LOCUS       pTKW106_alpA           10768 bp    DNA     circular     14-MAR-2013
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|656503190|
COMMENT     VNTDBDATE|656506798|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTKW106_alpA|
COMMENT     VNTAUTHORNAME|Arthur Prindle|
COMMENT     VNTAUTHOREML|abprindle@gmail.com|
FEATURES             Location/Qualifiers
     misc_feature    complement(7694..10768)
                     /vntifkey="21"
                     /label=lacZ
     terminator      complement(7588..7693)
                     /vntifkey="43"
                     /label=T1
     misc_feature    complement(2941..4023)
                     /vntifkey="21"
                     /label=lacI
     promoter        complement(4024..4080)
                     /vntifkey="30"
                     /label=pLacIq
     rep_origin      complement(316..1099)
                     /vntifkey="33"
                     /label=colE1
     promoter        complement(1..74)
                     /vntifkey="30"
                     /label=pTac
     misc_feature    1100..1894
                     /vntifkey="21"
                     /label=KanR
     misc_feature    4087..7587
                     /vntifkey="21"
                     /label=alp7A\HS\frag
     misc_feature    1895..2474
                     /vntifkey="21"
                     /label=hok
BASE COUNT     2848 a      2651 c      2612 g      2657 t 
ORIGIN
        1 gaatactgtt tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg 
       61 atgattaatt gtcatatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga 
      121 tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg 
      181 tgcaccgtgc agtcgatgat aagctgtcaa acatgagaat taattctgca ttaatgaatc 
      241 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
      301 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
      361 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
      421 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
      481 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
      541 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
      601 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 
      661 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
      721 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
      781 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
      841 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
      901 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
      961 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     1021 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     1081 gacgctcagt ggaacgaaat cagaagaact cgtcaagaag gcgatagaag gcgatgcgct 
     1141 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa 
     1201 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca 
     1261 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc 
     1321 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg 
     1381 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa 
     1441 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg 
     1501 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt 
     1561 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca 
     1621 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg 
     1681 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt 
     1741 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag 
     1801 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg 
     1861 gagaacctgc gtgcaatcca tcttgttcaa tcataacaaa ctccgggagg cagcgtgatg 
     1921 cggcaacaat cacacggatt tcccgtgaac ggtctgaatg agcggattat tttcagggaa 
     1981 agtgagtgtg gtcagcgtgc aggtatatgg gctatgatgt gcccggcgct tgaggctttc 
     2041 tgcctcatga cgtgaaggtg gtttgttgcc gtgttgtgtg gcagaaagaa gatagccccg 
     2101 tagtaagtta attttcatta accaccacga ggcatcccta tgtctagtcc acatcaggat 
     2161 agcctcttac cgcgctttgc gcaaggagaa gaaggccatg aaactaccac gaagttccct 
     2221 tgtctggtgt gtgttgatcg tgtgtctcac actgttgata ttcacttatc tgacacgaaa 
     2281 atcgctgtgc gagattcgtt acagagacgg acacagggag gtggcggctt tcatggctta 
     2341 cgaatccggt aagtagcaac ctggaggcgg gcgcaggccc gccttttcag gactgatgct 
     2401 ggtctgacta ctgaagcgcc tttataaagg ggctgctggt tcgccggtag cccctttctc 
     2461 cttgctgatg ttgtgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     2521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     2581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     2641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     2701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     2761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     2821 gttccgcgca catttccccg aaaagtgcca cctgacgtga ggttcttgca ggtcggaagc 
     2881 ataaagtgta aagcctgggg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc 
     2941 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
     3001 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga 
     3061 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc 
     3121 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata 
     3181 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag 
     3241 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat 
     3301 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc 
     3361 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg 
     3421 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat 
     3481 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga 
     3541 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt 
     3601 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag 
     3661 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct 
     3721 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc 
     3781 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa 
     3841 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat 
     3901 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg 
     3961 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt 
     4021 catcctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca 
     4081 cctaggtcat tagcctccaa tcttatagtg aaactccgca aacttcgttt cctcaatatt 
     4141 gggaatactc gataactttt gttcagcttt ttcataaaaa gaaattgctt taacataatt 
     4201 ttttttaaaa aactcataat cccctgcaaa caattcaaaa taaaaattta ttaaaagatc 
     4261 atgacttccc ttacttaaca tattaacgac atcacccata gatgcctctt gatcaaaaca 
     4321 aaggctatga cgatgatcca gtagaataaa ataatcaacc aggtccttat tatcttcagc 
     4381 ctgacttaac aattgcttta tttcagttct tttctctata gactcatgaa ccttctcctg 
     4441 cataatatag tcatgccatt tattcaacag attgcctaca tagggagacg gtactttttt 
     4501 tactttggac aacatacact tccctccagc tattcaaaaa tcaaaactag tcctaatact 
     4561 tatcggcatc atcaaacatt ttaaaacaca gaacatttag tacatagtgc ttaatgttca 
     4621 aatctcattt tgatgctctg aacccaagca tgtactgata tcatactaaa cggctcaaac 
     4681 acaatctata caagttttta agataggcca aagggaataa cagtatacgt tagtgaaatc 
     4741 ccacaggaaa aatatattaa aatactaatg ttctatcaaa cgaacaaccc ttaaaaagga 
     4801 acttaaaacc tctgggtttt aaggaaattc gcattttatt tagtgttttt ctcttgactt 
     4861 tgagaacttg aaactagcag aatagctgac tgttctagga aacagggcga atttcgattg 
     4921 cctatgtctg tcgcgcaaaa aataaaaacg gacagacata ggcaatcgat caggatttga 
     4981 aactagcgtc atagagacgt ctgaggtttc cagctctgcc ttgctatcgc caggctttcg 
     5041 cctgccatga cctttttaca tacaatgctt gtcctgtatg caacttctat ggggtttgtc 
     5101 tcgtgttctc tcacacggtc acactcaatt gtgtgccgct gcatagaagc ttggccatag 
     5161 ttgcccgcac cgtagtgcgc caagcaacct agtggtttat ccacattctc cggaccgtta 
     5221 atggccgtcc tcgccattca ccacaagcgc agcaaggaac gcttattgtg gtatatcccc 
     5281 gggtttgcgg tggacggggc aactcctgac gtcagtttat tttacacccc ttaacggcag 
     5341 ctgggtgaca aacaaaaaac gacagaaaac cacggtttga taccctccaa acagtggttt 
     5401 tctgtcgtcc aaaaatagcc gaaaagtgtt gacgtataca cttgttttcg gtaaaatgaa 
     5461 gacataactt aaacattgta agtgagggct tacaaaccaa gtgttcgatg ctgcaacatc 
     5521 ggacactttt tatttgtcat tctttatttg tattcaattt tgcaaatagc tcgcaaacaa 
     5581 aatatgtatc atcaaatcta ttaaccttgt tgtctgcaaa caacagggtt ttttgttgtt 
     5641 tatttagaat aactagaacc agaattcaat gccaaaactt tcacattgac ttaacttgac 
     5701 tttatcttac acgatttttt ttttgacgta aagccccggg cctgaaatca cttttctcta 
     5761 ctgatttcac tgatttcatt tttattatat aatcctcaaa tagcctgtat tcactgattt 
     5821 taaatgtgat ttcattttat tgactttagt gatataagat gctagtattg aggaaagtga 
     5881 aatcaaagga gagaataaaa atatgaatat ttctcgtatg aacgtggact ttggaaacag 
     5941 tatgtacatg aatttaattg atggttattt ttttgaattg cctacaaatg tagtagagat 
     6001 atctaaagaa gctgctgaag gaaaatttac gagtatcgtt gaagatccgg cagatttaaa 
     6061 ggaccggtta ttagtttcta cagttattga tgaaacagag agatattttc tagttggtga 
     6121 acttgctgaa ccagaagtgt taggcaacca acacatcaag aagttacata ataaagtaga 
     6181 gtcacatatt ccatacgtaa catttttagc tgcaactgct tattaccaag cgctaaaagg 
     6241 caaacgtgaa gataatgaag ttactattga atactttcaa acaatgctac caatttggct 
     6301 tcttaaaaaa ttggataagt tcagtgaaat gcagaaaagg atggcatcta aatttttggg 
     6361 cactcaccaa gtaaaggtgc tgacattagg attagaaaaa gagcttacta taaaagtgga 
     6421 agatgcagcg tgcaggatcg aatctgaagt agcaagatgg gcaataaaga aaaactttga 
     6481 cctagaagat aaagactatg ccgaacaatt taaaaattat gacgtagttt tttgtgattt 
     6541 aggtggcgga acagatgatc tagtattact accagctgga ttaaaaccgc caaaaagtcg 
     6601 tgattctttt gtttctaata ccgaagcacc gtttttagcg cacttagaaa aattgagaaa 
     6661 agaaaaactc ctagagcact ttgatagcgt tagggagctt gaaaagttta tatactcaaa 
     6721 tattggaaaa actaagatgg aacgaagaga cgggaatacc ggtcagaaat ttgatttaac 
     6781 tgatatcatc aaaaaatctc ttaaagaata cacagaaatc aaaatagccc aagctgaaaa 
     6841 tacgttccct gcaccaaaag ataaggttta caaatacctt tattttggcg gtgttggcga 
     6901 ggtgcttgaa gaatcaatta gtgtggttac tgaagagaga tatggccgtg atatttctga 
     6961 atcaaatcat atagttgctg aggatgcaag actgctcaac ttatatggcc ttgaagtttt 
     7021 aagccgcgct gaacaagtaa agaaacaggc aaatgaaaaa gaggcacaat caatttaggt 
     7081 gattagaaat ggggaaaaac aaaagaattc cactctttaa tgtccgaaca acacaaatgt 
     7141 ctgatgaaat gtacgatttt gttttagagc agattagtac attcagtaaa ggtaagagta 
     7201 agggtacctt tagagagtat gcctttcagc tcatagaaag ggacatgcaa caacagaaag 
     7261 aggaacagca aaatagagaa aaagatcgtc atgttcatga tgaattaatt gccatgagag 
     7321 aagaaatgaa aaaagaattt cgtgatttga ggaaaaaaat tgatcaggga tcgatctacg 
     7381 tagaacacaa aacagctgat ccaaagtcag cttcaaaaac gattgaagaa ggtcagttaa 
     7441 tcactgaaaa aatcactgga actattgaag aagaatacga ctatgatttt taagagcctg 
     7501 gattaatcta ggctcttttt ttatgccatt taagggagga ttgcatgaca aacttttttt 
     7561 agttgcaaca cagacgccct gagcaaccgg cggatttgtc ctactcagga gagcgttcac 
     7621 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg 
     7681 atgcctctag cacttagact cgagcggccg ctttttgaca ccagaccaac tggtaatggt 
     7741 agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag tcgtcgccac 
     7801 caatccccat atggaaaccg tcgatattca gccatgtgcc ttcttccgcg tgcagcagat 
     7861 ggcgatggct ggtttccatc agttgctgtt gactgtagcg gctgatgttg aactggaagt 
     7921 cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc agaccgtttt 
     7981 cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag cggtcaaaac 
     8041 aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc cagtttaccc 
     8101 gctctgctac ctgcgccagc tggcagttca ggccaatccg cgccggatgc ggtgtatcgc 
     8161 tcgccacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc cggtaggttt 
     8221 tccggctgat aaataaggtt ttcccctgat gctgccacgc gtgagcggtc gtaatcagca 
     8281 ccgcatcagc aagtgtatct gccgtgcact gcaacaacgc tgcttcggcc tggtaatggc 
     8341 ccgccgcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct tcacttacgc 
     8401 caatgtcgtt atccagcggt gcacgggtga actgatcgcg cagcggcgtc agcagttggt 
     8461 ttttatcgcc aatccacatc tgtgaaagag agcctgactg gcggttaaat tgccaacgct 
     8521 tattacccag ctcgatgcaa aaatccattt cgctggtggt cagatgcggg atggcgtggg 
     8581 acgcggcggg gagcgtcaca ctgaggtttt ccgccagacg ccactgctgc caggcgctga 
     8641 tgtgcccggc ttctgaccat gcggtcgcgt tcggttgcac tacgcgtact gtgagccaga 
     8701 gttgcccggc gctctccggc tgcggtagtt caggcagttc aatcaactgt ttaccttgtg 
     8761 gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc gccaccatcc 
     8821 agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact tcgatggttt 
     8881 gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc agcgctggat 
     8941 gcggcgtgcg gtcggcaaag accagaccgt tcatacagaa ctggcgatcg ttcggcgtat 
     9001 cgccaaaatc accgccgtaa gccgaccacg ggttgccgtt ttcatcatat ttaatcagcg 
     9061 actgatccac ccagtcccag acgaagccgc cctgtaaacg gggatactga cgaaacgcct 
     9121 gccagtattt agcgaaaccg ccaagactgt tacccatcgc gtgggcgtat tcgcaaagga 
     9181 tcagcgggcg cgtctctcca ggtagcgaaa gccatttttt gatggaccat ttcggcacag 
     9241 ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata tcggtggccg 
     9301 tggtgtcggc tccgccgcct tcatactgca ccgggcggga aggatcgaca gatttgatcc 
     9361 agcgatacag cgcgtcgtga ttagcgccgt ggcctgattc attccccagc gaccagatga 
     9421 tcacactcgg gtgattacga tcgcgctgca ccattcgcgt tacgcgttcg ctcatcgccg 
     9481 gtagccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg gtttcaatat 
     9541 tggcttcatc caccacatac aggccgtagc ggtcgcacag cgtgtaccac agcggatggt 
     9601 tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcatcagc aggatatcct 
     9661 gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg tgacggttaa 
     9721 cgcctcgaat cagcaacggc ttgccgttca gcagcagcag accattttca atccgcacct 
     9781 cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt 
     9841 caaccaccgc acgatagaga ttcgggattt cggcgctcca cagtttcggg ttttcgacgt 
     9901 tcagacgtag tgtgacgcga tcggcataac caccacgctc atcgataatt tcaccgccga 
     9961 aaggcgcggt gccgctggcg acctgcgttt caccctgcca taaagaaact gttacccgta 
    10021 ggtagtcacg caactcgccg cacatctgaa cttcagcctc cagtacagcg cggctgaaat 
    10081 catcattaaa gcgagtggca acatggaaat cgctgatttg tgtagtcggt ttatgcagca 
    10141 acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc agataactgc 
    10201 cgtcactcca gcgcagcacc atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc 
    10261 tcaggtcaaa ttcagacggc aaacgactgt cctggccgta accgacccag cgcccgttgc 
    10321 accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg ccttcctgta 
    10381 gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa 
    10441 caaacggcgg attgaccgta atgggatagg tcacgttggt gtagatgggc gcatcgtaac 
    10501 cgtgcatctg ccagtttgag gggacgacga cagtatcggc ctcaggaaga tcgcactcca 
    10561 gccagctttc cggcaccgct tctggtgccg gaaaccaggc aaagcgccat tcgccattca 
    10621 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 
    10681 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 
    10741 gacgttgtaa aacgacggga tctatcat 
//
