LOCUS       Exported                5761 bp ds-DNA     circular SYN 29-JUN-2015
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1409162163307.292
VERSION     .
KEYWORDS    pAT587
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2015 from SnapGene 2.7.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(95..1375)
                     /label=NBU2 integrase
                     /note="NBU2 integrase"
     misc_recomb     1535..1557
                     /label=attN2
                     /note="attN2"
     misc_recomb     1777..3921
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(1815..2549)
                     /label=ermG
                     /note="ermG"
     CDS             3037..3898
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     misc_recomb     3936..4721
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     misc_feature    4722..4728
                     /label=MCS [Split]
                     /note="MCS [Split]"
     CDS             4723..5011
                     /label=confirmed by Sanger seq.
                     /note="confirmed by Sanger seq"
                     /note="confirmed by Sanger seq."
     misc_binding    4729..5011
                     /label=Sigma70 Promoter
                     /note="Sigma70 Promoter"
     promoter        4963..4965
                     /label=-33
                     /note="-33 promoter"
     promoter        4995..5005
                     /label=-7
                     /note="-7 promoter"
     misc_feature    complement(5005..5011)
                     /label=BT1311 Homology
                     /note="BT1311 Homology"
                     /note="""Geneious type: primer_bind_reverse"" 5738..5767"
     misc_feature    5012..5031
                     /label=GH022
                     /note="GH022"
     gene            5032..5547
                     /gene="NanoLuc"
                     /label=NanoLuc
                     /note="NanoLuc"
     primer_bind     5032..5061
                     /label=mmD425
                     /note="mmD425"
     misc_feature    complement(5526..5547)
                     /label=BamHI-NanoLuc-CDS-R
                     /note="BamHI-NanoLuc-CDS-R"
     misc_feature    complement(5526..5547)
                     /label=NBU2 Homology
                     /note="NBU2 Homology"
                     /note="""Geneious type: primer_bind_reverse"" 
                     complement(517..554)"
     primer_bind     5548..5581
                     /label=mmD418
                     /note="mmD418"
     terminator      5591..5642
                     /label=Terminator
ORIGIN
        1 tctctatgtt ggattccttg ttgctcatat tgtgatgata atttctacaa atatagtcat
       61 tggtaactat ctatgaaact gtttgatact tttatagttg attaaacttg ttcatggcat
      121 ttgccttaat atcatccgct atgtcaatgt agggtttcat agctttgtag tcgctgtgtc
      181 ccgtccattt catgaccacc tgtgccggga ttccgagagc cagcgcattg cagatgaatg
      241 tccttcttcc tgcatgggta ctgagcaaag cgtatttggg tgtgacttca tcaatacgtt
      301 catttccctt gtagtaggtt tcccgtacag gctcgttgat ttctgccagt tcgcccagct
      361 ctttcaggta atcgttcatc ttctggttgc tgatgacggg cagagccatg taattctcga
      421 aatggatgtc cttgtatttg tccagtatgg ctttgctgta tttgttcagt tcaatcgtca
      481 ggctgtcggc agtcttgact gtggttattt cgatgtggtc ggacttcaca tcgcttcttt
      541 tcagattgcg aacatccgaa taccgcaaac tcgtaaagca gcagaacagg aaaacatcac
      601 gcacacgttc caggtattgc ttatccttgg gtatctggta gtctttcagc ttgttcagtt
      661 catcccaagt caggaagatt acttttttcg aggtggtttt cagtttcggt ttgaacgtat
      721 cgtatgcaat gttctgatga tgtcctttct tgaagctcca gcgcaggaac catttgagga
      781 atcccatttg cttgccgatg gtgctgtttc tcatatcctt ggtgtcacgc aggaagttga
      841 cgtattcgtt caatccaaac tcgttgaaat agttgaacgt tgcatcctcc ttgaactctt
      901 tgaggtggtt cctcactgct gcaaattttt cataggtgga tgccgtccag ttattctggt
      961 taccgcactc ttttacaaac tcatcgaaca cctcccaaaa gctgacaggg gcttcttccg
     1021 gctgttcttc gctggtgtct ttcattctca tgttgaaagc ttccttcaac tgttgggtcg
     1081 ttggcatgac ctcctgcacc tcaaattcct tgaaaatatt ctggatttcg gcatagtatt
     1141 tcagcaagtc cgtattgatt tcggctgcac tttgctttag cttgttggta catccgctct
     1201 ttacccgctg cttatctgca tcccatttgg ctacgtcaat ccggtagccc gttgtaaact
     1261 cgatgcgttg gctggcaaag atgacacgca tacggatggg tacgttctct acgattggca
     1321 caccgttctt tttccggctc tccaatgcaa aaatgatgtt gcgcttgata ttcataattg
     1381 ggtgcgtttg aaattctaca cccaaatata cacccaatta ttgagatagc aaaagacatt
     1441 tagaaacatt tacttttact ctatattgta atttacactt gattatcagt cgtttgcagt
     1501 cttatgatat tctgtgaaag tataagttcg agagcctgtc tctccgcaaa aaacgctgaa
     1561 aatcagcaga ttgcaaaaca aacaccctgt tttacaccca agaatgtaaa gtcggctgtt
     1621 tttgttttat ttaagataat acaaccacta cataataaaa gagtagcgat attaaaagaa
     1681 tccgatgaga aaagactaat atttatctat ccattcagtt tgatttttca ggactttaca
     1741 tcgtcctgaa agtatttgtt ggtaccggta ccgaggacgc gtaaacattt acagttgcat
     1801 gtggcctatt gtttttagcc gttaaatatt ttataactat taaatagcga tacaaattgt
     1861 tcgaaactaa tattgtttat atcatatatt ctcgcatgtt ttaaagcttt attaaattga
     1921 ttttttgtaa acagtttttc gtactctttg ttaacccatt tcattacaaa agtttcatat
     1981 ttttttctct ctttaaatgc catttttgct ggctttcttt ttaatacaat taatgtgcta
     2041 tccactttag gttttggatg gaaataatac ctaggaattt ttgctaatat agaaatatct
     2101 acctctgcca ttaacagcaa tgctagtgat ctgtttgtat ctaataacat tttagcaaaa
     2161 ccatattcca ctattaaata acttattgtg gctgaacttt caaaaacaat ttttcgaatt
     2221 atatttgtgc ttatgttgta aggtatgctg ccaaatattt tatatggatt gtggctagga
     2281 aatgtaaatt tcagtatatc atcatttact atttgatagt taggataatt taagagctta
     2341 ttacgagtta cctcacataa tttagaatca atttctatcg ccgttacaaa attacatctc
     2401 tttaccaatc cagcagtaaa atgacctttc cctgcaccta tttcaaagat gttatctttt
     2461 tcatctaaac ttatgcaatt cattattttt tctatgtgat attttgaagt aataaaattt
     2521 tgactatctt ttatatttac tttgttcatt ataacctctc cttaatttat tgcatctctt
     2581 ttcgaatatt tatgtttttt gagaaaagaa cgtactcatg gttcatcccg atatgcgtat
     2641 cggtctgtat atcagcaact ttctatgtgt ttcaactaca atagtcatct attctcatct
     2701 ttctgagtcc accccctgca aagcccctct ttacgacata aaaattcggt cggaaaaggt
     2761 atgcaaaaga tgtttctctc tttaagagaa actcttcggg atgcaaaaat atgaaaataa
     2821 ctccaattca ccaaattata tagcgacttt tttacaaaat gctaaaattt gttgatttcc
     2881 gtcaagcaat tgttgagcaa aaatgtcttt tacgataaaa tgatacctca atatcaactg
     2941 tttagcaaaa cgatatttct cttaaagaga gaaacacctt tttgttcacc aatccccgac
     3001 ttttaatccc gcggccatga ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3061 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3121 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3181 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3241 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3301 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3361 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3421 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3481 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3541 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3601 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3661 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3721 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3781 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3841 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3901 tcagaccaag tttactcata acgcgtcaat tcgaggggga tcaattccgt gataggtggg
     3961 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
     4021 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
     4081 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
     4141 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
     4201 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
     4261 gcagcggaaa acggaattga tccggccacg atgcgtccgg cgtagaggat ctgaagatca
     4321 gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat
     4381 gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc
     4441 tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa
     4501 taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa
     4561 aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag
     4621 ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt gacacaggaa
     4681 cacttaacgg ctgacatggg aattcccctc caccgcggtg gcggccgctg atctggaaga
     4741 agcaatgaaa gctgctgtta agtctccgaa tcaggtattg ttcctgacag gtgtattccc
     4801 atccggtaaa cgcggatact ttgcagttga tctgactcag gaataaatta taaattaagg
     4861 taagaagatt gtaggataag ctaatgaaat agaaaaagga tgccgtcaca caacttgtcg
     4921 gcattctttt ttgttttatt agttgaaaat atagtgaaaa agttgcctaa atatgtatgt
     4981 taacaaatta tttgtcgtaa ctttgcactc ccatataaaa gaaaagacac catggttttt
     5041 actctggaag attttgttgg cgattggcgt cagaccgcgg gttataattt ggatcaagtc
     5101 ctggaacagg gtggcgtaag ctctctgttc cagaacctgg gtgtgagcgt gacgccgatt
     5161 cagcgcatcg ttctgtccgg cgagaacggt ctgaaaattg atattcatgt gatcatcccg
     5221 tacgaaggcc tgagcggtga ccaaatgggt caaatcgaga aaatctttaa agtcgtctac
     5281 ccagttgacg atcaccactt caaggttatc ttgcattacg gtacgctggt gattgatggt
     5341 gtgaccccga atatgattga ctatttcggc cgtccgtatg aaggcattgc cgtttttgac
     5401 ggtaaaaaga tcaccgtcac cggtaccctg tggaatggca ataagattat tgacgagcgt
     5461 ctgattaacc cggacggcag cctgctgttc cgcgtgacca tcaacggtgt cacgggttgg
     5521 cgtctgtgcg agcgcatcct ggcataatga actgcacttg ctttgataat taatgataaa
     5581 caatctaaaa gcactctaat cgttatcgga gtgcttttag attactaatc aaattgcttc
     5641 tactaattgc ctatcttcca gtgatggaac agcatttgtg cattggctgc aacaatcagc
     5701 cttacttgtg cctgttctat ttccgaaccg accgcttgta tgaatccatc aaaattcgtt
     5761 t
//
