LOCUS       pYPQ104_correct_        3623 bp ds-DNA     circular     02-MAR-2014
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     3169..3186
                     /dnas_title="M13-fwd"
                     /vntifkey="28"
                     /label=M13-fwd
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(648..668)
                     /dnas_title="M13-rev"
                     /vntifkey="28"
                     /label=M13-rev
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(628..649)
                     /dnas_title="T7"
                     /vntifkey="28"
                     /label=T7
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1957..2630
                     /dnas_title="pUC origin"
                     /vntifkey="21"
                     /label=pUC origin
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    880..1890
                     /dnas_title="SpnR"
                     /vntifkey="21"
                     /label=SpnR
                     /ApEinfo_fwdcolor="#f70938"
                     /ApEinfo_revcolor="#f70938"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    746..879
                     /dnas_title="Spn promoter"
                     /vntifkey="21"
                     /label=Spn promoter
                     /ApEinfo_fwdcolor="#2fec13"
                     /ApEinfo_revcolor="#2fec13"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3170..3185
                     /dnas_title="M13-F"
                     /vntifkey="21"
                     /label=M13-F
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(652..668)
                     /dnas_title="M13-R"
                     /vntifkey="21"
                     /label=M13-R
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    512..611
                     /dnas_title="attL2"
                     /vntifkey="21"
                     /label=attL2
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3188..3300
                     /dnas_title="attL5"
                     /vntifkey="21"
                     /label=attL5
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1..3313
                     /dnas_title="pYPQ145"
     misc_feature    34..34
                     /label=CUT 1
                     /note="CUT 1"
     misc_feature    complement(62..337)
                     /label=lacZ'
                     /note="lacZ'"
     promoter        complement(338..459)
                     /label=lacZ promoter
                     /note="lacZ promoter"
     misc_feature    19..46
                     /label=FUS_A_left
                     /note="FUS_A_left"
     misc_feature    476..488
                     /label=FUS_A_right
                     /note="FUS_A_right"
     modified_base   505..505
                     /note="G to A mutation"
ORIGIN      
        1 ctagataacg caggatcccc aagtggtggc tatcgagacc ggcgccgcta cagggcgcgt
       61 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      121 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      181 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
      241 tatagggcga attgggtacc gggccccccc tcgaggtcct ccagcttttg ttccctttag
      301 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccaccgg
      481 tggtctctTG CAactagtaa gggcgaattc gacccagctt tcttgtacaa agttggcatt
      541 ataaaaaata attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      601 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
      661 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
      721 aatatatcat catgcctcct ctggaccagc caggacagaa atgcctcgac ttcgctgctg
      781 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
      841 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
      901 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
      961 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     1021 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     1081 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     1141 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     1201 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     1261 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     1321 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     1381 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     1441 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     1501 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     1561 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     1621 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1681 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1741 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     1801 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     1861 ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt
     1921 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1981 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2041 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2101 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2161 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2221 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2281 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2341 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     2401 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2461 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2521 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2581 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2641 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2701 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2761 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     2821 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctcgccag
     2881 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     2941 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3001 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3061 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3121 cctactctcg cgttaacgct tgcatggatg ttttcccagt cacgacgttg taaaacgacg
     3181 gccagtctta agctcgggcc caaataatga ttttattttg actgatagtg acctgttcgt
     3241 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtataca aaagttgccc
     3301 catggcgttc cct
//

