LOCUS       pYS7                    2804 bp    DNA     circular      5-MAY-2008
DEFINITION  pYS7 (TetO2-CYC1TATA ENTRY clone).
KEYWORDS    Yasu; GATEWAY cloning; TetO.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|481562471|
COMMENT     VNTDBDATE|481563109|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pYS7|
COMMENT     VNTAUTHORNAME|Yasu|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     terminator      complement(268..295)
                     /vntifkey="43"
                     /label=T2
                     /note="rrnB T2 transcription termination sequence"
     terminator      complement(427..470)
                     /vntifkey="43"
                     /label=T1
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     537..552
                     /vntifkey="28"
                     /label=M13\Forward
                     /note="M13 forward (-20) priming site"
     primer_bind     complement(1069..1085)
                     /vntifkey="28"
                     /label=M13\Reverse
                     /note="M13 reverse priming site"
     misc_recomb     570..665
                     /vntifkey="86"
                     /label=attL5
                     /note="attL5 recombination site"
     misc_recomb     919..1014
                     /vntifkey="86"
                     /label=attL2
                     /note="attL2 recombination site"
     CDS             1189..1998
                     /vntifkey="4"
                     /label=Kan
                     /note="Kanamycin resistance gene"
     rep_origin      2119..2792
                     /vntifkey="33"
                     /label=pUC\ori
                     /note="pUC replication origin"
     misc_feature    complement(666..805)
                     /vntifkey="21"
                     /label=CYC1TATA
                     /note="CYC1TATA sequence"
     misc_binding    complement(806..910)
                     /vntifkey="20"
                     /label=TetO2
                     /note="TetO2"
BASE COUNT      724 a       644 c       684 g       752 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa 
      661 agttgtaatt tagtgtgtgt atttgtgttt gcgtgtctat agaagtatag taatttatgc 
      721 tacaaaggac ctaatgtata aggaaagaat atttagagaa aagaagaaaa caagagtttt 
      781 atatacatac agagcacatg catgcagtgg tctcgatcga gaaactcgac tttgtcgact 
      841 ctctatcact gatagggatc gagaaactcg actttgtcga ctctctatca ctgataggga 
      901 tcgaggaatt gatctgccca gctttcttgt acaaagttgg cattataaga aagcattgct 
      961 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc 
     1021 cagctgcagg gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt 
     1081 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat 
     1141 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg 
     1201 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta 
     1261 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg 
     1321 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat 
     1381 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc 
     1441 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga 
     1501 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg 
     1561 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc 
     1621 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg 
     1681 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat 
     1741 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 
     1801 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 
     1861 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 
     1921 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 
     1981 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag 
     2041 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt 
     2101 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 
     2161 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 
     2221 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 
     2281 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 
     2341 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 
     2401 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 
     2461 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 
     2521 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 
     2581 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 
     2641 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 
     2701 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 
     2761 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt 
//
