LOCUS Exported 5767 bp ds-DNA circular SYN 19-JUN-2015 DEFINITION synthetic circular DNA ACCESSION urn.local...1409162163307.292 VERSION . KEYWORDS pAT751 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5767) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 29, 2015 from SnapGene 2.7.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5767 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(113..1393) /label=NBU2 integrase /note="NBU2 integrase" misc_recomb 1553..1575 /label=attN2 /note="attN2" misc_recomb 1795..3939 /label=NluI fragment from pKNOCK /note="NluI fragment from pKNOCK" CDS complement(1833..2567) /label=ermG /note="ermG" CDS 3055..3916 /label=beta-lactamase from pBR322 /note="beta-lactamase from pBR322" misc_recomb 3954..4739 /label=RP4 oriT/RSK oriV /note="RP4 oriT/RSK oriV" misc_feature 4740..4746 /label=MCS [Split] /note="MCS [Split]" CDS 4741..4951 /label=confirmed by Sanger seq. /note="confirmed by Sanger seq" /note="confirmed by Sanger seq." misc_binding 4747..4951 /label=Sigma70 Promoter /note="Sigma70 Promoter" misc_feature 4952..4977 /note="fucO" misc_binding 4978..5055 /label=Sigma70 Promoter(1) /note="Sigma70 Promoter(1)" /note="Sigma70 Promoter" CDS 4978..5055 /label=confirmed by Sanger seq.(1) /note="confirmed by Sanger seq.(1)" /note="confirmed by Sanger seq." promoter 4981..4983 /label=-33 /note="-33 promoter" promoter 5013..5023 /label=-7 /note="-7 promoter" misc_feature complement(5023..5055) /label=BT1311 Homology /note="BT1311 Homology" /note="""Geneious type: primer_bind_reverse"" 5738..5767" gene 5056..5571 /gene="NanoLuc" /label=NanoLuc /note="NanoLuc" primer_bind 5056..5085 /label=mmD425 /note="mmD425" misc_feature complement(5550..5571) /label=NBU2 Homology /note="NBU2 Homology" /note="""Geneious type: primer_bind_reverse"" complement(517..554)" misc_feature complement(5550..5571) /label=BamHI-NanoLuc-CDS-R /note="BamHI-NanoLuc-CDS-R" primer_bind 5572..5605 /label=mmD418 /note="mmD418" terminator 5615..5666 /label=Terminator ORIGIN 1 atccatcaaa attcgttttc tctatgttgg attccttgtt gctcatattg tgatgataat 61 ttctacaaat atagtcattg gtaactatct atgaaactgt ttgatacttt tatagttgat 121 taaacttgtt catggcattt gccttaatat catccgctat gtcaatgtag ggtttcatag 181 ctttgtagtc gctgtgtccc gtccatttca tgaccacctg tgccgggatt ccgagagcca 241 gcgcattgca gatgaatgtc cttcttcctg catgggtact gagcaaagcg tatttgggtg 301 tgacttcatc aatacgttca tttcccttgt agtaggtttc ccgtacaggc tcgttgattt 361 ctgccagttc gcccagctct ttcaggtaat cgttcatctt ctggttgctg atgacgggca 421 gagccatgta attctcgaaa tggatgtcct tgtatttgtc cagtatggct ttgctgtatt 481 tgttcagttc aatcgtcagg ctgtcggcag tcttgactgt ggttatttcg atgtggtcgg 541 acttcacatc gcttcttttc agattgcgaa catccgaata ccgcaaactc gtaaagcagc 601 agaacaggaa aacatcacgc acacgttcca ggtattgctt atccttgggt atctggtagt 661 ctttcagctt gttcagttca tcccaagtca ggaagattac ttttttcgag gtggttttca 721 gtttcggttt gaacgtatcg tatgcaatgt tctgatgatg tcctttcttg aagctccagc 781 gcaggaacca tttgaggaat cccatttgct tgccgatggt gctgtttctc atatccttgg 841 tgtcacgcag gaagttgacg tattcgttca atccaaactc gttgaaatag ttgaacgttg 901 catcctcctt gaactctttg aggtggttcc tcactgctgc aaatttttca taggtggatg 961 ccgtccagtt attctggtta ccgcactctt ttacaaactc atcgaacacc tcccaaaagc 1021 tgacaggggc ttcttccggc tgttcttcgc tggtgtcttt cattctcatg ttgaaagctt 1081 ccttcaactg ttgggtcgtt ggcatgacct cctgcacctc aaattccttg aaaatattct 1141 ggatttcggc atagtatttc agcaagtccg tattgatttc ggctgcactt tgctttagct 1201 tgttggtaca tccgctcttt acccgctgct tatctgcatc ccatttggct acgtcaatcc 1261 ggtagcccgt tgtaaactcg atgcgttggc tggcaaagat gacacgcata cggatgggta 1321 cgttctctac gattggcaca ccgttctttt tccggctctc caatgcaaaa atgatgttgc 1381 gcttgatatt cataattggg tgcgtttgaa attctacacc caaatataca cccaattatt 1441 gagatagcaa aagacattta gaaacattta cttttactct atattgtaat ttacacttga 1501 ttatcagtcg tttgcagtct tatgatattc tgtgaaagta taagttcgag agcctgtctc 1561 tccgcaaaaa acgctgaaaa tcagcagatt gcaaaacaaa caccctgttt tacacccaag 1621 aatgtaaagt cggctgtttt tgttttattt aagataatac aaccactaca taataaaaga 1681 gtagcgatat taaaagaatc cgatgagaaa agactaatat ttatctatcc attcagtttg 1741 atttttcagg actttacatc gtcctgaaag tatttgttgg taccggtacc gaggacgcgt 1801 aaacatttac agttgcatgt ggcctattgt ttttagccgt taaatatttt ataactatta 1861 aatagcgata caaattgttc gaaactaata ttgtttatat catatattct cgcatgtttt 1921 aaagctttat taaattgatt ttttgtaaac agtttttcgt actctttgtt aacccatttc 1981 attacaaaag tttcatattt ttttctctct ttaaatgcca tttttgctgg ctttcttttt 2041 aatacaatta atgtgctatc cactttaggt tttggatgga aataatacct aggaattttt 2101 gctaatatag aaatatctac ctctgccatt aacagcaatg ctagtgatct gtttgtatct 2161 aataacattt tagcaaaacc atattccact attaaataac ttattgtggc tgaactttca 2221 aaaacaattt ttcgaattat atttgtgctt atgttgtaag gtatgctgcc aaatatttta 2281 tatggattgt ggctaggaaa tgtaaatttc agtatatcat catttactat ttgatagtta 2341 ggataattta agagcttatt acgagttacc tcacataatt tagaatcaat ttctatcgcc 2401 gttacaaaat tacatctctt taccaatcca gcagtaaaat gacctttccc tgcacctatt 2461 tcaaagatgt tatctttttc atctaaactt atgcaattca ttattttttc tatgtgatat 2521 tttgaagtaa taaaattttg actatctttt atatttactt tgttcattat aacctctcct 2581 taatttattg catctctttt cgaatattta tgttttttga gaaaagaacg tactcatggt 2641 tcatcccgat atgcgtatcg gtctgtatat cagcaacttt ctatgtgttt caactacaat 2701 agtcatctat tctcatcttt ctgagtccac cccctgcaaa gcccctcttt acgacataaa 2761 aattcggtcg gaaaaggtat gcaaaagatg tttctctctt taagagaaac tcttcgggat 2821 gcaaaaatat gaaaataact ccaattcacc aaattatata gcgacttttt tacaaaatgc 2881 taaaatttgt tgatttccgt caagcaattg ttgagcaaaa atgtctttta cgataaaatg 2941 atacctcaat atcaactgtt tagcaaaacg atatttctct taaagagaga aacacctttt 3001 tgttcaccaa tccccgactt ttaatcccgc ggccatgatt gaaaaaggaa gagtatgagt 3061 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 3121 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 3181 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 3241 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 3301 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 3361 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 3421 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 3481 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 3541 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 3601 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 3661 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 3721 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 3781 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 3841 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 3901 attaagcatt ggtaactgtc agaccaagtt tactcataac gcgtcaattc gagggggatc 3961 aattccgtga taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg 4021 ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc 4081 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact 4141 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt 4201 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg 4261 accccgaagc agggttatgc agcggaaaac ggaattgatc cggccacgat gcgtccggcg 4321 tagaggatct gaagatcagc agttcaacct gttgatagta cgtactaagc tctcatgttt 4381 cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc 4441 atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa 4501 gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt 4561 ttaagtttta taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg 4621 ttgtggacaa caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa 4681 ttttgagtga cacaggaaca cttaacggct gacatgggaa ttcccctcca ccgcggtggc 4741 ggccgctgat ctggaagaag caatgaaagc tgctgttaag tctccgaatc aggtattgtt 4801 cctgacaggt gtattcccat ccggtaaacg cggatacttt gcagttgatc tgactcagga 4861 ataaattata aattaaggta agaagattgt aggataagct aatgaaatag aaaaaggatg 4921 ccgtcacaca acttgtcggc attctttttt gctttgcaac agcatagctc agcacagaag 4981 ttgcctaaat atgtatgtta acaaattatt tgtcgtaact ttgcactcca aatctgtttt 5041 taacatatgg cactaatggt ttttactctg gaagattttg ttggcgattg gcgtcagacc 5101 gcgggttata atttggatca agtcctggaa cagggtggcg taagctctct gttccagaac 5161 ctgggtgtga gcgtgacgcc gattcagcgc atcgttctgt ccggcgagaa cggtctgaaa 5221 attgatattc atgtgatcat cccgtacgaa ggcctgagcg gtgaccaaat gggtcaaatc 5281 gagaaaatct ttaaagtcgt ctacccagtt gacgatcacc acttcaaggt tatcttgcat 5341 tacggtacgc tggtgattga tggtgtgacc ccgaatatga ttgactattt cggccgtccg 5401 tatgaaggca ttgccgtttt tgacggtaaa aagatcaccg tcaccggtac cctgtggaat 5461 ggcaataaga ttattgacga gcgtctgatt aacccggacg gcagcctgct gttccgcgtg 5521 accatcaacg gtgtcacggg ttggcgtctg tgcgagcgca tcctggcata atgaactgca 5581 cttgctttga taattaatga taaacaatct aaaagcactc taatcgttat cggagtgctt 5641 ttagattact aatcaaattg cttctactaa ttgcctatct tccagtgatg gaacagcatt 5701 tgtgcattgg ctgcaacaat cagccttact tgtgcctgtt ctatttccga accgaccgct 5761 tgtatga //