LOCUS Exported 12486 bp ds-DNA circular SYN 21-OCT-2015 DEFINITION Ligation of lentiCRISPRv2 mNeon* into pL40C-CRISPR.EFS.mNeon*. ACCESSION . VERSION . KEYWORDS pL40C-CRISPR.EFS.mNeon NL SL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12486) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 21, 2015 from SnapGene Viewer 2.6.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12486 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 430..811 /note="CMV IE enhancer" misc_feature 826..1058 /note="SV40 enhancer" misc_feature 1075..1487 /note="RSV/5'LTR" /note="LTR" misc_feature 1538..1675 /note="psi" misc_feature 1642..1996 /note="gag'" misc_feature 2911..3028 /note="cPPT" misc_feature 3079..3327 /note="U6 Prom" misc_feature 3355..3446 /note="sgRNA" misc_feature 3359 /note="mod T-A" misc_feature 3367..3371 /note="elong stem" misc_feature 3376..3380 /note="elong stem comp" misc_feature 3390 /note="mod A-T" misc_feature 3469..3713 /note="EFS" misc_feature 3729..7953 /note="SpCas9'" misc_feature 7959..8027 /note="P2A" misc_feature 8028..8738 /note="mNeonGreen" misc_feature 8754..9342 /note="WPRE" /note="misc_RNA" misc_feature 9413..9648 /note="3'\LTR\SIN" /note="LTR" misc_feature 9725..9855 /note="SV40\polyA" /note="polyA_signal" misc_feature 10202..10632 /note="f1\Ori" /note="rep_origin" misc_feature 11003..11809 /note="AmpR" misc_feature complement(11942..64) /note="pUC\Ori" /note="rep_origin" ORIGIN 1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc 421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat 481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg 541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa 601 cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact 661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta 721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt 781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct 841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt 901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg 1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc 1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt 1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg 1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta 1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca 1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg 1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta 1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta 1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac 1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa 1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag 1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag 1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga 1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat 2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 2761 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 2821 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt 2881 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta 2941 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca 3001 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt 3061 ttggttaatt aaggtaccga gggcctattt cccatgattc cttcatattt gcatatacga 3121 tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta 3181 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt 3241 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta 3301 tatatcttgt ggaaaggacg aaacaccgga gacgtatgtc gacaactcgt ctctgtttaa 3361 gagctatgct ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa 3421 aagtggcacc gagtcggtgc ttttttgaat tctcctctag aactcgagtg gctccggtgc 3481 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg 3541 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta 3601 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt 3661 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg 3721 ccaccatgga ttacaaagac gatgacgata agatggcccc aaagaagaag cggaaggtcg 3781 gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggac atcggcacca 3841 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg 3901 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg 3961 acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca 4021 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg 4081 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg 4141 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca 4201 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga 4261 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc 4321 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc 4381 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg 4441 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga 4501 agaatggcct gttcggcaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga 4561 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg 4621 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca 4681 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca 4741 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc 4801 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc 4861 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca 4921 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga 4981 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga 5041 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga 5101 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc 5161 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca 5221 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc 5281 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc 5341 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa 5401 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca 5461 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt 5521 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat 5581 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg 5641 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg 5701 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc 5761 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc 5821 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca 5881 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt 5941 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta 6001 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc 6061 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac 6121 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc 6181 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt 6241 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt 6301 ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca 6361 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag 6421 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc 6481 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg 6541 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga 6601 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga 6661 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca 6721 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg 6781 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca 6841 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg 6901 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca 6961 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt 7021 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata 7081 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga 7141 ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct 7201 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt 7261 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct 7321 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc 7381 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc 7441 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga 7501 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc 7561 agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca 7621 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct 7681 acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta 7741 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga 7801 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg 7861 gcctgtacga gacacggatc gacctgtctc agctgggagg cgacaagcgt cctgctgcta 7921 ctaagaaagc tggtcaagct aagaaaaaga aagctagcgg aggaagcggc gccaccaatt 7981 tcagcctgct gaaacaggcc ggcgacgtgg aagagaaccc tggccctatg gtgtccaagg 8041 gcgaagagga caacatggcc agcctgcctg ccacccacga gctgcacatc ttcggcagca 8101 tcaacggcgt ggacttcgac atggtgggac agggcaccgg caaccccaac gacggctacg 8161 aggaactgaa cctgaagtcc acaaagggcg acctgcagtt cagcccctgg attctggtgc 8221 cccacatcgg ctacggcttc caccagtacc tgccctaccc cgacggcatg agccctttcc 8281 aagccgccat ggtggatggc agcggctacc aggtgcaccg gaccatgcag tttgaggacg 8341 gcgccagcct gaccgtgaac tacaggtaca catacgaggg cagccacatc aagggcgagg 8401 cccaagtgaa gggcacaggc tttccagccg acggccccgt gatgaccaat agcctgacag 8461 ccgccgactg gtgcagaagc aagaaaacct accccaatga caagaccatc atcagcacct 8521 tcaagtggtc ctacaccacc ggcaatggca agcggtacag aagcaccgcc cggaccacct 8581 acaccttcgc caaacccatg gccgccaact acctgaagaa ccagcctatg tacgtgttcc 8641 gcaagaccga gctgaagcac tccaagacag aactgaactt caaagagtgg cagaaagcct 8701 tcaccgacgt gatgggcatg gacgagctgt acaagtgaac gcgttaagtc gacaatcaac 8761 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 8821 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 8881 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 8941 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 9001 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 9061 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 9121 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 9181 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 9241 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 9301 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat 9361 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 9421 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt 9481 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 9541 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 9601 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag 9661 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 9721 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 9781 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 9841 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact 9901 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 9961 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 10021 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg 10081 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 10141 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 10201 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 10261 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 10321 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 10381 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 10441 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 10501 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 10561 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 10621 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 10681 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 10741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 10801 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 10861 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 10921 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 10981 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 11041 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 11101 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 11161 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 11221 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 11281 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 11341 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 11401 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 11461 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 11521 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 11581 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 11641 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 11701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 11761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 11821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 11881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 11941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 12001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 12061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 12121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 12181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 12241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 12301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 12361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 12421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 12481 ttttgc //