LOCUS       Exported                6680 bp ds-DNA     circular SYN 19-JUN-2015
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1409162163307.292
VERSION     .
KEYWORDS    pAT891
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2015 from SnapGene 2.7.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(113..1393)
                     /label=NBU2 integrase
                     /note="NBU2 integrase"
     misc_recomb     1553..1575
                     /label=attN2
                     /note="attN2"
     misc_recomb     1795..3939
                     /label=NluI fragment from pKNOCK
                     /note="NluI fragment from pKNOCK"
     CDS             complement(1833..2567)
                     /label=ermG
                     /note="ermG"
     CDS             3055..3916
                     /label=beta-lactamase from pBR322
                     /note="beta-lactamase from pBR322"
     misc_recomb     3954..4739
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     misc_feature    4740..4746
                     /label=MCS [Split]
                     /note="MCS [Split]"
     CDS             4741..5029
                     /label=confirmed by Sanger seq.
                     /note="confirmed by Sanger seq"
                     /note="confirmed by Sanger seq."
     misc_binding    4747..5029
                     /label=Sigma70 Promoter
                     /note="Sigma70 Promoter"
     promoter        4981..4983
                     /label=-33
                     /note="-33 promoter"
     promoter        5013..5023
                     /label=-7
                     /note="-7 promoter"
     misc_feature    complement(5023..5029)
                     /label=BT1311 Homology
                     /note="BT1311 Homology"
                     /note="""Geneious type: primer_bind_reverse"" 5738..5767"
     misc_feature    5030..5055
                     /note="fucO"
     misc_feature    5056..5098
                     /label=rpiL* RBS
                     /note="rpiL* RBS"
     misc_feature    5099..6484
                     /label=New Feature
                     /note="Int8"
     primer_bind     6485..6518
                     /label=mmD418
                     /note="mmD418"
     terminator      6528..6579
                     /label=Terminator
ORIGIN
        1 atccatcaaa attcgttttc tctatgttgg attccttgtt gctcatattg tgatgataat
       61 ttctacaaat atagtcattg gtaactatct atgaaactgt ttgatacttt tatagttgat
      121 taaacttgtt catggcattt gccttaatat catccgctat gtcaatgtag ggtttcatag
      181 ctttgtagtc gctgtgtccc gtccatttca tgaccacctg tgccgggatt ccgagagcca
      241 gcgcattgca gatgaatgtc cttcttcctg catgggtact gagcaaagcg tatttgggtg
      301 tgacttcatc aatacgttca tttcccttgt agtaggtttc ccgtacaggc tcgttgattt
      361 ctgccagttc gcccagctct ttcaggtaat cgttcatctt ctggttgctg atgacgggca
      421 gagccatgta attctcgaaa tggatgtcct tgtatttgtc cagtatggct ttgctgtatt
      481 tgttcagttc aatcgtcagg ctgtcggcag tcttgactgt ggttatttcg atgtggtcgg
      541 acttcacatc gcttcttttc agattgcgaa catccgaata ccgcaaactc gtaaagcagc
      601 agaacaggaa aacatcacgc acacgttcca ggtattgctt atccttgggt atctggtagt
      661 ctttcagctt gttcagttca tcccaagtca ggaagattac ttttttcgag gtggttttca
      721 gtttcggttt gaacgtatcg tatgcaatgt tctgatgatg tcctttcttg aagctccagc
      781 gcaggaacca tttgaggaat cccatttgct tgccgatggt gctgtttctc atatccttgg
      841 tgtcacgcag gaagttgacg tattcgttca atccaaactc gttgaaatag ttgaacgttg
      901 catcctcctt gaactctttg aggtggttcc tcactgctgc aaatttttca taggtggatg
      961 ccgtccagtt attctggtta ccgcactctt ttacaaactc atcgaacacc tcccaaaagc
     1021 tgacaggggc ttcttccggc tgttcttcgc tggtgtcttt cattctcatg ttgaaagctt
     1081 ccttcaactg ttgggtcgtt ggcatgacct cctgcacctc aaattccttg aaaatattct
     1141 ggatttcggc atagtatttc agcaagtccg tattgatttc ggctgcactt tgctttagct
     1201 tgttggtaca tccgctcttt acccgctgct tatctgcatc ccatttggct acgtcaatcc
     1261 ggtagcccgt tgtaaactcg atgcgttggc tggcaaagat gacacgcata cggatgggta
     1321 cgttctctac gattggcaca ccgttctttt tccggctctc caatgcaaaa atgatgttgc
     1381 gcttgatatt cataattggg tgcgtttgaa attctacacc caaatataca cccaattatt
     1441 gagatagcaa aagacattta gaaacattta cttttactct atattgtaat ttacacttga
     1501 ttatcagtcg tttgcagtct tatgatattc tgtgaaagta taagttcgag agcctgtctc
     1561 tccgcaaaaa acgctgaaaa tcagcagatt gcaaaacaaa caccctgttt tacacccaag
     1621 aatgtaaagt cggctgtttt tgttttattt aagataatac aaccactaca taataaaaga
     1681 gtagcgatat taaaagaatc cgatgagaaa agactaatat ttatctatcc attcagtttg
     1741 atttttcagg actttacatc gtcctgaaag tatttgttgg taccggtacc gaggacgcgt
     1801 aaacatttac agttgcatgt ggcctattgt ttttagccgt taaatatttt ataactatta
     1861 aatagcgata caaattgttc gaaactaata ttgtttatat catatattct cgcatgtttt
     1921 aaagctttat taaattgatt ttttgtaaac agtttttcgt actctttgtt aacccatttc
     1981 attacaaaag tttcatattt ttttctctct ttaaatgcca tttttgctgg ctttcttttt
     2041 aatacaatta atgtgctatc cactttaggt tttggatgga aataatacct aggaattttt
     2101 gctaatatag aaatatctac ctctgccatt aacagcaatg ctagtgatct gtttgtatct
     2161 aataacattt tagcaaaacc atattccact attaaataac ttattgtggc tgaactttca
     2221 aaaacaattt ttcgaattat atttgtgctt atgttgtaag gtatgctgcc aaatatttta
     2281 tatggattgt ggctaggaaa tgtaaatttc agtatatcat catttactat ttgatagtta
     2341 ggataattta agagcttatt acgagttacc tcacataatt tagaatcaat ttctatcgcc
     2401 gttacaaaat tacatctctt taccaatcca gcagtaaaat gacctttccc tgcacctatt
     2461 tcaaagatgt tatctttttc atctaaactt atgcaattca ttattttttc tatgtgatat
     2521 tttgaagtaa taaaattttg actatctttt atatttactt tgttcattat aacctctcct
     2581 taatttattg catctctttt cgaatattta tgttttttga gaaaagaacg tactcatggt
     2641 tcatcccgat atgcgtatcg gtctgtatat cagcaacttt ctatgtgttt caactacaat
     2701 agtcatctat tctcatcttt ctgagtccac cccctgcaaa gcccctcttt acgacataaa
     2761 aattcggtcg gaaaaggtat gcaaaagatg tttctctctt taagagaaac tcttcgggat
     2821 gcaaaaatat gaaaataact ccaattcacc aaattatata gcgacttttt tacaaaatgc
     2881 taaaatttgt tgatttccgt caagcaattg ttgagcaaaa atgtctttta cgataaaatg
     2941 atacctcaat atcaactgtt tagcaaaacg atatttctct taaagagaga aacacctttt
     3001 tgttcaccaa tccccgactt ttaatcccgc ggccatgatt gaaaaaggaa gagtatgagt
     3061 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3121 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3181 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3241 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3301 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3361 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3421 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3481 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3541 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3601 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3661 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3721 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3781 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3841 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3901 attaagcatt ggtaactgtc agaccaagtt tactcataac gcgtcaattc gagggggatc
     3961 aattccgtga taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg
     4021 ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc
     4081 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact
     4141 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
     4201 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg
     4261 accccgaagc agggttatgc agcggaaaac ggaattgatc cggccacgat gcgtccggcg
     4321 tagaggatct gaagatcagc agttcaacct gttgatagta cgtactaagc tctcatgttt
     4381 cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc
     4441 atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa
     4501 gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt
     4561 ttaagtttta taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg
     4621 ttgtggacaa caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa
     4681 ttttgagtga cacaggaaca cttaacggct gacatgggaa ttcccctcca ccgcggtggc
     4741 ggccgctgat ctggaagaag caatgaaagc tgctgttaag tctccgaatc aggtattgtt
     4801 cctgacaggt gtattcccat ccggtaaacg cggatacttt gcagttgatc tgactcagga
     4861 ataaattata aattaaggta agaagattgt aggataagct aatgaaatag aaaaaggatg
     4921 ccgtcacaca acttgtcggc attctttttt gttttattag ttgaaaatat agtgaaaaag
     4981 ttgcctaaat atgtatgtta acaaattatt tgtcgtaact ttgcactccc tttgcaacag
     5041 catagctcag cacagcgcat tttaaaataa aataaattat ttatttaatt aaacgaatat
     5101 gaaagttgcc gtttattgtc gtgttagcac cctggaacag aaagaacatg gtcatagcat
     5161 tgaagaacaa gagcgtaaac tgaaaagctt ctgcgatatt aatgattgga ccgtgtatga
     5221 tacctatatc gatgcaggtt atagcggtgc aaaacgtgat cgtccggaac tgcagcgtct
     5281 gatgaatgat attaacaaat ttgatctggt gctggtgtat aaactggatc gtctgacccg
     5341 taatgttcgt gatctgctgg acctgctgga aatctttgaa aaaaatgatg tgagctttcg
     5401 tagcgccacc gaagtttatg ataccaccac cgcaatgggt cgtctgtttg ttaccctggt
     5461 tggtgcaatg gcagaatggg aacgtgaaac cattcgtgaa cgtacccaga tgggtaaact
     5521 ggcagcactg cgtaaaggta ttatgctgac cacccctccg ttttattatg accgtgtgga
     5581 taataagttt gtgccgaaca aatacaaaga cgttattctg tgggcatatg acgaagcaat
     5641 gaaaggtcag agcgcaaaag caattgcacg caaactgaat aatagcgata ttccgcctcc
     5701 gaataatacc cagtggcagg gtcgtaccat tacccatgcc ctgcgtaatc cgtttacccg
     5761 tggtcatttt gattggggtg gtgtgcatat tgaaaataac catgaaccga tcatcaccga
     5821 tgagatgtat gagaaagtta aagatcgcct gaatgaacgc gtgaacacca aaaaagttcg
     5881 tcataccagc atttttcgtg gcaaactggt ttgtccggtt tgtaatgcac gcctgaccct
     5941 gaatagccat aaaaagaaaa gcaatagcgg ctatatcttt gtgaaacagt actactgcaa
     6001 caactgtaaa gttaccccga atctgaaacc ggtgtacatc aaagaaaaag aagtgattaa
     6061 agttttttac aattatctga aacgcttcga tctggaaaaa tatgaggtta cccagaaaca
     6121 gaacgaaccg gaaatcacca tcgatatcaa taaagttatg gaacagcgca aacgctacca
     6181 taaactgtat gcaagcggtc tgatgcaaga agatgaactg tttgacctga ttaaagaaac
     6241 cgatcagacc attgccgaat atgaaaaaca gaatgaaaac cgcgaagtga agcagtatga
     6301 tatcgaagat atcaaacagt ataaagatct gctgttagaa atgtgggata tcagctccga
     6361 tgaagataaa gaggacttta tcaaaatggc gattaaaaac atctattttg aatatatcat
     6421 tggcaccggt aacaccagcc gtaaacgtaa tagcctgaaa attacgagca ttgaattcta
     6481 ttaatgaact gcacttgctt tgataattaa tgataaacaa tctaaaagca ctctaatcgt
     6541 tatcggagtg cttttagatt actaatcaaa ttgcttctac taattgccta tcttccagtg
     6601 atggaacagc atttgtgcat tggctgcaac aatcagcctt acttgtgcct gttctatttc
     6661 cgaaccgacc gcttgtatga
//
