LOCUS pBS_XTR_f1 7776 bp ds-DNA circular SYN 05-Oct-2008 DEFINITION - ACCESSION - KEYWORDS - SOURCE - FEATURES Location/Qualifiers rep_origin complement(3..458) /note="f1 ORI" primer_bind 600..615 /note="M13 forward" promoter 626..645 /note="T7" misc_feature <658..682 /note="linearization cassette" gene complement(1093..1986) /note="DTA Gene" promoter complement(1765..2271) /note="Ppgk" misc_recomb 2290..2322 /note="5' MCS" misc_recomb 2349..2382 /note="Frt" promoter 2440..2954 /note="PGK" promoter 2956..3022 /note="EM7" gene 3023..3826 /note="Neo" terminator 3840..4142 /note="bGHpA" misc_recomb 4143..4176 /note="Frt" misc_recomb 4201..4234 /note="2272LoxP" misc_recomb 4249..4282 /note="5171LoxP" polyA_signal complement(4329..4558) /note="SV40pA" gene complement(4580..5299) /note="GFP" exon 5300 /note="G f2" intron 5301..5302 /note="intron/exon boundary" intron complement(5303..5314) /note="polyY track" misc_recomb complement(5380..5413) /note="2272LoxP" misc_recomb complement(5460..5493) /note="5171LoxP" misc_recomb 5499..5532 /note="Frt" misc_recomb 5533..5558 /note="3'MCS (PacI/AatII/AflII/AvrII)" primer_bind complement(5627..5648) /note="pUC/M13 reverse sequencing primer" promoter complement(5632..5753) /note="Plac" rep_origin 6011..6040 /note="ColE1-derived plasmid replication origin" rep_origin complement(6029..6617) /note="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" CDS complement(6788..7648) /codon_start=1 /db_xref="GI:455370" /gene="bla" /note="E-286" /product="beta-lactamase" /protein_id="AAB59737.1" /transl_table=11 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" BASE COUNT 1809 A 2022 C 2055 G 1888 T 0 OTHER ORIGIN ? 1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG 481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG 541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCAT 661 TTAAATGTTT AAACGGTTAC CTCTAGATGC ATGCTCGAGC CCCAGCTGGT TCTTTCCGCC 721 TCAGAAGCCA TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC 781 CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC 841 GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG 901 GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT 961 GATCAGCGAG CTCTAGGATC TGCATTCCAC CACTGCTCCC ATTCATCAGT TCCATAGGTT 1021 GGAATCTAAA ATACACAAAC AATTAGAATC AGTAGTTTAA CACATTATAC ACTTAAAAAT 1081 TTTATATTTA CCTTAGAGCT TTAAATCTCT GTAGGTAGTT TGTCCAATTA TGTCACACCA 1141 CAGAAGTAAG GTTCCTTCAC AAAGAGATCG CCTGACACGA TTTCCTGCAC AGGCTTGAGC 1201 CATATACTCA TACATCGCAT CTTGGCCACG TTTTCCACGG GTTTCAAAAT TAATCTCAAG 1261 TTCTACGCTT AACGCTTTCG CCTGTTCCCA GTTATTAATA TATTCAACGC TAGAACTCCC 1321 CTCAGCGAAG GGAAGGCTGA GCACTACACG CGAAGCACCA TCACCGAACC TTTTGATAAA 1381 CTCTTCCGTT CCGACTTGCT CCATCAACGG TTCAGTGAGA CTTAAACCTA ACTCTTTCTT 1441 AATAGTTTCG GCATTATCCA CTTTTAGTGC GAGAACCTTC GTCAGTCCTG GATACGTCAC 1501 TTTGACCACG CCTCCAGCTT TTCCAGAGAG CGGGTTTTCA TTATCTACAG AGTATCCCGC 1561 AGCGTCGTAT TTATTGTCGG TACTATAAAA CCCTTTCCAA TCATCGTCAT AATTTCCTTG 1621 TGTACCAGAT TTTGGCTTTT GTATACCTTT TTGAATGGAA TCTACATAAC CAGGTTTAGT 1681 CCCGTGGTAC GAAGAAAAGT TTTCCATCAC AAAAGATTTA GAAGAATCAA CAACATCATC 1741 AGGATCCATG GCGAGGACCT GCAGGTCGAA AGGCCCGGAG ATGAGGAAGA GGAGAACAGC 1801 GCGGCAGACG TGCGCTTTTG AAGCGTGCAG AATGCCGGGC CTCCGGAGGA CCTTCGGGCG 1861 CCCGCCCCGC CCCTGAGCCC GCCCCTGAGC CCGCCCCCGG ACCCACCCCT TCCCAGCCTC 1921 TGAGCCCAGA AAGCGAAGGA GCAAAGCTGC TATTGGCCGC TGCCCCAAAG GCCTACCCGC 1981 TTCCATTGCT CAGCGGTGCT GTCCATCTGC ACGAGACTAG TGAGACGTGC TACTTCCATT 2041 TGTCACGTCC TGCACGACGC GAGCTGCGGG GCGGGGGGGA ACTTCCTGAC TAGGGGAGGA 2101 GTAGAAGGTG GCGCGAAGGG GCCACCAAAG AACGGAGCCG GTTGGCGCCT ACCGGTGGAT 2161 GTGGAATGTG TGCGAGGCCA GAGGCCACTT GTGTAGCGCC AAGTGCCCAG CGGGGCTGCT 2221 AAAGCGCATG CTCCAGACTG CCTTGGGAAA AGCGCCTCCC CTACCCGGTA GAATTCGGTC 2281 GACGGATCCG GCGCGCCGCG ATCGCGGCCG GCCGCGGCCG CGTATCGATA AGCTTGATAT 2341 CGAATTCCGA AGTTCCTATT CTCTAGAAAG TATAGGAACT TCAGGTCTGA AGAGGAGTTT 2401 ACGTCCAGCC AAGCTAGCTT GGCTGCAGGT CGTCGAAATT CTACCGGGTA GGGGAGGCGC 2461 TTTTCCCAAG GCAGTCTGGA GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA 2521 CAAGTGGCCT CTGGCCTCGC ACACATTCCA CATCCACCGG TAGGCGCCAA CCGGCTCCGT 2581 TCTTTGGTGG CCCCTTCGCG CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG 2641 CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG 2701 TGCAGATGGA CAGCACCGCT GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT 2761 AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG 2821 GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT 2881 TCTGCACGCT TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC 2941 TTTCGACCTG CAGCCTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA 3001 CGACAAGGTG AGGAACTAAA CCATGGGATC GGCCATTGAA CAAGATGGAT TGCACGCAGG 3061 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 3121 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 3181 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 3241 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 3301 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 3361 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 3421 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 3481 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 3541 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGATGAT CTCGTCGTGA CCCATGGCGA 3601 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 3661 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 3721 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 3781 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGGGG ATCAATTCTC 3841 TAGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC 3901 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA 3961 TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG 4021 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG 4081 CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCG ACTAGAGCTT GCGGAACCCT 4141 TCGAAGTTCC TATTCTCTAG AAAGTATAGG AACTTCATCA GTCAGGTACA TAATGGATCC 4201 ATAACTTCGT ATAGGATACC TTATACGAAG TTATCTCAGC TAGAAGCCAT AACTTCGTAT 4261 AGTACACATT ATACGAAGTT ATGTTTATCG AATTCCTGCA GCCCGGTAGA TCTTTACAAT 4321 TTACGCGTTA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA 4381 ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA 4441 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG 4501 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT 4561 ATCTAGATCC GGTGGATCCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG 4621 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG 4681 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG 4741 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT 4801 TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT 4861 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT 4921 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT 4981 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG 5041 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT 5101 AGGTCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA 5161 ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT 5221 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT 5281 CCTCGCCCTT GCTCACCATC CTGGAAAAAA GAAATGCGAA GATTGCGCCT GCGCATCGAT 5341 AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGCCCGGA TAACTTCGTA TAAGGTATCC 5401 TATACGAAGT TATCCATTCA GGCTGTGCTA GCATCAATGG CATGGCACAA AGCTTAGCCA 5461 TAACTTCGTA TAATGTGTAC TATACGAAGT TATCCCGGGA AGTTCCTATT CTCTAGAAAG 5521 TATAGGAACT TCTTAATTAA GACGTCCTTA AGCCTAGGCC ACCGCGGTGG AGCTCCAGCT 5581 TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 5641 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 5701 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 5761 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 5821 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 5881 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 5941 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 6001 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 6061 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 6121 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 6181 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 6241 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 6301 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 6361 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 6421 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 6481 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 6541 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 6601 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 6661 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 6721 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 6781 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 6841 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 6901 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 6961 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 7021 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 7081 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 7141 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 7201 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 7261 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 7321 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 7381 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 7441 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 7501 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 7561 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 7621 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 7681 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 7741 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCAC //