LOCUS pMM596 7081 bp DNA circular UNA 08-JUL-2015 DEFINITION pNBU2-LacI-PcfxA-LacO13-NanoLuc. ACCESSION urn.local...77-4vxo27y VERSION urn.local...77-4vxo27y KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers promoter 1..98 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="PcfxA" protein_bind 44..64 /created_by="User" /label="LacO1" misc_feature 63..66 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /modified_by="User" /label="-33" misc_feature 87..94 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="-7" misc_feature 99 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="+1" protein_bind 100..120 /created_by="User" /label="LacO1" gene 163..678 /gene="NanoLuc" /Transferred_From="pD881-SR:145030 PCR Product" /Transferred_Similarity="100.00%" /label="NanoLuc" terminator 722..773 /created_by="User" /X-Transferred_From="PcfxA-sfGFP PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="Terminator" promoter 834..1142 /created_by="User" /Transferred_From="LacIq Casette PCR Product" /Transferred_Similarity="100.00%" /label="Pbt1311" CDS 1143..2234 /created_by="User" /Transferred_From="LacIq Casette PCR Product" /Transferred_Similarity="100.00%" /label="lacIq" terminator 2241..2301 /created_by="User" /Transferred_From="LacIq Casette PCR Product" /Transferred_Similarity="100.00%" /label="L3S2P21 Terminator" CDS complement(2455..3735) /note="NBU2 integrase" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="NBU2 integrase" misc_recomb 3895..3917 /note="attN2" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="attN2" CDS complement(4175..4909) /note="ermG" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="ermG" CDS 5397..6258 /note="beta-lactamase from pBR322" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="beta-lactamase from pBR322" misc_recomb 6296..7081 /note="RP4 oriT/RSK oriV" /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR Product" /X-Transferred_Similarity="100.00%" /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product" /Transferred_Similarity="100.00%" /label="RP4 oriT/RSK oriV" ORIGIN 1 ttacaaagaa aattcgacaa actgttattt ttctatctat ttaaattgtg agcggataac 61 aatttgggtg ggaaacttta gttatgtacc tttgtcggca attgtgagcg gataacaatt 121 aaataaagat attctcgtca aacaaatata aataatataa acatggtttt tactctggaa 181 gattttgttg gcgattggcg tcagaccgcg ggttataatt tggatcaagt cctggaacag 241 ggtggcgtaa gctctctgtt ccagaacctg ggtgtgagcg tgacgccgat tcagcgcatc 301 gttctgtccg gcgagaacgg tctgaaaatt gatattcatg tgatcatccc gtacgaaggc 361 ctgagcggtg accaaatggg tcaaatcgag aaaatcttta aagtcgtcta cccagttgac 421 gatcaccact tcaaggttat cttgcattac ggtacgctgg tgattgatgg tgtgaccccg 481 aatatgattg actatttcgg ccgtccgtat gaaggcattg ccgtttttga cggtaaaaag 541 atcaccgtca ccggtaccct gtggaatggc aataagatta ttgacgagcg tctgattaac 601 ccggacggca gcctgctgtt ccgcgtgacc atcaacggtg tcacgggttg gcgtctgtgc 661 gagcgcatcc tggcataatg aactgcactt gctttgataa ttaatgataa acaatctaaa 721 agcactctaa tcgttatcgg agtgctttta gattactaat caaattgctt ctactaattg 781 cctatcttcc agtgatggaa cagcatttgt gcattggctg caacaatcag ccttgatctg 841 gaagaagcaa tgaaagctgc tgttaagtct ccgaatcagg tattgttcct gacaggtgta 901 ttcccatccg gtaaacgcgg atactttgca gttgatctga ctcaggaata aattataaat 961 taaggtaaga agattgtagg ataagctaat gaaatagaaa aaggatgccg tcacacaact 1021 tgtcggcatt cttttttgtt ttattagttg aaaatatagt gaaaaagttg cctaaatatg 1081 tatgttaaca aattatttgt cgtaactttg cactccaaat ctgtttttaa catatggcac 1141 tagtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 1201 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 1261 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 1321 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 1381 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 1441 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 1501 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 1561 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 1621 acagtattat tttctcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat 1681 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 1741 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 1801 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 1861 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 1921 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg 1981 aggacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 2041 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 2101 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 2161 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 2221 aaagcgggca gtgagctttc ctcggtacca aattccagaa aagaggcctc ccgaaagggg 2281 ggcctttttt cgttttggtc ctacttgtgc ctgttctatt tccgaaccga ccgcttgtat 2341 gaatccatca aaattcgttt tctctatgtt ggattccttg ttgctcatat tgtgatgata 2401 atttctacaa atatagtcat tggtaactat ctatgaaact gtttgatact tttatagttg 2461 attaaacttg ttcatggcat ttgccttaat atcatccgct atgtcaatgt agggtttcat 2521 agctttgtag tcgctgtgtc ccgtccattt catgaccacc tgtgccggga ttccgagagc 2581 cagcgcattg cagatgaatg tccttcttcc tgcatgggta ctgagcaaag cgtatttggg 2641 tgtgacttca tcaatacgtt catttccctt gtagtaggtt tcccgtacag gctcgttgat 2701 ttctgccagt tcgcccagct ctttcaggta atcgttcatc ttctggttgc tgatgacggg 2761 cagagccatg taattctcga aatggatgtc cttgtatttg tccagtatgg ctttgctgta 2821 tttgttcagt tcaatcgtca ggctgtcggc agtcttgact gtggttattt cgatgtggtc 2881 ggacttcaca tcgcttcttt tcagattgcg aacatccgaa taccgcaaac tcgtaaagca 2941 gcagaacagg aaaacatcac gcacacgttc caggtattgc ttatccttgg gtatctggta 3001 gtctttcagc ttgttcagtt catcccaagt caggaagatt acttttttcg aggtggtttt 3061 cagtttcggt ttgaacgtat cgtatgcaat gttctgatga tgtcctttct tgaagctcca 3121 gcgcaggaac catttgagga atcccatttg cttgccgatg gtgctgtttc tcatatcctt 3181 ggtgtcacgc aggaagttga cgtattcgtt caatccaaac tcgttgaaat agttgaacgt 3241 tgcatcctcc ttgaactctt tgaggtggtt cctcactgct gcaaattttt cataggtgga 3301 tgccgtccag ttattctggt taccgcactc ttttacaaac tcatcgaaca cctcccaaaa 3361 gctgacaggg gcttcttccg gctgttcttc gctggtgtct ttcattctca tgttgaaagc 3421 ttccttcaac tgttgggtcg ttggcatgac ctcctgcacc tcaaattcct tgaaaatatt 3481 ctggatttcg gcatagtatt tcagcaagtc cgtattgatt tcggctgcac tttgctttag 3541 cttgttggta catccgctct ttacccgctg cttatctgca tcccatttgg ctacgtcaat 3601 ccggtagccc gttgtaaact cgatgcgttg gctggcaaag atgacacgca tacggatggg 3661 tacgttctct acgattggca caccgttctt tttccggctc tccaatgcaa aaatgatgtt 3721 gcgcttgata ttcataattg ggtgcgtttg aaattctaca cccaaatata cacccaatta 3781 ttgagatagc aaaagacatt tagaaacatt tacttttact ctatattgta atttacactt 3841 gattatcagt cgtttgcagt cttatgatat tctgtgaaag tataagttcg agagcctgtc 3901 tctccgcaaa aaacgctgaa aatcagcaga ttgcaaaaca aacaccctgt tttacaccca 3961 agaatgtaaa gtcggctgtt tttgttttat ttaagataat acaaccacta cataataaaa 4021 gagtagcgat attaaaagaa tccgatgaga aaagactaat atttatctat ccattcagtt 4081 tgatttttca ggactttaca tcgtcctgaa agtatttgtt ggtaccggta ccgaggacgc 4141 gtaaacattt acagttgcat gtggcctatt gtttttagcc gttaaatatt ttataactat 4201 taaatagcga tacaaattgt tcgaaactaa tattgtttat atcatatatt ctcgcatgtt 4261 ttaaagcttt attaaattga ttttttgtaa acagtttttc gtactctttg ttaacccatt 4321 tcattacaaa agtttcatat ttttttctct ctttaaatgc catttttgct ggctttcttt 4381 ttaatacaat taatgtgcta tccactttag gttttggatg gaaataatac ctaggaattt 4441 ttgctaatat agaaatatct acctctgcca ttaacagcaa tgctagtgat ctgtttgtat 4501 ctaataacat tttagcaaaa ccatattcca ctattaaata acttattgtg gctgaacttt 4561 caaaaacaat ttttcgaatt atatttgtgc ttatgttgta aggtatgctg ccaaatattt 4621 tatatggatt gtggctagga aatgtaaatt tcagtatatc atcatttact atttgatagt 4681 taggataatt taagagctta ttacgagtta cctcacataa tttagaatca atttctatcg 4741 ccgttacaaa attacatctc tttaccaatc cagcagtaaa atgacctttc cctgcaccta 4801 tttcaaagat gttatctttt tcatctaaac ttatgcaatt cattattttt tctatgtgat 4861 attttgaagt aataaaattt tgactatctt ttatatttac tttgttcatt ataacctctc 4921 cttaatttat tgcatctctt ttcgaatatt tatgtttttt gagaaaagaa cgtactcatg 4981 gttcatcccg atatgcgtat cggtctgtat atcagcaact ttctatgtgt ttcaactaca 5041 atagtcatct attctcatct ttctgagtcc accccctgca aagcccctct ttacgacata 5101 aaaattcggt cggaaaaggt atgcaaaaga tgtttctctc tttaagagaa actcttcggg 5161 atgcaaaaat atgaaaataa ctccaattca ccaaattata tagcgacttt tttacaaaat 5221 gctaaaattt gttgatttcc gtcaagcaat tgttgagcaa aaatgtcttt tacgataaaa 5281 tgatacctca atatcaactg tttagcaaaa cgatatttct cttaaagaga gaaacacctt 5341 tttgttcacc aatccccgac ttttaatccc gcggccatga ttgaaaaagg aagagtatga 5401 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 5461 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 5521 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 5581 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 5641 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 5701 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 5761 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 5821 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 5881 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 5941 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 6001 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 6061 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 6121 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 6181 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 6241 tgattaagca ttggtaactg tcagaccaag tttactcata acgcgtcaat tcgaggggga 6301 tcaattccgt gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct 6361 tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga 6421 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta 6481 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc 6541 tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa 6601 tgaccccgaa gcagggttat gcagcggaaa acggaattga tccggccacg atgcgtccgg 6661 cgtagaggat ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt 6721 ttcacgtact aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc 6781 tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact 6841 aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg 6901 ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac 6961 ggttgtggac aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc 7021 aattttgagt gacacaggaa cacttaacgg ctgacatggg aattcccctc caccgcggtg 7081 g //