LOCUS       pDonor_mU6n             4027 bp    DNA     circular     20-AUG-2015
FEATURES             Location/Qualifiers
     terminator      421..426
                     /note="U6 terminator"
     misc_feature    337..342
                     /note="BbsI 5'"
     misc_feature    345..420
                     /note="chimeric guide RNA scaffold 1"
     misc_feature    337..844
                     /note="Donor fragment"
     misc_feature    511..832
                     /dnas_title="mU6"
                     /vntifkey="21"
                     /label=mU6
     misc_feature    602..609
                     /note="Octamer motif"
     misc_feature    complement(839..844)
                     /note="BbsI 3'"
     misc_feature    764..783
                     /note="proximal sequence element"
     misc_feature    801..809
                     /note="TATA-Box"
     source          1..4027
                     /dnas_title="pDonor_mU6n"
ORIGIN      
        1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
       61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
      121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
      181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTAT
      241 TTAGGTGACA CTATAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA
      301 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTGAAG ACCTGTTTTA GAGCTAGAAA
      361 TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC
      421 TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT TTTAGCGCGT
      481 GCGCCAATTC TGCAGACAAA TGGCTCTAGA TCTAGAGATC CGACGCCGCC ATCTCTAGGC
      541 CCGCGCCGGC CCCCTCGCAC AGACTTGTGG GAGAAGCTCG GCTACTCCCC TGCCCCGGTT
      601 AATTTGCATA TAATATTTCC TAGTAACTAT AGAGGCTTAA TGTGCGATAA AAGACAGATA
      661 ATCTGTTCTT TTTAATACTA GCTACATTTT ACATGATAGG CTTGGATTTC TATAAGAGAT
      721 ACAAATACTA AATTATTATT TTAAAAAACA GCACAAAAGG AAACTCACCC TAACTGTAAA
      781 GTAATTGTGT GTTTTGAGAC TATAAATATG CATGCGAGAA AAGCCTTGTT TGGTTTGGGT
      841 CTTCAAGGGC GAATTCTGCA GATATCCATC ACACTGGCGG CCGCTCGAGC ATGCATCTAG
      901 AGGGCCCAAT TCGCCCTATA GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG
      961 TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT
     1021 CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG
     1081 CCTATACGTA CGGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT
     1141 TGTGGATGTA CAGAGTGATA TTATTGACAC GCCGGGGCGA CGGATGGTGA TCCCCCTGGC
     1201 CAGTGCACGT CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG
     1261 GGATGAAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG
     1321 GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT
     1381 GTTCTGGGGA ATATAAATGT CAGGCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     1441 TTTCACGTAG AAAGCCAGTC CGCAGAAACG GTGCTGACCC CGGATGAATG TCAGCTACTG
     1501 GGCTATCTGG ACAAGGGAAA ACGCAAGCGC AAAGAGAAAG CAGGTAGCTT GCAGTGGGCT
     1561 TACATGGCGA TAGCTAGACT GGGCGGTTTT ATGGACAGCA AGCGAACCGG AATTGCCAGC
     1621 TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAAGTA AACTGGATGG CTTTCTCGCC
     1681 GCCAAGGATC TGATGGCGCA GGGGATCAAG CTCTGATCAA GAGACAGGAT GAGGATCGTT
     1741 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
     1801 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
     1861 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
     1921 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
     1981 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
     2041 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
     2101 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
     2161 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
     2221 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
     2281 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
     2341 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
     2401 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
     2461 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
     2521 TCTTGACGAG TTCTTCTGAA TTATTAACGC TTACAATTTC CTGATGCGGT ATTTTCTCCT
     2581 TACGCATCTG TGCGGTATTT CACACCGCAT ACAGGTGGCA CTTTTCGGGG AAATGTGCGC
     2641 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA
     2701 TAACCCTGAT AAATGCTTCA ATAATAGCAC GTGAGGAGGG CCACCATGGC CAAGTTGACC
     2761 AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT CTGGACCGAC
     2821 CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT CCGGGACGAC
     2881 GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC CCTGGCCTGG
     2941 GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT GTCCACGAAC
     3001 TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG GGGGCGGGAG
     3061 TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA GCAGGACTGA
     3121 CACGTGCTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT
     3181 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA
     3241 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA
     3301 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT
     3361 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG
     3421 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
     3481 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA
     3541 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC
     3601 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC
     3661 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA
     3721 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG
     3781 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA
     3841 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG GCTTTTGCTG GCCTTTTGCT
     3901 CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG
     3961 TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA
     4021 GCGGAAG
//

