LOCUS       pKB-PsspB-mKate2        8507 bp    DNA     circular SYN 22-MAR-2016
DEFINITION  Ligation of PsspB into pJWV102-mKate2*
ACCESSION   pKB-PsspB-mKate2
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_signal     179..221
                     /label=TER_rpsD
     misc_signal     complement(273..512)
                     /label=PssbB
     CDS             550..1242
                     /gene="mKate2"
     misc_signal     1317..1405
                     /label=TER_rrnB
     misc_signal     1410..1461
                     /label=TER_rpsI
     misc_signal     1466..1493
                     /label=TER_tufA
     CDS             1514..1558
                     /gene="'lacZ"
                     /product="promoterless and AUG-less 'lacZ"
     CDS             1623..2435
                     /gene="'bgaA"
                     /product="end of pneumo ß-Gal"
     CDS             complement(3664..4525)
                     /gene="bla"
                     /product="ß-lactamase"
     CDS             4734..5669
                     /gene="'gatC"
                     /product="EII galactitol"
     CDS             complement(6097..8031)
                     /gene="tetM"
                     /product="tetracycline resistance"
ORIGIN      
        1 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
       61 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
      121 atcatggcga ccacacccgt cctgtggatc tatcgatgca tgcagccggt accatcacaa
      181 gcactttggg acgttctccc ttagtgcttt tttgatttct cataggccgg ccgcctagaa
      241 ttccaatccc gggtaaagct tccagcggcc gcctcaggat attgcagaca cagtagtcta
      301 tgtgaccagt cagcctcgcc gtgttcagat tacagatatg accattatgg ccaatcaaca
      361 ggcgacaggt ttcatgattc ataaaaaata agaaatttcc tcgaaaagtt acaaatttct
      421 gtaacttttt ttgatttcct acgaatagat aagtaggagg aagaaaatat gtataataaa
      481 gttatcatga ttgggcgttt aacgtctaca ccggatccat tgatatcata ttctagagga
      541 ggaaaattaa tgtcagaact tatcaaggaa aatatgcaca tgaaattgta catggaagga
      601 acagtaaata atcaccactt taaatgtacc tcagaaggag aaggaaaacc atatgaaggt
      661 actcaaacca tgcgtattaa ggccgttgaa ggtggaccat tgccttttgc ctttgatatt
      721 cttgctacat cttttatgta cggatcaaag acttttatca atcataccca aggtatccca
      781 gatttcttta aacagtcatt tcctgaagga tttacatggg aacgtgtcac aacttatgaa
      841 gatggtggag tattgacagc aactcaagat acatctttgc aagatggttg tcttatctac
      901 aatgtaaaga tccgtggagt taattttcca agtaatggtc ctgttatgca gaaaaagacc
      961 cttggatggg aagcatctac cgaaacatta tatcctgccg atggtggatt ggaaggtcgt
     1021 gctgatatgg cattgaaact tgtcggtgga ggtcacctta tctgtaattt gaagaccaca
     1081 taccgttcta aaaagccagc taagaatctt aagatgcctg gtgtttacta cgtcgatcgt
     1141 cgtttagaac gtatcaaaga agcagataag gaaacttatg ttgaacagca cgaagtagcc
     1201 gtcgcacgtt attgtgattt gcctagtaaa ttgggacacc gttaataata aggatcctaa
     1261 ctcgagaaag aattcatgag atctcgcact agtcaaggtc ggcaattctg cagtactagg
     1321 acgccgccaa gccagcttaa acccagctca atgagctggg ttttttgttt gttaaaaatg
     1381 aagaagaaac tgtgaagcgt atttatagca aagcactcaa aagtttacct tatgggtgct
     1441 tttttcgtgc ttttttgaaa agacaaaaaa aagaaccttg ccaagcaaga ttcttttgat
     1501 agcgctatcg ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataata
     1561 ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt
     1621 ctggtgatga aatcaacgta acatttaaag ctgtcaaagc caaagtcatg agatggcgta
     1681 tggagcgtaa agctgacaag agcggtgttg cgatgattga gatgaccttc cttgcaccaa
     1741 gtgaattgcc tcaagaaagc actcaatcaa agattcttgt agatggaaaa gaacttgctg
     1801 atttcgctga aaatcgtcaa gactatcaaa ttacctataa aggtcaacgg ccaaaagtct
     1861 cagttgaaga aaacaatcaa gtagcttcaa ctgtggtaga tagtggagaa gatagccttc
     1921 cagtacttgt tcgcctcgtt tcagaaagtg gaaaacaagt caaggaatac cgtatccagt
     1981 tgactaagga aaaaccagtt tctgctgtac aagaagatct tccaaaactc gaatttgttg
     2041 aaaaagattt ggcctacaag acagttgaga aaaaagattc aacactgtat ctaggtgaaa
     2101 ctcgtgtaga acaagaagga aaagttggaa aagaacgtat ctttacagtg attaatcctg
     2161 atggaagtaa ggaagaaaaa ctccgtgaag tggtagaagt tccgacagac cgcatcgtct
     2221 tggttggaac caaaccagta gctcaagaag ctaaaaaacc acaagtgtca gaaaaagcag
     2281 atacaaaacc aattgattca agtgaagctg atcaaactaa taaagcccag ttaccaaata
     2341 caggtagtgc ggcaagccaa gcagcagtag cagcaggttt agctcttcta ggtttgagtg
     2401 caggattagt agttactaaa ggtaaaaaag aagactagct gcctcgcgcg tttcggtgat
     2461 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     2521 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     2581 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     2641 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     2701 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2761 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2821 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2881 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2941 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3001 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3061 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3121 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3181 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3241 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3301 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3361 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3421 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3481 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3541 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3601 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3661 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3721 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3781 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3841 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3901 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3961 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4021 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4081 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4141 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4201 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4261 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     4321 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4381 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4441 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4501 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4561 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4621 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4681 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattaattc
     4741 cttcttaacg ccccaagttc atcaccaatg acatcaactc acatgaacta catgatgaac
     4801 ccagttatca tggttttgga taagattttt gaaaaattct tcccaggcct tgataaatat
     4861 gactttgatg ctgctaaatt gaacaagaaa atcggtttct ggggatctaa attcttcatc
     4921 ggtttcatcc ttggtatcgt tatcggtatt atgggaactc cacatccaat tgcaggtgtt
     4981 gcagatgcag ataaatggcg tcttgttatc aaaggatggt tgtctcttgg tttgactgcc
     5041 ggtgtatctt tggaactctt ctcacttatc ggttcatggt tcatcgcagc cgtagaacca
     5101 ctatcacaag gtattacaaa cgttgctact aaacgtcttc aaggacgtaa attcaatatc
     5161 ggtcttgact ggccattcat cgctggtcgt gctgaaatct gggcttgtgc caacgtactt
     5221 gcaccaatca tgttgattga agcagtgctt ctttcaaaag ttggaaatgg tatcttgcca
     5281 cttgcaggta tcatcgctat gggtgttact ccagctctct tggttgtaac tcgtggtaaa
     5341 ttgctccgta tgattatctt cggaacactc ttgttgccac tcttccttct ttcaggtaca
     5401 cttattgcac catttgcaac agaacttgct aaaggtgtag gtgccttccc agaaggtgtg
     5461 agccaaactc aattgattac tcactctact cttgaaggac caatcgaaaa acttcttggt
     5521 tggacaattg gtaacactac aactggtgat atcaaagcaa tccttggtgc agtagccttc
     5581 cttgtattct atatcggtat ctttgcttgg tacagaaaac aaatgatcaa acgtaacgaa
     5641 gagtacgcag caaaagcaaa ataatgcgct ccgctagctt tacagacaaa gaactatcct
     5701 taatggaagc actagcaaaa ggtatcaatg aagcaagaaa tattgaaggc tagtcagtaa
     5761 aattcagata aacaaaaaga gccgataaga tgagagtttc atttctcaaa ttatcggctc
     5821 tgcgtcttgg cgtctggctc tttgattgtt attatattca ttttttgaac attaattaaa
     5881 gtttcgttct tacatttggg gcagtataaa gggaaatttt taagtatagt atccactcgt
     5941 attcgtagtc ttgttttatt tccacaaata ggacacaata tccacttgta gtttataata
     6001 acaatctcct cctttccact ttaattcaaa tctatattaa agaatatttc atcttattta
     6061 ataagaaacc atatttatat aacaacataa aacgcactaa gttattttat tgaacatata
     6121 tcttacttta tctatctgac tatttagacg acgggtctgg caaacaggtt cgccagtggt
     6181 aacctgatat ccttttagct ctgctaaaca aacactaagc ccatttgtaa aaaaagttaa
     6241 atcattgcga taatcttgaa tacatcgagc aggaatttct ccaataataa tgacctcatt
     6301 atttttcagt tgagtattta cgatatttgc acaatatttg ggagcatcgt tatatgcccg
     6361 tgaaagatat tcctgtggtg cataaacttt aaaactaaga tatggctcta acaattctgt
     6421 tccagctttt ctaaaggctt gctccaatac aataggagca agcatccgaa aatctgctgg
     6481 ggtactaaca gggctatagt ataagccata cttaaaacag attttacagt ccgtcacatt
     6541 ccaaccatac aatccttgtt cacaaccata gcgtatccct tccataactg cattttgaaa
     6601 tgattgattt aagtatccaa gagaaaccga gctctcatac tgcattccac ttcccaacgg
     6661 aagcggtgat acagataaac caatggaagc ccagaaagga tttggcggca cttcgatgtg
     6721 aatggtatat tctgcatttt ttaacggtct ctccatataa atgactgtag gctcttttag
     6781 ttctatctcc acatgatact tttcttgcaa cagtgcacta atcacttcca tttgtacttt
     6841 ccctaagaaa gaaagtataa tttcatgtgt cgtagaatcc acgtaatatc gtagaagcgg
     6901 atcactatct gagatttcca aaagggcatc aagcaacatt tctctctgtt caggtttact
     6961 cggttcaaca gttgtttgta gtagagggtg cggattttca atcttttttc tctgtggcaa
     7021 tagttttgta tctccaagaa cactatttaa cttcaaaaac tcattttgca aaataacaat
     7081 ttctccagaa taagctctat caatcttaca taattcacca tttattgaag tatacatttc
     7141 tgtaactttt attttttctt tttctgatac tctaaccgaa tctcgtaaat gtagtactcc
     7201 actataaagg cgtatatatg caagacgttg tctttttttt gtatattcaa ttttgaaaac
     7261 atttccgcaa agttcagacg gacctcgatg tgttgatgaa taaaatttat tagtaataac
     7321 ttctataagg ttatcaatcc ctatattact ttttgcactt ccatgataaa gagggaacag
     7381 agaacaattc tgaaatctta tgctttcctc ttgttcgagt tccaatgctt ctaatgattt
     7441 accggacata tatttctcta aaaggtcatc gtttccctct attaccgtat cccattgttc
     7501 agattcggta aagttcgtca cacacacatt aggatacagt tctaccttct gtttgattac
     7561 aatttcggca gaaagtttct ctttaatatc ctgataaacc gttgataaat caattccatt
     7621 ttggtcaatc ttattgataa aaaagattgt gggaatcccc attttcctaa gtgcatgaaa
     7681 taatatacga gtttgtgctt gtacgccatc ttttgcagaa atcagtagaa ttgccccatc
     7741 taaaactgat aatgaacgat atacttctgc taagaaatcc atatgtcctg gcgtgtctat
     7801 gatgttcacc ttcgtatttt cccactgaaa agaggttatt cctgtctgaa ttgtaattcc
     7861 tctctgacgt tctaaaagcg tattatccgt cctcgttgta cctttgtcca cgcttcctaa
     7921 ttctgtaatc gctccactgt tatataataa gctttctgtt aaggtagttt ttcctgcatc
     7981 aacatgagct aaaactccaa tattaataat tttcatgtga ttttcctcca ttcaaaagcc
     8041 caaaagggca taaaaatccc agtgataaat actcttatca ctgggatttt tatgcataac
     8101 cataggcata caaagcatac agatattctc cggatacttt agaatcacat gataaaggta
     8161 ttcttaaact gggtacaaaa aactaagccc tcctaaaaaa ggacatccaa ttatttgttc
     8221 ccgctatcaa attgacagtt tatttaagaa taccttgccg catatttatt aactcctttt
     8281 aaatagatac ttaaattata gcacgtaaga gcatatttgt aaaggaatct ccaatttttt
     8341 atcaaaaaga gtacatgatt acaaagtatc tgtaatcatg taccaatatt tgttatttta
     8401 caatcttcca attatcttgt ttttctaata tcaactcaaa ttgcgagatt tgggttgcct
     8461 ttgtttcttg atctaaatat ttcacagcga cattcacgat tacttgg
//
