LOCUS       pDonor_sU6              3964 bp    DNA     circular     20-AUG-2015
FEATURES             Location/Qualifiers
     terminator      421..426
                     /note="U6 terminator"
     misc_feature    337..342
                     /note="BbsI 5'"
     misc_feature    345..420
                     /note="chimeric guide RNA scaffold 1"
     misc_feature    337..781
                     /note="Donor fragment"
     misc_feature    539..546
                     /note="Octamer Motif"
     promoter        join(511..511,511..544)
                     /dnas_title="hU6"
                     /label=hU6
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     misc_feature    547..700
                     /note="mU6"
     misc_feature    511..769
                     /note="hmU6_promoter hybrid"
     misc_feature    complement(776..781)
                     /note="BbsI 3'"
     misc_feature    701..720
                     /note="Proximal sequence element"
     misc_feature    701..720
                     /note=""
     misc_feature    721..737
                     /note=""
     misc_feature    738..746
                     /note="TATA-Box"
     misc_feature    738..746
                     /note=""
     misc_feature    747..769
                     /dnas_title="mU6"
                     /vntifkey="21"
                     /label=mU6
     source          1..3964
                     /dnas_title="pDonor_sU6"
ORIGIN      
        1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
       61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
      121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
      181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTAT
      241 TTAGGTGACA CTATAGAATA CTCAAGCTAT GCATCAAGCT TGGTACCGAG CTCGGATCCA
      301 CTAGTAACGG CCGCCAGTGT GCTGGAATTC GCCCTTGAAG ACCTGTTTTA GAGCTAGAAA
      361 TAGCAAGTTA AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC
      421 TTTTTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT TTTAGCGCGT
      481 GCGCCAATTC TGCAGACAAA TGGCTCTAGA GAGGGCCTAT TTCCCATGAT TCCTTCATAT
      541 TTGCATATAA TATTTCCTAG TAACTATAGA GGCTTAATGT GCGATAAAAG ACAGATAATC
      601 TGTTCTTTTT AATACTAGCT ACATTTTACA TGATAGGCTT GGATTTCTAT AAGAGATACA
      661 AATACTAAAT TATTATTTTA AAAAACAGCA CAAAAGGAAA CTTACCGTAA CTTGAAAGTA
      721 ATTGTGTGTT TTGAGACTTT ATATATGCAT GCGAGAAAAG CCTTGTTTGG TTTGGGTCTT
      781 CAAGGGCGAA TTCTGCAGAT ATCCATCACA CTGGCGGCCG CTCGAGCATG CATCTAGAGG
      841 GCCCAATTCG CCCTATAGTG AGTCGTATTA CAATTCACTG GCCGTCGTTT TACAACGTCG
      901 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC
      961 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT
     1021 ATACGTACGG CAGTTTAAGG TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT
     1081 GGATGTACAG AGTGATATTA TTGACACGCC GGGGCGACGG ATGGTGATCC CCCTGGCCAG
     1141 TGCACGTCTG CTGTCAGATA AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA
     1201 TGAAAGCTGG CGCATGATGA CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA
     1261 AGAAGTGGCT GATCTCAGCC ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT
     1321 CTGGGGAATA TAAATGTCAG GCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1381 CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG ATGAATGTCA GCTACTGGGC
     1441 TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG GTAGCTTGCA GTGGGCTTAC
     1501 ATGGCGATAG CTAGACTGGG CGGTTTTATG GACAGCAAGC GAACCGGAAT TGCCAGCTGG
     1561 GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT TCTCGCCGCC
     1621 AAGGATCTGA TGGCGCAGGG GATCAAGCTC TGATCAAGAG ACAGGATGAG GATCGTTTCG
     1681 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
     1741 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
     1801 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
     1861 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
     1921 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
     1981 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
     2041 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
     2101 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
     2161 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
     2221 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
     2281 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA
     2341 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
     2401 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT
     2461 TGACGAGTTC TTCTGAATTA TTAACGCTTA CAATTTCCTG ATGCGGTATT TTCTCCTTAC
     2521 GCATCTGTGC GGTATTTCAC ACCGCATACA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA
     2581 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA
     2641 CCCTGATAAA TGCTTCAATA ATAGCACGTG AGGAGGGCCA CCATGGCCAA GTTGACCAGT
     2701 GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG GACCGACCGG
     2761 CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG GGACGACGTG
     2821 ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT GGCCTGGGTG
     2881 TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC CACGAACTTC
     2941 CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG GCGGGAGTTC
     3001 GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA GGACTGACAC
     3061 GTGCTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC
     3121 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     3181 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     3241 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     3301 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
     3361 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     3421 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     3481 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     3541 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
     3601 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     3661 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     3721 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     3781 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGGCT TTTGCTGGCC TTTTGCTCAC
     3841 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA
     3901 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG
     3961 GAAG
//

