LOCUS       pMM710                 10860 bp    DNA     circular UNA 08-JUL-2015
DEFINITION  pNBU2-BtdCas9-NS.
ACCESSION   urn.local...f-4vxgq8t
VERSION     urn.local...f-4vxgq8t
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     CDS             <10746..>747
                     /note="beta-lactamase from pBR322"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="beta-lactamase from pBR322"
     misc_recomb     785..1570
                     /note="RP4 oriT/RSK oriV"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="RP4 oriT/RSK oriV"
     promoter        1571..1649
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="PcfxA"
     misc_feature    1614..1617
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="-33"
     protein_bind    1618..1637
                     /created_by="User"
                     /label="LacO1'"
     misc_feature    1638..1645
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="-7"
     misc_feature    1650
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="+1"
     protein_bind    1651..1671
                     /created_by="User"
                     /label="LacO1"
     misc_difference order(1676..1680,1676..1680)
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="RBS?"
     misc_difference order(1704..1706,1704..1706)
                     /created_by="User"
                     /X-Transferred_From="PcfxA-sfGFP PCR Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="RBS?"
     misc_feature    <1710..1713
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     gene            1714..5820
                     /created_by="User"
                     /Transferred_From="MBP-Cas9 Strep pyogenes PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="cas9"
     modified_base   1742
                     /created_by="User"
                     /label="D10A"
     misc_feature    4231..4232
                     /Original_Bases="CA"
                     /label="CA"
                     /note="Geneious type: Editing History Replacement"
     modified_base   4231..4232
                     /created_by="User"
                     /label="H840A"
     misc_feature    4233
                     /created_by="User"
                     /label
     misc_feature    <5817..5820
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     terminator      5836..5865
                     /created_by="User"
                     /modified_by="User"
                     /label="tL17"
     source          complement(<5881..>5915)
                     /organism="Bacteroides thetaiotaomicron VPI-5482"
                     /mol_type="genomic DNA"
                     /strain="VPI-5482"
                     /db_xref="taxon:226186"
                     /label="source Bacteroides thetaiotaomicron VPI-5482"
     promoter        5916..5959
                     /created_by="User"
                     /label="P1"
     misc_feature    5923..5926
                     /created_by="User"
                     /label="-33"
     misc_feature    5948..5955
                     /created_by="User"
                     /label="-7"
     origin          5950^5951
                     /label="origin"
     CRISPR          5960..5979
                     /created_by="User"
                     /label="Scramble2"
     misc_feature    5960..5985
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ncRNA           5980..6021
                     /created_by="User"
                     /Transferred_From="sgRNA GFP1"
                     /Transferred_Similarity="100.00%"
                     /label="Cas9 Adaptor"
     misc_feature    6015..10860
                     /label="pNBU2-Lac-dCas9-NL1 PCR Product"
                     /note="Geneious type: Inserted sequence"
     terminator      6022..6061
                     /created_by="User"
                     /Transferred_From="sgRNA GFP1"
                     /Transferred_Similarity="100.00%"
                     /label="SpyTerm"
     misc_feature    6122^6123
                     /Original_Bases="CCACGTGCTAAGGAGTGAGCTTCTCGGATTTTATTTGTATT
                     TTTGCCATGCCTGATGAGGTTTTGTTTGATTATTTTTTTGCAACACTAAGTTAAGTGA
                     ATCCTCTGACATGGCAAAATCCTGAGCAACTTTTTGTTGCTCAGGTACTTAAAAAAAA
                     TATTTTATAATAGTGTTGCGGAATTAAGGTAAAAGAAATGGTTTTTACTCTGGAAGAT
                     TTTGTTGGCGATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGG
                     GTGGCGTAAGCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCAT
                     CGTTCTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAA
                     GGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTACCCAG
                     TTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATTGATGGTGT
                     GACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATTGCCGTTTTTGAC
                     GGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAATAAGATTATTGACGAGC
                     GTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGTGACCATCAACGGTGTCACGGG
                     TTGGCGTCTGTGCGAGCGCATCCTGGCATAATGAACTGCACTTGCTTTGATAATTAAT
                     GATAAACAATCTAAAAGCACTCTAATCGTTATCGGAGTGCTTTTAGATTACTAATCAA
                     ATTGCTTCTA"
                     /label="CCACGTGCTAAGGAGTGAGCTTCTCGGATTTTATTTGTATTTTTGCCATG
                     CCTGATGAGGTTTTGTTTGATTATTTTTTTGCAACACTAAGTTAAGTGAATCCTCTGA
                     CATGGCAAAATCCTGAGCAACTTTTTGTTGCTCAGGTACTTAAAAAAAATATTTTATA
                     ATAGTGTTGCGGAATTAAGGTAAAAGAAATGGTTTTTACTCTGGAAGATTTTGTTGGC
                     GATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAA
                     GCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTTCTGTC
                     CGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAAGGCCTGAGC
                     GGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTACCCAGTTGACGATC
                     ACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATTGATGGTGTGACCCCGAA
                     TATGATTGACTATTTCGGCCGTCCGTATGAAGGCATTGCCGTTTTTGACGGTAAAAAG
                     ATCACCGTCACCGGTACCCTGTGGAATGGCAATAAGATTATTGACGAGCGTCTGATTA
                     ACCCGGACGGCAGCCTGCTGTTCCGCGTGACCATCAACGGTGTCACGGGTTGGCGTCT
                     GTGCGAGCGCATCCTGGCATAATGAACTGCACTTGCTTTGATAATTAATGATAAACAA
                     TCTAAAAGCACTCTAATCGTTATCGGAGTGCTTTTAGATTACTAATCAAATTGCTTCT
                     A"
                     /note="Geneious type: Editing History Deletion"
     promoter        6183..6491
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="Pbt1311"
     CDS             6492..7583
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="lacIq"
     terminator      7590..7650
                     /created_by="User"
                     /Transferred_From="LacIq Casette PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="L3S2P21 Terminator"
     misc_difference 7651..7688
                     /created_by="User"
                     /label
     CDS             complement(7804..9084)
                     /note="NBU2 integrase"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="NBU2 integrase"
     misc_recomb     9244..9266
                     /note="attN2"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="attN2"
     CDS             complement(9524..10258)
                     /note="ermG"
                     /X-Transferred_From="pNBU2_ermGb_usBT (modified) PCR
                     Product"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pNBU2-PcfxA-sfGFP PCR Product"
                     /Transferred_Similarity="100.00%"
                     /label="ermG"
ORIGIN      
        1 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
       61 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcataa cgcgtcaatt
      781 cgagggggat caattccgtg ataggtgggc tgcccttcct ggttggcttg gtttcatcag
      841 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
      901 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
      961 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
     1021 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
     1081 tcgctataat gaccccgaag cagggttatg cagcggaaaa cggaattgat ccggccacga
     1141 tgcgtccggc gtagaggatc tgaagatcag cagttcaacc tgttgatagt acgtactaag
     1201 ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga
     1261 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt
     1321 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag
     1381 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta
     1441 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc
     1501 tctcaaagca attttgagtg acacaggaac acttaacggc tgacatggga attcccctcc
     1561 accgcggtgg ttacaaagaa aattcgacaa actgttattt ttctatctat ttatttgaat
     1621 tgtgagcgga taacaattac ctttgtcggc aattgtgagc ggataacaat taaataaaga
     1681 tattctcgtc aaacaaatat aaataatata aacatggata agaaatactc aataggctta
     1741 gctatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct
     1801 aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg
     1861 gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt
     1921 agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag
     1981 atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa
     2041 gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat
     2101 gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg
     2161 gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg
     2221 attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta
     2281 caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa
     2341 gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc
     2401 cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc
     2461 cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat
     2521 acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg
     2581 tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat
     2641 actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat
     2701 caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa
     2761 atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa
     2821 gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta
     2881 ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct
     2941 attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt
     3001 tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct
     3061 tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct
     3121 gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa
     3181 tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca
     3241 aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat
     3301 gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt
     3361 gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc
     3421 aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct
     3481 tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat
     3541 gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg
     3601 gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa
     3661 cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt
     3721 attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc
     3781 aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa
     3841 aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt
     3901 agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa
     3961 gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca
     4021 actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa
     4081 gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa
     4141 aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat
     4201 attaatcgtt taagtgatta tgatgtcgat gccattgttc cacaaagttt ccttaaagac
     4261 gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac
     4321 gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc
     4381 aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt
     4441 gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag
     4501 catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt
     4561 attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat
     4621 ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta
     4681 aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc
     4741 tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata
     4801 ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa
     4861 attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact
     4921 ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg
     4981 ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca
     5041 attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa
     5101 aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg
     5161 gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg
     5221 gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa
     5281 gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt
     5341 cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca
     5401 agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca
     5461 gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt
     5521 attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa
     5581 gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt
     5641 attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca
     5701 acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat
     5761 caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactga
     5821 attaatgcgg ctgcaatttt tttgggcggg gccgcccaaa aaaatcctag caccctgcag
     5881 cagtactgct tgaccataag aacaaaaaaa cttccgataa agtttggaag ataaagctaa
     5941 aagttcttat ctttgcagtc tggaatgaat tggcctatgg ttttagagct agaaatagca
     6001 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     6061 ttctagatga tgattaagga tcttgcgtac aatttgacga tcgtttcacg ctaagatgct
     6121 ggctaattgc ctatcttcca gtgatggaac agcatttgtg cattggctgc aacaatcagc
     6181 cttgatctgg aagaagcaat gaaagctgct gttaagtctc cgaatcaggt attgttcctg
     6241 acaggtgtat tcccatccgg taaacgcgga tactttgcag ttgatctgac tcaggaataa
     6301 attataaatt aaggtaagaa gattgtagga taagctaatg aaatagaaaa aggatgccgt
     6361 cacacaactt gtcggcattc ttttttgttt tattagttga aaatatagtg aaaaagttgc
     6421 ctaaatatgt atgttaacaa attatttgtc gtaactttgc actccaaatc tgtttttaac
     6481 atatggcact agtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     6541 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     6601 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     6661 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     6721 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     6781 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     6841 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     6901 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     6961 cacccatcaa cagtattatt ttctcccatg aggacggtac gcgactgggc gtggagcatc
     7021 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     7081 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     7141 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     7201 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     7261 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     7321 acgataccga ggacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     7381 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     7441 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     7501 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7561 cccgactgga aagcgggcag tgagctttcc tcggtaccaa attccagaaa agaggcctcc
     7621 cgaaaggggg gccttttttc gttttggtcc tacttgtgcc tgttctattt ccgaaccgac
     7681 cgcttgtatg aatccatcaa aattcgtttt ctctatgttg gattccttgt tgctcatatt
     7741 gtgatgataa tttctacaaa tatagtcatt ggtaactatc tatgaaactg tttgatactt
     7801 ttatagttga ttaaacttgt tcatggcatt tgccttaata tcatccgcta tgtcaatgta
     7861 gggtttcata gctttgtagt cgctgtgtcc cgtccatttc atgaccacct gtgccgggat
     7921 tccgagagcc agcgcattgc agatgaatgt ccttcttcct gcatgggtac tgagcaaagc
     7981 gtatttgggt gtgacttcat caatacgttc atttcccttg tagtaggttt cccgtacagg
     8041 ctcgttgatt tctgccagtt cgcccagctc tttcaggtaa tcgttcatct tctggttgct
     8101 gatgacgggc agagccatgt aattctcgaa atggatgtcc ttgtatttgt ccagtatggc
     8161 tttgctgtat ttgttcagtt caatcgtcag gctgtcggca gtcttgactg tggttatttc
     8221 gatgtggtcg gacttcacat cgcttctttt cagattgcga acatccgaat accgcaaact
     8281 cgtaaagcag cagaacagga aaacatcacg cacacgttcc aggtattgct tatccttggg
     8341 tatctggtag tctttcagct tgttcagttc atcccaagtc aggaagatta cttttttcga
     8401 ggtggttttc agtttcggtt tgaacgtatc gtatgcaatg ttctgatgat gtcctttctt
     8461 gaagctccag cgcaggaacc atttgaggaa tcccatttgc ttgccgatgg tgctgtttct
     8521 catatccttg gtgtcacgca ggaagttgac gtattcgttc aatccaaact cgttgaaata
     8581 gttgaacgtt gcatcctcct tgaactcttt gaggtggttc ctcactgctg caaatttttc
     8641 ataggtggat gccgtccagt tattctggtt accgcactct tttacaaact catcgaacac
     8701 ctcccaaaag ctgacagggg cttcttccgg ctgttcttcg ctggtgtctt tcattctcat
     8761 gttgaaagct tccttcaact gttgggtcgt tggcatgacc tcctgcacct caaattcctt
     8821 gaaaatattc tggatttcgg catagtattt cagcaagtcc gtattgattt cggctgcact
     8881 ttgctttagc ttgttggtac atccgctctt tacccgctgc ttatctgcat cccatttggc
     8941 tacgtcaatc cggtagcccg ttgtaaactc gatgcgttgg ctggcaaaga tgacacgcat
     9001 acggatgggt acgttctcta cgattggcac accgttcttt ttccggctct ccaatgcaaa
     9061 aatgatgttg cgcttgatat tcataattgg gtgcgtttga aattctacac ccaaatatac
     9121 acccaattat tgagatagca aaagacattt agaaacattt acttttactc tatattgtaa
     9181 tttacacttg attatcagtc gtttgcagtc ttatgatatt ctgtgaaagt ataagttcga
     9241 gagcctgtct ctccgcaaaa aacgctgaaa atcagcagat tgcaaaacaa acaccctgtt
     9301 ttacacccaa gaatgtaaag tcggctgttt ttgttttatt taagataata caaccactac
     9361 ataataaaag agtagcgata ttaaaagaat ccgatgagaa aagactaata tttatctatc
     9421 cattcagttt gatttttcag gactttacat cgtcctgaaa gtatttgttg gtaccggtac
     9481 cgaggacgcg taaacattta cagttgcatg tggcctattg tttttagccg ttaaatattt
     9541 tataactatt aaatagcgat acaaattgtt cgaaactaat attgtttata tcatatattc
     9601 tcgcatgttt taaagcttta ttaaattgat tttttgtaaa cagtttttcg tactctttgt
     9661 taacccattt cattacaaaa gtttcatatt tttttctctc tttaaatgcc atttttgctg
     9721 gctttctttt taatacaatt aatgtgctat ccactttagg ttttggatgg aaataatacc
     9781 taggaatttt tgctaatata gaaatatcta cctctgccat taacagcaat gctagtgatc
     9841 tgtttgtatc taataacatt ttagcaaaac catattccac tattaaataa cttattgtgg
     9901 ctgaactttc aaaaacaatt tttcgaatta tatttgtgct tatgttgtaa ggtatgctgc
     9961 caaatatttt atatggattg tggctaggaa atgtaaattt cagtatatca tcatttacta
    10021 tttgatagtt aggataattt aagagcttat tacgagttac ctcacataat ttagaatcaa
    10081 tttctatcgc cgttacaaaa ttacatctct ttaccaatcc agcagtaaaa tgacctttcc
    10141 ctgcacctat ttcaaagatg ttatcttttt catctaaact tatgcaattc attatttttt
    10201 ctatgtgata ttttgaagta ataaaatttt gactatcttt tatatttact ttgttcatta
    10261 taacctctcc ttaatttatt gcatctcttt tcgaatattt atgttttttg agaaaagaac
    10321 gtactcatgg ttcatcccga tatgcgtatc ggtctgtata tcagcaactt tctatgtgtt
    10381 tcaactacaa tagtcatcta ttctcatctt tctgagtcca ccccctgcaa agcccctctt
    10441 tacgacataa aaattcggtc ggaaaaggta tgcaaaagat gtttctctct ttaagagaaa
    10501 ctcttcggga tgcaaaaata tgaaaataac tccaattcac caaattatat agcgactttt
    10561 ttacaaaatg ctaaaatttg ttgatttccg tcaagcaatt gttgagcaaa aatgtctttt
    10621 acgataaaat gatacctcaa tatcaactgt ttagcaaaac gatatttctc ttaaagagag
    10681 aaacaccttt ttgttcacca atccccgact tttaatcccg cggccatgat tgaaaaagga
    10741 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10801 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
//
