cgaaactaat attgtttata tcatatattc tcgcatgttt taaagcttta ttaaattgat 4261 tttttgtaaa cagtttttcg tactctttgt taacccattt cattacaaaa gtttcatatt 4321 tttttctctc tttaaatgcc atttttgctg gctttctttt taatacaatt aatgtgctat 4381 ccactttagg ttttggatgg aaataatacc taggaatttt tgctaatata gaaatatcta 4441 cctctgccat taacagcaat gctagtgatc tgtttgtatc taataacatt ttagcaaaac 4501 catattccac tattaaataa cttattgtgg ctgaactttc aaaaacaatt tttcgaatta 4561 tatttgtgct tatgttgtaa ggtatgctgc caaatatttt atatggattg tggctaggaa 4621 atgtaaattt cagtatatca tcatttacta tttgatagtt aggataattt aagagcttat 4681 tacgagttac ctcacataat ttagaatcaa tttctatcgc cgttacaaaa ttacatctct 4741 ttaccaatcc agcagtaaaa tgacctttcc ctgcacctat ttcaaagatg ttatcttttt 4801 catctaaact tatgcaattc attatttttt ctatgtgata ttttgaagta ataaaatttt 4861 gactatcttt tatatttact ttgttcatta taacctctcc ttaatttatt gcatctcttt 4921 tcgaatattt atgttttttg agaaaagaac gtactcatgg ttcatcccga tatgcgtatc 4981 ggtctgtata tcagcaactt tctatgtgtt tcaactacaa tagtcatcta ttctcatctt 5041 tctgagtcca ccccctgcaa agcccctctt tacgacataa aaattcggtc ggaaaaggta 5101 tgcaaaagat gtttctctct ttaagagaaa ctcttcggga tgcaaaaata tgaaaataac 5161 tccaattcac caaattatat agcgactttt ttacaaaatg ctaaaatttg ttgatttccg 5221 tcaagcaatt gttgagcaaa aatgtctttt acgataaaat gatacctcaa tatcaactgt 5281 ttagcaaaac gatatttctc ttaaagagag aaacaccttt ttgttcacca atccccgact 5341 tttaatcccg cggccatgat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 5401 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 5461 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 5521 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 5581 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 5641 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 5701 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 5761 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 5821 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 5881 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 5941 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 6001 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 6061 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 6121 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 6181 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 6241 cagaccaagt ttactcataa cgcgtcaatt cgagggggat caattccgtg ataggtgggc 6301 tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg 6361 cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag 6421 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct 6481 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc 6541 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg 6601 cagcggaaaa cggaattgat ccggccacga tgcgtccggc gtagaggatc tgaagatcag 6661 cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg 6721 tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac gtactaagct 6781 ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat 6841 aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa 6901 aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg 6961 gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac 7021 acttaacggc tgacatggga attcccctcc accgcggtgg //