LOCUS       pMM596                  7081 bp    DNA     circular UNA 08-JUL-2015
DEFINITION  pNBU2-LacI-PcfxA-LacO13-NanoLuc.
ACCESSION   urn.local...77-4vxo27y
VERSION     urn.local...77-4vxo27y
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..98
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="PcfxA"
     protein_bind    44..64
                     /created_by="User"
                     /label="LacO1"
     misc_feature    63..66
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="-33"
     misc_feature    87..94
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="-7"
     misc_feature    99
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="+1"
     protein_bind    100..120
                     /created_by="User"
                     /label="LacO1"
     gene            163..678
                     /gene="NanoLuc"
                     /Transferred_From="pD881-SR:145030 PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="NanoLuc"
     terminator      722..773
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="Terminator"
     promoter        834..1142
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="Pbt1311"
     CDS             1143..2234
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="lacIq"
     terminator      2241..2301
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="L3S2P21 Terminator"
     CDS             complement(2455..3735)
                     /note="NBU2 integrase"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="NBU2 integrase"
     misc_recomb     3895..3917
                     /note="attN2"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="attN2"
     CDS             complement(4175..4909)
                     /note="ermG"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="ermG"
     CDS             5397..6258
                     /note="beta-lactamase from pBR322"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="beta-lactamase from pBR322"
     misc_recomb     6296..7081
                     /note="RP4 oriT/RSK oriV"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="RP4 oriT/RSK oriV"
ORIGIN      
        1 ttacaaagaa aattcgacaa actgttattt ttctatctat ttaaattgtg agcggataac
       61 aatttgggtg ggaaacttta gttatgtacc tttgtcggca attgtgagcg gataacaatt
      121 aaataaagat attctcgtca aacaaatata aataatataa acatggtttt tactctggaa
      181 gattttgttg gcgattggcg tcagaccgcg ggttataatt tggatcaagt cctggaacag
      241 ggtggcgtaa gctctctgtt ccagaacctg ggtgtgagcg tgacgccgat tcagcgcatc
      301 gttctgtccg gcgagaacgg tctgaaaatt gatattcatg tgatcatccc gtacgaaggc
      361 ctgagcggtg accaaatggg tcaaatcgag aaaatcttta aagtcgtcta cccagttgac
      421 gatcaccact tcaaggttat cttgcattac ggtacgctgg tgattgatgg tgtgaccccg
      481 aatatgattg actatttcgg ccgtccgtat gaaggcattg ccgtttttga cggtaaaaag
      541 atcaccgtca ccggtaccct gtggaatggc aataagatta ttgacgagcg tctgattaac
      601 ccggacggca gcctgctgtt ccgcgtgacc atcaacggtg tcacgggttg gcgtctgtgc
      661 gagcgcatcc tggcataatg aactgcactt gctttgataa ttaatgataa acaatctaaa
      721 agcactctaa tcgttatcgg agtgctttta gattactaat caaattgctt ctactaattg
      781 cctatcttcc agtgatggaa cagcatttgt gcattggctg caacaatcag ccttgatctg
      841 gaagaagcaa tgaaagctgc tgttaagtct ccgaatcagg tattgttcct gacaggtgta
      901 ttcccatccg gtaaacgcgg atactttgca gttgatctga ctcaggaata aattataaat
      961 taaggtaaga agattgtagg ataagctaat gaaatagaaa aaggatgccg tcacacaact
     1021 tgtcggcatt cttttttgtt ttattagttg aaaatatagt gaaaaagttg cctaaatatg
     1081 tatgttaaca aattatttgt cgtaactttg cactccaaat ctgtttttaa catatggcac
     1141 tagtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     1201 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     1261 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     1321 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     1381 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     1441 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     1501 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     1561 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     1621 acagtattat tttctcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat
     1681 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     1741 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     1801 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     1861 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     1921 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     1981 aggacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     2041 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     2101 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     2161 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2221 aaagcgggca gtgagctttc ctcggtacca aattccagaa aagaggcctc ccgaaagggg
     2281 ggcctttttt cgttttggtc ctacttgtgc ctgttctatt tccgaaccga ccgcttgtat
     2341 gaatccatca aaattcgttt tctctatgtt ggattccttg ttgctcatat tgtgatgata
     2401 atttctacaa atatagtcat tggtaactat ctatgaaact gtttgatact tttatagttg
     2461 attaaacttg ttcatggcat ttgccttaat atcatccgct atgtcaatgt agggtttcat
     2521 agctttgtag tcgctgtgtc ccgtccattt catgaccacc tgtgccggga ttccgagagc
     2581 cagcgcattg cagatgaatg tccttcttcc tgcatgggta ctgagcaaag cgtatttggg
     2641 tgtgacttca tcaatacgtt catttccctt gtagtaggtt tcccgtacag gctcgttgat
     2701 ttctgccagt tcgcccagct ctttcaggta atcgttcatc ttctggttgc tgatgacggg
     2761 cagagccatg taattctcga aatggatgtc cttgtatttg tccagtatgg ctttgctgta
     2821 tttgttcagt tcaatcgtca ggctgtcggc agtcttgact gtggttattt cgatgtggtc
     2881 ggacttcaca tcgcttcttt tcagattgcg aacatccgaa taccgcaaac tcgtaaagca
     2941 gcagaacagg aaaacatcac gcacacgttc caggtattgc ttatccttgg gtatctggta
     3001 gtctttcagc ttgttcagtt catcccaagt caggaagatt acttttttcg aggtggtttt
     3061 cagtttcggt ttgaacgtat cgtatgcaat gttctgatga tgtcctttct tgaagctcca
     3121 gcgcaggaac catttgagga atcccatttg cttgccgatg gtgctgtttc tcatatcctt
     3181 ggtgtcacgc aggaagttga cgtattcgtt caatccaaac tcgttgaaat agttgaacgt
     3241 tgcatcctcc ttgaactctt tgaggtggtt cctcactgct gcaaattttt cataggtgga
     3301 tgccgtccag ttattctggt taccgcactc ttttacaaac tcatcgaaca cctcccaaaa
     3361 gctgacaggg gcttcttccg gctgttcttc gctggtgtct ttcattctca tgttgaaagc
     3421 ttccttcaac tgttgggtcg ttggcatgac ctcctgcacc tcaaattcct tgaaaatatt
     3481 ctggatttcg gcatagtatt tcagcaagtc cgtattgatt tcggctgcac tttgctttag
     3541 cttgttggta catccgctct ttacccgctg cttatctgca tcccatttgg ctacgtcaat
     3601 ccggtagccc gttgtaaact cgatgcgttg gctggcaaag atgacacgca tacggatggg
     3661 tacgttctct acgattggca caccgttctt tttccggctc tccaatgcaa aaatgatgtt
     3721 gcgcttgata ttcataattg ggtgcgtttg aaattctaca cccaaatata cacccaatta
     3781 ttgagatagc aaaagacatt tagaaacatt tacttttact ctatattgta atttacactt
     3841 gattatcagt cgtttgcagt cttatgatat tctgtgaaag tataagttcg agagcctgtc
     3901 tctccgcaaa aaacgctgaa aatcagcaga ttgcaaaaca aacaccctgt tttacaccca
     3961 agaatgtaaa gtcggctgtt tttgttttat ttaagataat acaaccacta cataataaaa
     4021 gagtagcgat attaaaagaa tccgatgaga aaagactaat atttatctat ccattcagtt
     4081 tgatttttca ggactttaca tcgtcctgaa agtatttgtt ggtaccggta ccgaggacgc
     4141 gtaaacattt acagttgcat gtggcctatt gtttttagcc gttaaatatt ttataactat
     4201 taaatagcga tacaaattgt tcgaaactaa tattgtttat atcatatatt ctcgcatgtt
     4261 ttaaagcttt attaaattga ttttttgtaa acagtttttc gtactctttg ttaacccatt
     4321 tcattacaaa agtttcatat ttttttctct ctttaaatgc catttttgct ggctttcttt
     4381 ttaatacaat taatgtgcta tccactttag gttttggatg gaaataatac ctaggaattt
     4441 ttgctaatat agaaatatct acctctgcca ttaacagcaa tgctagtgat ctgtttgtat
     4501 ctaataacat tttagcaaaa ccatattcca ctattaaata acttattgtg gctgaacttt
     4561 caaaaacaat ttttcgaatt atatttgtgc ttatgttgta aggtatgctg ccaaatattt
     4621 tatatggatt gtggctagga aatgtaaatt tcagtatatc atcatttact atttgatagt
     4681 taggataatt taagagctta ttacgagtta cctcacataa tttagaatca atttctatcg
     4741 ccgttacaaa attacatctc tttaccaatc cagcagtaaa atgacctttc cctgcaccta
     4801 tttcaaagat gttatctttt tcatctaaac ttatgcaatt cattattttt tctatgtgat
     4861 attttgaagt aataaaattt tgactatctt ttatatttac tttgttcatt ataacctctc
     4921 cttaatttat tgcatctctt ttcgaatatt tatgtttttt gagaaaagaa cgtactcatg
     4981 gttcatcccg atatgcgtat cggtctgtat atcagcaact ttctatgtgt ttcaactaca
     5041 atagtcatct attctcatct ttctgagtcc accccctgca aagcccctct ttacgacata
     5101 aaaattcggt cggaaaaggt atgcaaaaga tgtttctctc tttaagagaa actcttcggg
     5161 atgcaaaaat atgaaaataa ctccaattca ccaaattata tagcgacttt tttacaaaat
     5221 gctaaaattt gttgatttcc gtcaagcaat tgttgagcaa aaatgtcttt tacgataaaa
     5281 tgatacctca atatcaactg tttagcaaaa cgatatttct cttaaagaga gaaacacctt
     5341 tttgttcacc aatccccgac ttttaatccc gcggccatga ttgaaaaagg aagagtatga
     5401 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5461 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5521 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5581 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5641 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5701 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5761 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5821 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5881 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5941 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6001 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6061 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6121 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6181 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6241 tgattaagca ttggtaactg tcagaccaag tttactcata acgcgtcaat tcgaggggga
     6301 tcaattccgt gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct
     6361 tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga
     6421 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta
     6481 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc
     6541 tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa
     6601 tgaccccgaa gcagggttat gcagcggaaa acggaattga tccggccacg atgcgtccgg
     6661 cgtagaggat ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt
     6721 ttcacgtact aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc
     6781 tcatggctaa cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact
     6841 aagctctcat gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg
     6901 ttttaagttt tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac
     6961 ggttgtggac aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc
     7021 aattttgagt gacacaggaa cacttaacgg ctgacatggg aattcccctc caccgcggtg
     7081 g
//
