LOCUS       pMM585                  7060 bp    DNA     circular UNA 08-JUL-2015
DEFINITION  pNBU2-LacI-PcfxA-LacO12-NanoLuc.
ACCESSION   urn.local...6x-4vxo26e
VERSION     urn.local...6x-4vxo26e
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..98
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="PcfxA"
     protein_bind    44..64
                     /created_by="User"
                     /label="LacO1"
     misc_feature    63..66
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="-33"
     protein_bind    67..86
                     /created_by="User"
                     /label="LacO1'"
     misc_feature    87..94
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="-7"
     misc_feature    99
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="+1"
     gene            142..657
                     /gene="NanoLuc"
                     /Transferred_From="pD881-SR:145030 PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="NanoLuc"
     terminator      701..752
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="Terminator"
     promoter        813..1121
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="Pbt1311"
     CDS             1122..2213
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="lacIq"
     terminator      2220..2280
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="L3S2P21 Terminator"
     CDS             complement(2434..3714)
                     /note="NBU2 integrase"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="NBU2 integrase"
     misc_recomb     3874..3896
                     /note="attN2"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="attN2"
     CDS             complement(4154..4888)
                     /note="ermG"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="ermG"
     CDS             5376..6237
                     /note="beta-lactamase from pBR322"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="beta-lactamase from pBR322"
     misc_recomb     6275..7060
                     /note="RP4 oriT/RSK oriV"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="RP4 oriT/RSK oriV"
ORIGIN      
        1 ttacaaagaa aattcgacaa actgttattt ttctatctat ttaaattgtg agcggataac
       61 aatttgaatt gtgagcggat aacaattacc tttgtcggca aataaagata ttctcgtcaa
      121 acaaatataa ataatataaa catggttttt actctggaag attttgttgg cgattggcgt
      181 cagaccgcgg gttataattt ggatcaagtc ctggaacagg gtggcgtaag ctctctgttc
      241 cagaacctgg gtgtgagcgt gacgccgatt cagcgcatcg ttctgtccgg cgagaacggt
      301 ctgaaaattg atattcatgt gatcatcccg tacgaaggcc tgagcggtga ccaaatgggt
      361 caaatcgaga aaatctttaa agtcgtctac ccagttgacg atcaccactt caaggttatc
      421 ttgcattacg gtacgctggt gattgatggt gtgaccccga atatgattga ctatttcggc
      481 cgtccgtatg aaggcattgc cgtttttgac ggtaaaaaga tcaccgtcac cggtaccctg
      541 tggaatggca ataagattat tgacgagcgt ctgattaacc cggacggcag cctgctgttc
      601 cgcgtgacca tcaacggtgt cacgggttgg cgtctgtgcg agcgcatcct ggcataatga
      661 actgcacttg ctttgataat taatgataaa caatctaaaa gcactctaat cgttatcgga
      721 gtgcttttag attactaatc aaattgcttc tactaattgc ctatcttcca gtgatggaac
      781 agcatttgtg cattggctgc aacaatcagc cttgatctgg aagaagcaat gaaagctgct
      841 gttaagtctc cgaatcaggt attgttcctg acaggtgtat tcccatccgg taaacgcgga
      901 tactttgcag ttgatctgac tcaggaataa attataaatt aaggtaagaa gattgtagga
      961 taagctaatg aaatagaaaa aggatgccgt cacacaactt gtcggcattc ttttttgttt
     1021 tattagttga aaatatagtg aaaaagttgc ctaaatatgt atgttaacaa attatttgtc
     1081 gtaactttgc actccaaatc tgtttttaac atatggcact agtggtgaat gtgaaaccag
     1141 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     1201 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     1261 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     1321 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     1381 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     1441 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     1501 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     1561 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     1621 aggacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     1681 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     1741 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     1801 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     1861 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     1921 ttggtgcgga tatctcggta gtgggatacg acgataccga ggacagctca tgttatatcc
     1981 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     2041 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     2101 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2161 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagctttcc
     2221 tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc
     2281 tacttgtgcc tgttctattt ccgaaccgac cgcttgtatg aatccatcaa aattcgtttt
     2341 ctctatgttg gattccttgt tgctcatatt gtgatgataa tttctacaaa tatagtcatt
     2401 ggtaactatc tatgaaactg tttgatactt ttatagttga ttaaacttgt tcatggcatt
     2461 tgccttaata tcatccgcta tgtcaatgta gggtttcata gctttgtagt cgctgtgtcc
     2521 cgtccatttc atgaccacct gtgccgggat tccgagagcc agcgcattgc agatgaatgt
     2581 ccttcttcct gcatgggtac tgagcaaagc gtatttgggt gtgacttcat caatacgttc
     2641 atttcccttg tagtaggttt cccgtacagg ctcgttgatt tctgccagtt cgcccagctc
     2701 tttcaggtaa tcgttcatct tctggttgct gatgacgggc agagccatgt aattctcgaa
     2761 atggatgtcc ttgtatttgt ccagtatggc tttgctgtat ttgttcagtt caatcgtcag
     2821 gctgtcggca gtcttgactg tggttatttc gatgtggtcg gacttcacat cgcttctttt
     2881 cagattgcga acatccgaat accgcaaact cgtaaagcag cagaacagga aaacatcacg
     2941 cacacgttcc aggtattgct tatccttggg tatctggtag tctttcagct tgttcagttc
     3001 atcccaagtc aggaagatta cttttttcga ggtggttttc agtttcggtt tgaacgtatc
     3061 gtatgcaatg ttctgatgat gtcctttctt gaagctccag cgcaggaacc atttgaggaa
     3121 tcccatttgc ttgccgatgg tgctgtttct catatccttg gtgtcacgca ggaagttgac
     3181 gtattcgttc aatccaaact cgttgaaata gttgaacgtt gcatcctcct tgaactcttt
     3241 gaggtggttc ctcactgctg caaatttttc ataggtggat gccgtccagt tattctggtt
     3301 accgcactct tttacaaact catcgaacac ctcccaaaag ctgacagggg cttcttccgg
     3361 ctgttcttcg ctggtgtctt tcattctcat gttgaaagct tccttcaact gttgggtcgt
     3421 tggcatgacc tcctgcacct caaattcctt gaaaatattc tggatttcgg catagtattt
     3481 cagcaagtcc gtattgattt cggctgcact ttgctttagc ttgttggtac atccgctctt
     3541 tacccgctgc ttatctgcat cccatttggc tacgtcaatc cggtagcccg ttgtaaactc
     3601 gatgcgttgg ctggcaaaga tgacacgcat acggatgggt acgttctcta cgattggcac
     3661 accgttcttt ttccggctct ccaatgcaaa aatgatgttg cgcttgatat tcataattgg
     3721 gtgcgtttga aattctacac ccaaatatac acccaattat tgagatagca aaagacattt
     3781 agaaacattt acttttactc tatattgtaa tttacacttg attatcagtc gtttgcagtc
     3841 ttatgatatt ctgtgaaagt ataagttcga gagcctgtct ctccgcaaaa aacgctgaaa
     3901 atcagcagat tgcaaaacaa acaccctgtt ttacacccaa gaatgtaaag tcggctgttt
     3961 ttgttttatt taagataata caaccactac ataataaaag agtagcgata ttaaaagaat
     4021 ccgatgagaa aagactaata tttatctatc cattcagttt gatttttcag gactttacat
     4081 cgtcctgaaa gtatttgttg gtaccggtac cgaggacgcg taaacattta cagttgcatg
     4141 tggcctattg tttttagccg ttaaatattt tataactatt aaatagcgat acaaattgtt
     4201 cgaaactaat attgtttata tcatatattc tcgcatgttt taaagcttta ttaaattgat
     4261 tttttgtaaa cagtttttcg tactctttgt taacccattt cattacaaaa gtttcatatt
     4321 tttttctctc tttaaatgcc atttttgctg gctttctttt taatacaatt aatgtgctat
     4381 ccactttagg ttttggatgg aaataatacc taggaatttt tgctaatata gaaatatcta
     4441 cctctgccat taacagcaat gctagtgatc tgtttgtatc taataacatt ttagcaaaac
     4501 catattccac tattaaataa cttattgtgg ctgaactttc aaaaacaatt tttcgaatta
     4561 tatttgtgct tatgttgtaa ggtatgctgc caaatatttt atatggattg tggctaggaa
     4621 atgtaaattt cagtatatca tcatttacta tttgatagtt aggataattt aagagcttat
     4681 tacgagttac ctcacataat ttagaatcaa tttctatcgc cgttacaaaa ttacatctct
     4741 ttaccaatcc agcagtaaaa tgacctttcc ctgcacctat ttcaaagatg ttatcttttt
     4801 catctaaact tatgcaattc attatttttt ctatgtgata ttttgaagta ataaaatttt
     4861 gactatcttt tatatttact ttgttcatta taacctctcc ttaatttatt gcatctcttt
     4921 tcgaatattt atgttttttg agaaaagaac gtactcatgg ttcatcccga tatgcgtatc
     4981 ggtctgtata tcagcaactt tctatgtgtt tcaactacaa tagtcatcta ttctcatctt
     5041 tctgagtcca ccccctgcaa agcccctctt tacgacataa aaattcggtc ggaaaaggta
     5101 tgcaaaagat gtttctctct ttaagagaaa ctcttcggga tgcaaaaata tgaaaataac
     5161 tccaattcac caaattatat agcgactttt ttacaaaatg ctaaaatttg ttgatttccg
     5221 tcaagcaatt gttgagcaaa aatgtctttt acgataaaat gatacctcaa tatcaactgt
     5281 ttagcaaaac gatatttctc ttaaagagag aaacaccttt ttgttcacca atccccgact
     5341 tttaatcccg cggccatgat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5401 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5461 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5521 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5581 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5641 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5701 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5761 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5821 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5881 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5941 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6001 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6061 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6121 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6181 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6241 cagaccaagt ttactcataa cgcgtcaatt cgagggggat caattccgtg ataggtgggc
     6301 tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg
     6361 cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
     6421 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
     6481 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
     6541 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
     6601 cagcggaaaa cggaattgat ccggccacga tgcgtccggc gtagaggatc tgaagatcag
     6661 cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg
     6721 tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac gtactaagct
     6781 ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat
     6841 aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa
     6901 aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg
     6961 gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac
     7021 acttaacggc tgacatggga attcccctcc accgcggtgg
//
