LOCUS       pHD4                    3099 bp    DNA     circular     10-DEC-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|562784842|
COMMENT     VNTDBDATE|573229314|
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COMMENT                                   -10 7.14325 1.04901 #145#) 
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COMMENT                                   -10 7.24316 1.04901 #147#) 
COMMENT                             #149=(CScratch 
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FEATURES             Location/Qualifiers
     misc_feature    272..1462
                     /dnas_title="TetR"
                     /vntifkey="21"
                     /label=TetR
     promoter        229..235
                     /dnas_title="Tet_promoter"
                     /vntifkey="30"
                     /label=Tet_promoter
     primer_bind     3695..3714
                     /dnas_title="pTC14_F1 primer"
                     /vntifkey="28"
                     /label=pTC14_F1 primer
     misc_feature    1633..1633
                     /dnas_title="end"
                     /vntifkey="21"
                     /label=end
     primer_bind     1703..1724
                     /dnas_title="pTC14_R1 primer"
                     /vntifkey="28"
                     /label=pTC14_R1 primer
     misc_feature    1..1
                     /dnas_title="start"
                     /vntifkey="21"
                     /label=start
     primer_bind     1607..1626
                     /dnas_title="pTC14_F2 primer"
                     /vntifkey="28"
                     /label=pTC14_F2 primer
     misc_feature    2626..3299
                     /dnas_title="ori"
                     /vntifkey="21"
                     /label=ori
     misc_feature    1754..1759
                     /note="BsaI site"
     misc_feature    2556..2561
                     /note="BsaI site"
     misc_feature    1761..1764
                     /note="4nt"
     misc_feature    1765..2554
                     /note="4nt"
     terminator      1765..2068
                     /note="AtU6 promoter"
     promoter        2185..2550
                     /note="terminator"
     misc_recomb     2070..2101
                     /note="Guid RNA cloning site"
     stem_loop       2102..2184
                     /note="guide RNA scaffold"
     -35_signal      2004..2014
                     /note="-35 signal"
     TATA_signal     2038..2045
                     /note="TATA"
     PCR_product     1765..2550
                     /pair="Pair 1"
                     /set="Set 1"
                     /current=1
                     /score="0.000"
     PCR_frame       1765..2550
                     /note="Reading Frame"
                     /set="Set 1"
     source          1..3803
                     /dnas_title="pHD4"
ORIGIN      
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcctct agaggtctcg ccagAGAAAT CTCAAAATTC CGGCAGAACA ATTTTGAATC
     1801 TCGATCCGTA GAAACcAGAC GGTCATTGTT TTAGTTCCAC CACGATTATA TTTGAAATTT
     1861 ACGTGAGTGT GAGTGAGACT TGCATAAGAA AATAAAATCT TTAGTTGGGA AAAAATTCAA
     1921 TAATATAAAT GGGCTTGAGA AGGAAGCGAG GGATAGGCCT TTTTCTAAAA TAGGCCCATT
     1981 TAAGCTATTA ACAATCTTCA AAAGTACCAC AGCGCTTAGG TAAAGAAAGC AGCTGAGTTT
     2041 ATATATGGTT AGAcACGAAG TAGTGATTGg agacgagatc tagtctgagt cgaccgtctc
     2101 tGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG
     2161 TGGCACCGAG TCGGTGCTTT TTTTGGCAAA AATTTTCAGA TTTTTTCTTC ATCTGTAGAT
     2221 TTCTGGGTTT TTTTTTCCGT TTCGTGAATC ATAAGTGAAG TTTTGGATGC AAATCTGCGC
     2281 GAAAAAAGTT GGACCTGCAA TGAGCTTATT TAGATAGCTA AGACAAAGTG ATTGGTCCGT
     2341 TGTTTCAGTT CTGATTGTCA GAGAGTTTGT TTCGAGtCGG CGACACCAAT GCGTTTTGTT
     2401 AACCAGATTT CGGGTAAGAA ATGTATCGAG AGTTTGTTTC cAGACGGCTA CATCATTTTC
     2461 TTATGAAGGG TGAAATTAGA TAGACCAAAG ATTGAAACAC AACATTTCTT TCACAAAAAT
     2521 ATAATAAACT TGATAGCATT TAGGATCAGC tgttggagac cctcgagcca cccatgacca
     2581 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3421 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3481 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3541 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3601 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3661 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3721 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3781 tggcagttcc ctactctcgc gtt
//

