LOCUS       pTet-qacR_wt/pQa        3956 bp    DNA     circular UNA 17-AUG-2015
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...17-51owllq
VERSION     urn.local...17-51owllq
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    121..777
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /label="CmR"
     misc_feature    1190..2030
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /label="Ori-p15A (Chris)"
     promoter        2196..>2214
                     /created_by="User"
                     /label="tetR responsive promoter"
     promoter        <2215..2298
                     /created_by="User"
                     /label="tetR responsive promoter"
     5'UTR           2303..2320
                     /created_by="User"
                     /label="RBS"
     CDS             2322..2918
                     /created_by="User"
                     /modified_by="User"
                     /label="qacR wt"
     terminator      2920..3048
                     /created_by="User"
                     /label="Terminator"
     origin          3048^3049
                     /label="origin"
     terminator      complement(3081..3110)
                     /note="T500"
                     /label="T500 terminator"
     gene            order(complement(3139..3810),complement(3136..3138))
                     /created_by="User"
                     /modified_by="User"
                     /label="deGFP"
     misc_feature    complement(3816..3848)
                     /note="UTR1"
                     /label="UTR1"
     RBS             complement(3860..3867)
                     /created_by="User"
                     /label="RBS"
     promoter        3865..3916
                     /created_by="User"
                     /label="qacR promoter"
     promoter        3865..3869
                     /created_by="User"
                     /label="-44"
     protein_bind    3870..3897
                     /created_by="User"
                     /label="qacR binding site"
     promoter        3874..3879
                     /created_by="User"
                     /label="-35"
     promoter        complement(3884..3938)
                     /created_by="User"
                     /label="qacA promoter"
     promoter        complement(3899..3904)
                     /created_by="User"
                     /label="-10"
     promoter        3901..3906
                     /created_by="User"
                     /label="-10"
     promoter        complement(3922..3927)
                     /created_by="User"
                     /label="-35"
     promoter        complement(3934..3938)
                     /created_by="User"
                     /modified_by="User"
                     /label="-44"
ORIGIN      
        1 tatgaagctg agaccttgat cgggcacgta agaggttcca actttcacca taatgaaata
       61 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
      121 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
      181 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      241 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      301 cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt
      361 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
      421 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
      481 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
      541 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
      601 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
      661 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
      721 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
      781 tttgatatcg agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
      841 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
      901 cctcggtgag aatccaagcc tcgatcaacg tctcattttc gccaaaagtt ggcccagggc
      961 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     1021 ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg
     1081 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     1141 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcggt agcggagtgt
     1201 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     1261 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     1321 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     1381 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     1441 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     1501 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     1561 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     1621 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     1681 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     1741 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     1801 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     1861 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     1921 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
     1981 agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca
     2041 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa
     2101 ttctcatgtt tgacagctta tcatcgataa gcttccgatg gcgcgccgag aggctttaca
     2161 ctttatgctt ccggctggtc tcagcatcca cgcattataa cgtcgtatta gctgcctttc
     2221 gtcttcaata attcttgaca tccctatcag tgatagagat acttcgtgca atttttatat
     2281 caccgccagg ggtacaacaa gcaaagagga gaaatactag catgaacttg aaagataaga
     2341 ttttgggtgt tgctaaagag ctgtttatca aaaacggtta caatgctact accactggtg
     2401 agattgtgaa attgtctgaa tcttctaaag ggaatctata ctatcatttc aaaacgaagg
     2461 aaaatttgtt cttggaaatc ttgaacattg aagagagtaa atggcaagaa caatggaaaa
     2521 aggagcagat taaagctaaa actaatagag aaaagtttta tctgtataat gagttgtctt
     2581 tgactacaga gtattactat ccactacaga acgcaataat cgagttctac actgaatact
     2641 ataagactaa ctccatcaat gaaaaaatga acaaattaga gaataagtat atcgatgcat
     2701 atcacgttat tttcaaagaa ggtaatctta acggtgagtg gagtataaat gatgttaatg
     2761 ccgtttctaa aattgctgct aacgctgtta acggtattgt tacattcaca catgagcaaa
     2821 acatcaacga aagaattaag ttgatgaaca agttctctca gatctttttg aatggtctta
     2881 gtaaaggttc tggtcaccat catcaccacc attgataaac caggcatcaa ataaaacgaa
     2941 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     3001 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatagt cgggcacgac
     3061 tctcaagggc atcggtcgac acagaaaagc ccgcctttcg gcgggctttg ctcgagttat
     3121 tcaccagatg cttcattaga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     3181 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     3241 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     3301 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     3361 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     3421 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     3481 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     3541 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     3601 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     3661 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     3721 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     3781 caggatggga acaacgccag tgaaaagctc catggtatat ctccttctta aagttaaaca
     3841 aaattattgc ttttaagttc cctccaatcc ttatagaccg tgcgatcggt ctataaggat
     3901 tataatctat ttttttacat attacaacaa aaaatcttgc gaaatttaaa tgatgc
//
