LOCUS       pBS_XTR_f1              7776 bp ds-DNA     circular SYN 05-Oct-2008
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     rep_origin      complement(3..458)
                     /note="f1 ORI"
     primer_bind     600..615
                     /note="M13 forward"
     promoter        626..645
                     /note="T7"
     misc_feature    <658..682
                     /note="linearization cassette"
     gene            complement(1093..1986)
                     /note="DTA Gene"
     promoter        complement(1765..2271)
                     /note="Ppgk"
     misc_recomb     2290..2322
                     /note="5' MCS"
     misc_recomb     2349..2382
                     /note="Frt"
     promoter        2440..2954
                     /note="PGK"
     promoter        2956..3022
                     /note="EM7"
     gene            3023..3826
                     /note="Neo"
     terminator      3840..4142
                     /note="bGHpA"
     misc_recomb     4143..4176
                     /note="Frt"
     misc_recomb     4201..4234
                     /note="2272LoxP"
     misc_recomb     4249..4282
                     /note="5171LoxP"
     polyA_signal    complement(4329..4558)
                     /note="SV40pA"
     gene            complement(4580..5299)
                     /note="GFP"
     exon            5300
                     /note="G f2"
     intron          5301..5302
                     /note="intron/exon boundary"
     intron          complement(5303..5314)
                     /note="polyY track"
     misc_recomb     complement(5380..5413)
                     /note="2272LoxP"
     misc_recomb     complement(5460..5493)
                     /note="5171LoxP"
     misc_recomb     5499..5532
                     /note="Frt"
     misc_recomb     5533..5558
                     /note="3'MCS (PacI/AatII/AflII/AvrII)"
     primer_bind     complement(5627..5648)
                     /note="pUC/M13 reverse sequencing primer"
     promoter        complement(5632..5753)
                     /note="Plac"
     rep_origin      6011..6040
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      complement(6029..6617)
                     /note="pMB1 origin of replication (clockwise) (RNAII -35
                     to RNA/DNA switch point)"
     CDS             complement(6788..7648)
                     /codon_start=1
                     /db_xref="GI:455370"
                     /gene="bla"
                     /note="E-286"
                     /product="beta-lactamase"
                     /protein_id="AAB59737.1"
                     /transl_table=11
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
BASE COUNT      1809 A      2022 C      2055 G      1888 T         0 OTHER
ORIGIN      ?
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCAT
      661 TTAAATGTTT AAACGGTTAC CTCTAGATGC ATGCTCGAGC CCCAGCTGGT TCTTTCCGCC
      721 TCAGAAGCCA TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC
      781 CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC
      841 GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG
      901 GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT
      961 GATCAGCGAG CTCTAGGATC TGCATTCCAC CACTGCTCCC ATTCATCAGT TCCATAGGTT
     1021 GGAATCTAAA ATACACAAAC AATTAGAATC AGTAGTTTAA CACATTATAC ACTTAAAAAT
     1081 TTTATATTTA CCTTAGAGCT TTAAATCTCT GTAGGTAGTT TGTCCAATTA TGTCACACCA
     1141 CAGAAGTAAG GTTCCTTCAC AAAGAGATCG CCTGACACGA TTTCCTGCAC AGGCTTGAGC
     1201 CATATACTCA TACATCGCAT CTTGGCCACG TTTTCCACGG GTTTCAAAAT TAATCTCAAG
     1261 TTCTACGCTT AACGCTTTCG CCTGTTCCCA GTTATTAATA TATTCAACGC TAGAACTCCC
     1321 CTCAGCGAAG GGAAGGCTGA GCACTACACG CGAAGCACCA TCACCGAACC TTTTGATAAA
     1381 CTCTTCCGTT CCGACTTGCT CCATCAACGG TTCAGTGAGA CTTAAACCTA ACTCTTTCTT
     1441 AATAGTTTCG GCATTATCCA CTTTTAGTGC GAGAACCTTC GTCAGTCCTG GATACGTCAC
     1501 TTTGACCACG CCTCCAGCTT TTCCAGAGAG CGGGTTTTCA TTATCTACAG AGTATCCCGC
     1561 AGCGTCGTAT TTATTGTCGG TACTATAAAA CCCTTTCCAA TCATCGTCAT AATTTCCTTG
     1621 TGTACCAGAT TTTGGCTTTT GTATACCTTT TTGAATGGAA TCTACATAAC CAGGTTTAGT
     1681 CCCGTGGTAC GAAGAAAAGT TTTCCATCAC AAAAGATTTA GAAGAATCAA CAACATCATC
     1741 AGGATCCATG GCGAGGACCT GCAGGTCGAA AGGCCCGGAG ATGAGGAAGA GGAGAACAGC
     1801 GCGGCAGACG TGCGCTTTTG AAGCGTGCAG AATGCCGGGC CTCCGGAGGA CCTTCGGGCG
     1861 CCCGCCCCGC CCCTGAGCCC GCCCCTGAGC CCGCCCCCGG ACCCACCCCT TCCCAGCCTC
     1921 TGAGCCCAGA AAGCGAAGGA GCAAAGCTGC TATTGGCCGC TGCCCCAAAG GCCTACCCGC
     1981 TTCCATTGCT CAGCGGTGCT GTCCATCTGC ACGAGACTAG TGAGACGTGC TACTTCCATT
     2041 TGTCACGTCC TGCACGACGC GAGCTGCGGG GCGGGGGGGA ACTTCCTGAC TAGGGGAGGA
     2101 GTAGAAGGTG GCGCGAAGGG GCCACCAAAG AACGGAGCCG GTTGGCGCCT ACCGGTGGAT
     2161 GTGGAATGTG TGCGAGGCCA GAGGCCACTT GTGTAGCGCC AAGTGCCCAG CGGGGCTGCT
     2221 AAAGCGCATG CTCCAGACTG CCTTGGGAAA AGCGCCTCCC CTACCCGGTA GAATTCGGTC
     2281 GACGGATCCG GCGCGCCGCG ATCGCGGCCG GCCGCGGCCG CGTATCGATA AGCTTGATAT
     2341 CGAATTCCGA AGTTCCTATT CTCTAGAAAG TATAGGAACT TCAGGTCTGA AGAGGAGTTT
     2401 ACGTCCAGCC AAGCTAGCTT GGCTGCAGGT CGTCGAAATT CTACCGGGTA GGGGAGGCGC
     2461 TTTTCCCAAG GCAGTCTGGA GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA
     2521 CAAGTGGCCT CTGGCCTCGC ACACATTCCA CATCCACCGG TAGGCGCCAA CCGGCTCCGT
     2581 TCTTTGGTGG CCCCTTCGCG CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG
     2641 CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG
     2701 TGCAGATGGA CAGCACCGCT GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT
     2761 AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG
     2821 GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT
     2881 TCTGCACGCT TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC
     2941 TTTCGACCTG CAGCCTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA
     3001 CGACAAGGTG AGGAACTAAA CCATGGGATC GGCCATTGAA CAAGATGGAT TGCACGCAGG
     3061 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
     3121 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
     3181 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
     3241 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
     3301 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
     3361 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
     3421 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
     3481 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
     3541 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGATGAT CTCGTCGTGA CCCATGGCGA
     3601 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
     3661 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
     3721 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA
     3781 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGGGG ATCAATTCTC
     3841 TAGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC
     3901 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA
     3961 TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG
     4021 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG
     4081 CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCG ACTAGAGCTT GCGGAACCCT
     4141 TCGAAGTTCC TATTCTCTAG AAAGTATAGG AACTTCATCA GTCAGGTACA TAATGGATCC
     4201 ATAACTTCGT ATAGGATACC TTATACGAAG TTATCTCAGC TAGAAGCCAT AACTTCGTAT
     4261 AGTACACATT ATACGAAGTT ATGTTTATCG AATTCCTGCA GCCCGGTAGA TCTTTACAAT
     4321 TTACGCGTTA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
     4381 ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA
     4441 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG
     4501 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT
     4561 ATCTAGATCC GGTGGATCCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
     4621 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
     4681 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
     4741 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT
     4801 TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT
     4861 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
     4921 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
     4981 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
     5041 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT
     5101 AGGTCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA
     5161 ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT
     5221 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT
     5281 CCTCGCCCTT GCTCACCATC CTGGAAAAAA GAAATGCGAA GATTGCGCCT GCGCATCGAT
     5341 AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGCCCGGA TAACTTCGTA TAAGGTATCC
     5401 TATACGAAGT TATCCATTCA GGCTGTGCTA GCATCAATGG CATGGCACAA AGCTTAGCCA
     5461 TAACTTCGTA TAATGTGTAC TATACGAAGT TATCCCGGGA AGTTCCTATT CTCTAGAAAG
     5521 TATAGGAACT TCTTAATTAA GACGTCCTTA AGCCTAGGCC ACCGCGGTGG AGCTCCAGCT
     5581 TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC
     5641 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT
     5701 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC
     5761 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG
     5821 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
     5881 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
     5941 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
     6001 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
     6061 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
     6121 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
     6181 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
     6241 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
     6301 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
     6361 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
     6421 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG
     6481 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
     6541 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     6601 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
     6661 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
     6721 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
     6781 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
     6841 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
     6901 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG
     6961 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
     7021 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
     7081 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG
     7141 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA
     7201 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT
     7261 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT
     7321 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC
     7381 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA
     7441 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT
     7501 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT
     7561 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA
     7621 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT
     7681 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA
     7741 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCAC
//
