LOCUS       Exported                5017 bp ds-DNA    circular SYN 10-AGO-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pT3-Neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Marco
  TITLE     Direct Submission
  JOURNAL   Exported segunda-feira, 17 de ago de 2015 from SnapGene Viewer 2.2.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    6..359
                     /note="IR/DR"
     primer_bind     complement(404..420)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    428..444
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(452..482)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    497..518
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(806..1394)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1565..2425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2426..2530)
                     /gene="bla"
                     /note="AmpR promoter"
     primer_bind     3004..3020
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3040..3393
                     /note="IR/DR"
     promoter        3518..3903
                     /note="U3 MSCV"
     CDS             3914..4708
                     /codon_start=1
                     /note="Neo"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4743..4761)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    4787..5011
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gatccccttg aaatacatcc acaggtacac ctccaattga ctcaaatgat gtcaattagt
       61 ctatcagaag cttctaaagc catgacatca ttttctggaa ttttccaagc tgtttaaagg
      121 cacagtcaac ttagtgtatg taaacttctg acccactgga attgtgatac agtgaattat
      181 aagtgaaata atctgtctgt aaacaattgt tggaaaaatg acttgtgtca tgcacaaagt
      241 agatgtccta actgacttgc caaaactatt gtttgttaac aagaaatttg tggagtagtt
      301 gaaaaacgag ttttaatgac tccaacttaa gtgtatgtaa acttccgact tcaactgtat
      361 atctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
      421 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      481 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      541 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      601 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     1021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     1261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2521 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2581 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2641 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2701 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2761 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2821 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2881 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2941 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     3001 gttgtaaaac gacggccagt gaattcgagc tcggtaccct acagttgaag tcggaagttt
     3061 acatacactt aagttggagt cattaaaact cgtttttcaa ctactccaca aatttcttgt
     3121 taacaaacaa tagttttggc aagtcagtta ggacatctac tttgtgcatg acacaagtca
     3181 tttttccaac aattgtttac agacagatta tttcacttat aattcactgt atcacaattc
     3241 cagtgggtca gaagtttaca tacactaagt tgactgtgcc tttaaacagc ttggaaaatt
     3301 ccagaaaatg atgtcatggc tttagaagct tctgatagac taattgacat catttgagtc
     3361 aattggaggt gtacctgtgg atgtatttca agggaattct gtggaatgtg tgtcagttag
     3421 ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatatcgat
     3481 actagtttaa ttaagatctc gagcacgaga ctagccgtcg agggaatgaa agaccccacc
     3541 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac
     3601 tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa
     3661 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     3721 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     3781 ggacctgaaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     3841 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg
     3901 ccgatccacc ggtatggccg aacaggatgg actgcacgca ggctcccccg ccgcttgggt
     3961 ggaaagactg tttggatacg attgggctca gcagactatt ggatgctctg acgcagccgt
     4021 gttccggctg agtgctcagg ggcgccccgt gctgtttgtc aagacagacc tgtcaggagc
     4081 actgaacgag ctgcaggatg aagcagcacg actgagctgg ctggcaacca caggagtgcc
     4141 ttgtgctgca gtcctggacg tggtcactga ggcaggacga gattggctgc tgctgggaga
     4201 agtgccagga caggacctgc tgagctccca cctggcacca gctgagaagg tctccatcat
     4261 ggcagatgcc atgcggagac tgcatactct ggaccccgcc acctgccctt tcgatcacca
     4321 ggctaaacat cgcattgaga gggctcgcac ccgaatggaa gcaggcctgg tggaccagga
     4381 cgatctggat gaggaacacc aggggctggc tcctgcagag ctgtttgcac ggctgaaagc
     4441 cagaatgcca gacggagaag atctggtggt cacccatggc gacgcttgcc tgcccaacat
     4501 catggtggag aatggcagat tcagtgggtt tattgattgt ggacgactgg gcgtcgccga
     4561 cagataccag gatatcgccc tggctacaag agacattgca gaggaactgg gcggggaatg
     4621 ggccgatagg ttcctggtgc tgtatggcat cgctgcccct gattcacaga ggattgcctt
     4681 ttacagactg ctggatgagt tcttttgagc ggccgctcga gcatgcatct agagggccct
     4741 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
     4801 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4861 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4921 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4981 aggcatgctg gggatgcggt gggctctatg gcttctg
//
