LOCUS       pYPQ104_correct_        3623 bp ds-DNA     circular     02-MAR-2014
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     3154..3171
                     /dnas_title="M13-fwd"
                     /vntifkey="28"
                     /label=M13-fwd
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(633..653)
                     /dnas_title="M13-rev"
                     /vntifkey="28"
                     /label=M13-rev
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(613..634)
                     /dnas_title="T7"
                     /vntifkey="28"
                     /label=T7
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1942..2615
                     /dnas_title="pUC origin"
                     /vntifkey="21"
                     /label=pUC origin
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    865..1875
                     /dnas_title="SpnR"
                     /vntifkey="21"
                     /label=SpnR
                     /ApEinfo_fwdcolor="#f70938"
                     /ApEinfo_revcolor="#f70938"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    731..864
                     /dnas_title="Spn promoter"
                     /vntifkey="21"
                     /label=Spn promoter
                     /ApEinfo_fwdcolor="#2fec13"
                     /ApEinfo_revcolor="#2fec13"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3155..3170
                     /dnas_title="M13-F"
                     /vntifkey="21"
                     /label=M13-F
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(637..653)
                     /dnas_title="M13-R"
                     /vntifkey="21"
                     /label=M13-R
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    497..596
                     /dnas_title="attL2"
                     /vntifkey="21"
                     /label=attL2
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     insertion_seq   1..13
                     /dnas_title="For Left homology arm"
                     /vntifkey="14"
                     /label=For Left homology arm
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3173..3285
                     /dnas_title="attL5"
                     /vntifkey="21"
                     /label=attL5
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1..3298
                     /dnas_title="pYPQ141C"
     PCR_primer      3138..3161
                     /note="M13-F1"
     terminator      409..489
                     /note="OsU6-2 terminator"
     terminator      333..415
                     /note="gRNA scaffold"
     misc_structure  301..332
                     /note="guide RNA cloning site"
     TATA_signal     273..276
                     /note="TATA box"
     PCR_primer      complement(467..489)
                     /primer="AAGCTTATCGATACCCTCGACCT"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
     PCR_primer      complement(467..489)
                     /note="pYPQ13xC-R"
     PCR_product     19..489
                     /pair="Pair 1"
                     /set="Set 1"
                     /current=1
                     /score="0.000"
     PCR_primer      19..23
                     /primer="ACTAG"
                     /set="Set 1"
                     /pair="Pair 1"
                     /current=1
                     /original="GGTACCGAGCTCGGATCCACTAG"
     promoter        19..299
                     /note="OsU6-2 promoter"
     PCR_frame       19..489
                     /note="Reading Frame"
                     /set="Set 1"
ORIGIN      
        1 ctagataacg caggatccAC TAGTAACGGC CGCCAGTGTG CTGGAATTGC CCTTGGATCA
       61 TGAACCAACG GCCTGGCTGT ATTTGGTGGT TGTGTAGGGA GATGGGGAGA AGAAAAGCCC
      121 GATTCTCTTC GCTGTGATGG GCTGGATGCA TGCGGGGGAG CGGGAGGCCC AAGTACGTGC
      181 ACGGTGAGCG GCCCACAGGG CGAGTGTGAG CGCGAGAGGC GGGAGGAACA GTTTAGTACC
      241 ACATTGCCCA GCTAACTCGA ACGCGACCAA CTTATAAACC CGCGCGCTGT CGCTTGTGTG
      301 gagacgagat ctagtctgag tcgaccgtct ctGTTTTAGA GCTAGAAATA GCAAGTTAAA
      361 ATAAGGCTAG TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTTGTCCC
      421 TTCGAAGGGC AATTCTGCAG ATATCCATCA CACTGGCGGC CGCTCGAGGT CGAgGGTATC
      481 GATAAGCTTg aattcgaccc agctttcttg tacaaagttg gcattataaa aaataattgc
      541 tcatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
      601 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
      661 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc
      721 ctcctctgga ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg
      781 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
      841 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      901 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
      961 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
     1021 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     1081 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1141 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1201 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1261 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     1321 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     1381 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     1441 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     1501 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     1561 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     1621 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     1681 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     1741 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     1801 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
     1861 gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
     1921 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1981 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2041 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2101 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2161 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2221 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2281 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2341 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2401 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2461 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2521 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2581 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2641 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2701 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2761 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     2821 gcgggcagtg agcgcaacgc aattaatacg cgtaccgctc gccaggaaga gtttgtagaa
     2881 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     2941 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3001 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3061 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3121 acgcttgcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3181 gggcccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
     3241 agcaatgctt ttttataatg ccaactttgt atacaaaagt tgccccatgg cgttccct
//

