LOCUS pTKW106_alpA 10768 bp DNA circular 14-MAR-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|656503190| COMMENT VNTDBDATE|656506798| COMMENT LSOWNER| COMMENT VNTNAME|pTKW106_alpA| COMMENT VNTAUTHORNAME|Arthur Prindle| COMMENT VNTAUTHOREML|abprindle@gmail.com| FEATURES Location/Qualifiers misc_feature complement(7694..10768) /vntifkey="21" /label=lacZ terminator complement(7588..7693) /vntifkey="43" /label=T1 misc_feature complement(2941..4023) /vntifkey="21" /label=lacI promoter complement(4024..4080) /vntifkey="30" /label=pLacIq rep_origin complement(316..1099) /vntifkey="33" /label=colE1 promoter complement(1..74) /vntifkey="30" /label=pTac misc_feature 1100..1894 /vntifkey="21" /label=KanR misc_feature 4087..7587 /vntifkey="21" /label=alp7A\HS\frag misc_feature 1895..2474 /vntifkey="21" /label=hok BASE COUNT 2848 a 2651 c 2612 g 2657 t ORIGIN 1 gaatactgtt tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg 61 atgattaatt gtcatatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga 121 tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg 181 tgcaccgtgc agtcgatgat aagctgtcaa acatgagaat taattctgca ttaatgaatc 241 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 301 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 361 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 421 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 481 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 541 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 601 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 661 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 721 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 781 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 841 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 901 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 961 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 1021 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 1081 gacgctcagt ggaacgaaat cagaagaact cgtcaagaag gcgatagaag gcgatgcgct 1141 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa 1201 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca 1261 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc 1321 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg 1381 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa 1441 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg 1501 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt 1561 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca 1621 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg 1681 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt 1741 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag 1801 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg 1861 gagaacctgc gtgcaatcca tcttgttcaa tcataacaaa ctccgggagg cagcgtgatg 1921 cggcaacaat cacacggatt tcccgtgaac ggtctgaatg agcggattat tttcagggaa 1981 agtgagtgtg gtcagcgtgc aggtatatgg gctatgatgt gcccggcgct tgaggctttc 2041 tgcctcatga cgtgaaggtg gtttgttgcc gtgttgtgtg gcagaaagaa gatagccccg 2101 tagtaagtta attttcatta accaccacga ggcatcccta tgtctagtcc acatcaggat 2161 agcctcttac cgcgctttgc gcaaggagaa gaaggccatg aaactaccac gaagttccct 2221 tgtctggtgt gtgttgatcg tgtgtctcac actgttgata ttcacttatc tgacacgaaa 2281 atcgctgtgc gagattcgtt acagagacgg acacagggag gtggcggctt tcatggctta 2341 cgaatccggt aagtagcaac ctggaggcgg gcgcaggccc gccttttcag gactgatgct 2401 ggtctgacta ctgaagcgcc tttataaagg ggctgctggt tcgccggtag cccctttctc 2461 cttgctgatg ttgtgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 2521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 2581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 2641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 2701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 2761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 2821 gttccgcgca catttccccg aaaagtgcca cctgacgtga ggttcttgca ggtcggaagc 2881 ataaagtgta aagcctgggg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc 2941 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 3001 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga 3061 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc 3121 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata 3181 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag 3241 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat 3301 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc 3361 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg 3421 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat 3481 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga 3541 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt 3601 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag 3661 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct 3721 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc 3781 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa 3841 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat 3901 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg 3961 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt 4021 catcctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca 4081 cctaggtcat tagcctccaa tcttatagtg aaactccgca aacttcgttt cctcaatatt 4141 gggaatactc gataactttt gttcagcttt ttcataaaaa gaaattgctt taacataatt 4201 ttttttaaaa aactcataat cccctgcaaa caattcaaaa taaaaattta ttaaaagatc 4261 atgacttccc ttacttaaca tattaacgac atcacccata gatgcctctt gatcaaaaca 4321 aaggctatga cgatgatcca gtagaataaa ataatcaacc aggtccttat tatcttcagc 4381 ctgacttaac aattgcttta tttcagttct tttctctata gactcatgaa ccttctcctg 4441 cataatatag tcatgccatt tattcaacag attgcctaca tagggagacg gtactttttt 4501 tactttggac aacatacact tccctccagc tattcaaaaa tcaaaactag tcctaatact 4561 tatcggcatc atcaaacatt ttaaaacaca gaacatttag tacatagtgc ttaatgttca 4621 aatctcattt tgatgctctg aacccaagca tgtactgata tcatactaaa cggctcaaac 4681 acaatctata caagttttta agataggcca aagggaataa cagtatacgt tagtgaaatc 4741 ccacaggaaa aatatattaa aatactaatg ttctatcaaa cgaacaaccc ttaaaaagga 4801 acttaaaacc tctgggtttt aaggaaattc gcattttatt tagtgttttt ctcttgactt 4861 tgagaacttg aaactagcag aatagctgac tgttctagga aacagggcga atttcgattg 4921 cctatgtctg tcgcgcaaaa aataaaaacg gacagacata ggcaatcgat caggatttga 4981 aactagcgtc atagagacgt ctgaggtttc cagctctgcc ttgctatcgc caggctttcg 5041 cctgccatga cctttttaca tacaatgctt gtcctgtatg caacttctat ggggtttgtc 5101 tcgtgttctc tcacacggtc acactcaatt gtgtgccgct gcatagaagc ttggccatag 5161 ttgcccgcac cgtagtgcgc caagcaacct agtggtttat ccacattctc cggaccgtta 5221 atggccgtcc tcgccattca ccacaagcgc agcaaggaac gcttattgtg gtatatcccc 5281 gggtttgcgg tggacggggc aactcctgac gtcagtttat tttacacccc ttaacggcag 5341 ctgggtgaca aacaaaaaac gacagaaaac cacggtttga taccctccaa acagtggttt 5401 tctgtcgtcc aaaaatagcc gaaaagtgtt gacgtataca cttgttttcg gtaaaatgaa 5461 gacataactt aaacattgta agtgagggct tacaaaccaa gtgttcgatg ctgcaacatc 5521 ggacactttt tatttgtcat tctttatttg tattcaattt tgcaaatagc tcgcaaacaa 5581 aatatgtatc atcaaatcta ttaaccttgt tgtctgcaaa caacagggtt ttttgttgtt 5641 tatttagaat aactagaacc agaattcaat gccaaaactt tcacattgac ttaacttgac 5701 tttatcttac acgatttttt ttttgacgta aagccccggg cctgaaatca cttttctcta 5761 ctgatttcac tgatttcatt tttattatat aatcctcaaa tagcctgtat tcactgattt 5821 taaatgtgat ttcattttat tgactttagt gatataagat gctagtattg aggaaagtga 5881 aatcaaagga gagaataaaa atatgaatat ttctcgtatg aacgtggact ttggaaacag 5941 tatgtacatg aatttaattg atggttattt ttttgaattg cctacaaatg tagtagagat 6001 atctaaagaa gctgctgaag gaaaatttac gagtatcgtt gaagatccgg cagatttaaa 6061 ggaccggtta ttagtttcta cagttattga tgaaacagag agatattttc tagttggtga 6121 acttgctgaa ccagaagtgt taggcaacca acacatcaag aagttacata ataaagtaga 6181 gtcacatatt ccatacgtaa catttttagc tgcaactgct tattaccaag cgctaaaagg 6241 caaacgtgaa gataatgaag ttactattga atactttcaa acaatgctac caatttggct 6301 tcttaaaaaa ttggataagt tcagtgaaat gcagaaaagg atggcatcta aatttttggg 6361 cactcaccaa gtaaaggtgc tgacattagg attagaaaaa gagcttacta taaaagtgga 6421 agatgcagcg tgcaggatcg aatctgaagt agcaagatgg gcaataaaga aaaactttga 6481 cctagaagat aaagactatg ccgaacaatt taaaaattat gacgtagttt tttgtgattt 6541 aggtggcgga acagatgatc tagtattact accagctgga ttaaaaccgc caaaaagtcg 6601 tgattctttt gtttctaata ccgaagcacc gtttttagcg cacttagaaa aattgagaaa 6661 agaaaaactc ctagagcact ttgatagcgt tagggagctt gaaaagttta tatactcaaa 6721 tattggaaaa actaagatgg aacgaagaga cgggaatacc ggtcagaaat ttgatttaac 6781 tgatatcatc aaaaaatctc ttaaagaata cacagaaatc aaaatagccc aagctgaaaa 6841 tacgttccct gcaccaaaag ataaggttta caaatacctt tattttggcg gtgttggcga 6901 ggtgcttgaa gaatcaatta gtgtggttac tgaagagaga tatggccgtg atatttctga 6961 atcaaatcat atagttgctg aggatgcaag actgctcaac ttatatggcc ttgaagtttt 7021 aagccgcgct gaacaagtaa agaaacaggc aaatgaaaaa gaggcacaat caatttaggt 7081 gattagaaat ggggaaaaac aaaagaattc cactctttaa tgtccgaaca acacaaatgt 7141 ctgatgaaat gtacgatttt gttttagagc agattagtac attcagtaaa ggtaagagta 7201 agggtacctt tagagagtat gcctttcagc tcatagaaag ggacatgcaa caacagaaag 7261 aggaacagca aaatagagaa aaagatcgtc atgttcatga tgaattaatt gccatgagag 7321 aagaaatgaa aaaagaattt cgtgatttga ggaaaaaaat tgatcaggga tcgatctacg 7381 tagaacacaa aacagctgat ccaaagtcag cttcaaaaac gattgaagaa ggtcagttaa 7441 tcactgaaaa aatcactgga actattgaag aagaatacga ctatgatttt taagagcctg 7501 gattaatcta ggctcttttt ttatgccatt taagggagga ttgcatgaca aacttttttt 7561 agttgcaaca cagacgccct gagcaaccgg cggatttgtc ctactcagga gagcgttcac 7621 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg 7681 atgcctctag cacttagact cgagcggccg ctttttgaca ccagaccaac tggtaatggt 7741 agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag tcgtcgccac 7801 caatccccat atggaaaccg tcgatattca gccatgtgcc ttcttccgcg tgcagcagat 7861 ggcgatggct ggtttccatc agttgctgtt gactgtagcg gctgatgttg aactggaagt 7921 cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc agaccgtttt 7981 cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag cggtcaaaac 8041 aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc cagtttaccc 8101 gctctgctac ctgcgccagc tggcagttca ggccaatccg cgccggatgc ggtgtatcgc 8161 tcgccacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc cggtaggttt 8221 tccggctgat aaataaggtt ttcccctgat gctgccacgc gtgagcggtc gtaatcagca 8281 ccgcatcagc aagtgtatct gccgtgcact gcaacaacgc tgcttcggcc tggtaatggc 8341 ccgccgcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct tcacttacgc 8401 caatgtcgtt atccagcggt gcacgggtga actgatcgcg cagcggcgtc agcagttggt 8461 ttttatcgcc aatccacatc tgtgaaagag agcctgactg gcggttaaat tgccaacgct 8521 tattacccag ctcgatgcaa aaatccattt cgctggtggt cagatgcggg atggcgtggg 8581 acgcggcggg gagcgtcaca ctgaggtttt ccgccagacg ccactgctgc caggcgctga 8641 tgtgcccggc ttctgaccat gcggtcgcgt tcggttgcac tacgcgtact gtgagccaga 8701 gttgcccggc gctctccggc tgcggtagtt caggcagttc aatcaactgt ttaccttgtg 8761 gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc gccaccatcc 8821 agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact tcgatggttt 8881 gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc agcgctggat 8941 gcggcgtgcg gtcggcaaag accagaccgt tcatacagaa ctggcgatcg ttcggcgtat 9001 cgccaaaatc accgccgtaa gccgaccacg ggttgccgtt ttcatcatat ttaatcagcg 9061 actgatccac ccagtcccag acgaagccgc cctgtaaacg gggatactga cgaaacgcct 9121 gccagtattt agcgaaaccg ccaagactgt tacccatcgc gtgggcgtat tcgcaaagga 9181 tcagcgggcg cgtctctcca ggtagcgaaa gccatttttt gatggaccat ttcggcacag 9241 ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata tcggtggccg 9301 tggtgtcggc tccgccgcct tcatactgca ccgggcggga aggatcgaca gatttgatcc 9361 agcgatacag cgcgtcgtga ttagcgccgt ggcctgattc attccccagc gaccagatga 9421 tcacactcgg gtgattacga tcgcgctgca ccattcgcgt tacgcgttcg ctcatcgccg 9481 gtagccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg gtttcaatat 9541 tggcttcatc caccacatac aggccgtagc ggtcgcacag cgtgtaccac agcggatggt 9601 tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcatcagc aggatatcct 9661 gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg tgacggttaa 9721 cgcctcgaat cagcaacggc ttgccgttca gcagcagcag accattttca atccgcacct 9781 cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt 9841 caaccaccgc acgatagaga ttcgggattt cggcgctcca cagtttcggg ttttcgacgt 9901 tcagacgtag tgtgacgcga tcggcataac caccacgctc atcgataatt tcaccgccga 9961 aaggcgcggt gccgctggcg acctgcgttt caccctgcca taaagaaact gttacccgta 10021 ggtagtcacg caactcgccg cacatctgaa cttcagcctc cagtacagcg cggctgaaat 10081 catcattaaa gcgagtggca acatggaaat cgctgatttg tgtagtcggt ttatgcagca 10141 acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc agataactgc 10201 cgtcactcca gcgcagcacc atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc 10261 tcaggtcaaa ttcagacggc aaacgactgt cctggccgta accgacccag cgcccgttgc 10321 accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg ccttcctgta 10381 gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa 10441 caaacggcgg attgaccgta atgggatagg tcacgttggt gtagatgggc gcatcgtaac 10501 cgtgcatctg ccagtttgag gggacgacga cagtatcggc ctcaggaaga tcgcactcca 10561 gccagctttc cggcaccgct tctggtgccg gaaaccaggc aaagcgccat tcgccattca 10621 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 10681 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 10741 gacgttgtaa aacgacggga tctatcat //