LOCUS       115_Myog'-2A-mCherryNLS-PGK-Purodtk.xdna    8352 bp      DNA     circular   8/31/2015
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=SEQUENCING:  2013-12-11 - 1703-2685: 115-1-mCh, +G1974 (OK) 
            - 2489-3332: 115-1-S347, 100% - 3097-4048: 115-1-S346, 100% 
            -                       2013-12-10 - 3918-4870: 115-1-S345, 
            100% -  -     2013-12-03 - 3344-4054: 115-4-S346, A3771G (T4
            47A) - 3918-4780: 115-4-S345, 100% -  -  - 2013-11-11 - 3831
            -4056: 115-10_S346, 100% - 3902-4512: 115-10_S345, 100% -   
                                     2013-11-14 - 1693-2590: 115-10_mCh,
             +G1974 (OK) - 2488-3339: 115-10_S347, 100% - 3172-4056: 115
            -10_S346, C3784T, A451V - 
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(4811..4828)
                     /label=M13-fwd
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     205..225
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4785..4804)
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      7561..8189
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4899..4967
                     /label=LacZ alpha
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    177..199
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             6750..7409
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     complement(1821..1854)
                     /label=LoxP
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_recomb     complement(4035..4068)
                     /label=LoxP
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     Promoter        143..172
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    964..1020
                     /label=PTV1-2A motif
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ATNFSLLKQAGDVEENPGP" \ 
     CDS             5743..6531
                     /label=Neo(r)/Kan(r)
                     /SerialCloner_Color=&h008080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4075..4749
                     /label=Myog right arm
                     /SerialCloner_Color=&h400000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from Myog right arm.xdna [675  nt] : (AscI[7] / NotI[686]) \ 
     misc_feature    257..958
                     /label=Myog left arm
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     STS             4077..4674
                     /label=STS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=/standard_name="Myog" - /db_xref="UniSTS:265671" - 1092..1689 \ 
     STS             4292..4609
                     /label=STS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=/standard_name="PMC151607P2" - /db_xref="UniSTS:271152" - 2008..2325 \ 
     CDS             1021..1728
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     sig_peptide     1729..1749
                     /label=NLS
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PKKKRKV" \ 
     sig_peptide     1756..1776
                     /label=NLS
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PKKKRKV" \ 
     exon            258..339
                     /label=Ex2
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             258..339
                     /label=Myog
                     /SerialCloner_Color=&h004080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     STS             260..857
                     /label=STS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=/standard_name="Myog" - /db_xref="UniSTS:265671" - 1092..1689 \ 
     misc_feature    839..957
                     /label=Ex3'
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2433..4034
                     /label=PuroDeltaTk
                     /SerialCloner_Color=&h743DE1
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     promoter        1913..2251
                     /label=mPGK
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatcCgtg cccagtgaat gcaactccca cagcgcctcc tgcagtccgg 
      301 agtggggcaa tgcactggag ttcggtccca acccaggagg taagtgaatc ctgaccctgg 
      361 taacatggct caaatccctt agtccctacc taggctgggc ttgtaccctt accataagcc 
      421 ctgtcccaat cacccttcag cagcttttct aggaacttgc tgacctgagg gccccaaatg 
      481 gtgggctgga gaccctgtgc ctcccggtag ccttgaccag ctaaagagta gtcagagcct 
      541 ccagcaactt ccctgctggt ctctgacctc agagagaaga gcctgctcct gccccagggg 
      601 tccctgggga tcactcagtc agtgttgtaa aactgtgccc tgtgccctag gatatcctgg 
      661 cttcacctag ggatagccac ttttaaaacc ccacaagcac acacacagca tggggagtac 
      721 tagtgtcctg ggggcgggga acactcctga gtcttcctta cccattttgt cctggaaaat 
      781 ctgtgtcaag ggtcccatac cagttgccca ggaaacattc tcccactttt tcttgcagat 
      841 catttgctcg cggctgaccc tacagacgcc cacaatctgc actcccttac gtccatcgtC 
      901 gacagcatca cggtggagga tatgtctgtt gccttcccag acgaaaccat gcccaacGct 
      961 agcgccacta acttctccct gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca 
     1021 ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT GCGCTTCAAG 
     1081 GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC 
     1141 CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC 
     1201 TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA CGTGAAGCAC 
     1261 CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC 
     1321 GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC 
     1381 GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA CGGCCCCGTA 
     1441 ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC CGAGGACGGC 
     1501 GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCT 
     1561 GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC 
     1621 AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA 
     1681 CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGCC CAAGAAGAAG 
     1741 AGGAAGGTGg gtggcCCTAA GAAAAAGAGA AAGGTGtgac gtacaagtaa GAATTCcgat 
     1801 catattcaat aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc 
     1861 tgaagaggag tttacgtcca gccaagctta ggatctcgac ctcgaaattc taccgggtag 
     1921 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctggcacttg 
     1981 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggTA GGCGCCAACC 
     2041 GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT 
     2101 CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC 
     2161 TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG 
     2221 CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC 
     2281 CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG 
     2341 CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC 
     2401 TCCGGGCCTT TCGACCTGCA GCCAACGCCA CCATGGGGAC CGAGTACAAG CCCACGGTGC 
     2461 GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG 
     2521 ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC 
     2581 TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG 
     2641 ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG 
     2701 CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA 
     2761 TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG 
     2821 GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG 
     2881 AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC 
     2941 CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC 
     3001 GCACCTGGTG CATGACCCGC AAGCCCGGTG CCGGATCCAT GCCCACGCTA CTGCGGGTTT 
     3061 ATATAGACGG TCCTCACGGG ATGGGGAAAA CCACCACCAC GCAACTGCTG GTGGCCCTGG 
     3121 GTTCGCGCGA CGATATCGTC TACGTACCCG AGCCGATGAC TTACTGGCAG GTGCTGGGGG 
     3181 CTTCCGAGAC AATCGCGAAC ATCTACACCA CACAACACCG CCTCGACCAG GGTGAGATAT 
     3241 CGGCCGGGGA CGCGGCGGTG GTAATGACAA GCGCCCAGAT AACAATGGGC ATGCCTTATG 
     3301 CCGTGACCGA CGCCGTTCTG GCTCCTCATA TCGGGGGGGA GGCTGGGAGC TCACATGCCC 
     3361 CGCCCCCGGC CCTCACCCTC ATCTTCGACC GCCATCCCAT CGCCGCCCTC CTGTGCTACC 
     3421 CGGCCGCGCG ATACCTTATG GGCAGCATGA CCCCCCAGGC CGTGCTGGCG TTCGTGGCCC 
     3481 TCATCCCGCC GACCTTGCCC GGCACAAACA TCGTGTTGGG GGCCCTTCCG GAGGACAGAC 
     3541 ACATCGACCG CCTGGCCAAA CGCCAGCGCC CCGGCGAGCG GCTTGACCTG GCTATGCTGG 
     3601 CCGCGATTCG CCGCGTTTAC GGGCTGCTTG CCAATACGGT GCGGTATCTG CAGGGCGGCG 
     3661 GGTCGTGGCG GGAGGATTGG GGACAGCTTT CGGGGACGGC CGTGCCGCCC CAGGGTGCCG 
     3721 AGCCCCAGAG CAACGCGGGC CCACGACCCC ATATCGGGGA CACGTTATTT ACCCTGTTTC 
     3781 GGGCCCCCGA GTTGCTGGCC CCCAACGGCG ACCTGTACAA CGTGTTTGCC TGGGCCTTGG 
     3841 ACGTCTTGGC CAAACGCCTC CGTCCCATGC ACGTCTTTAT CCTGGATTAC GACCAATCGC 
     3901 CCGCCGGCTG CCGGGACGCC CTGCTGCAAC TTACCTCCGG GATGGTCCAG ACCCACGTCA 
     3961 CCACCCCCGG CTCCATACCG ACGATCTGCG ACCTGGCGCG CACGTTTGCC CGGGAGATGG 
     4021 GGGAGGCTAA CTGAataact tcgtataatg tatgctatac gaagttatGG cgcgCCgatt 
     4081 gtctgtcagg ctgggtgtgc atgtgagccc ccaagttggt gtcaaaagcc atcacttctg 
     4141 tagcaggggg cttttaagtg gggctgtcct gatgtccaga aaacagccct gggctgccac 
     4201 aagccagact ccccactccc cattcacata aggctaacac ccagcccagc gagggaattt 
     4261 agctgactcc ttaaagcaga gagcatcctc ttctgaggag agaaagatgg agtccagaga 
     4321 gcccccttgt taatgtccct cagtggggca aactcaggag cttctttttt gtttatcata 
     4381 atatgcctcg aattccaccc cccaccccca aaatgaaacc gtttgagaga catgagtgcc 
     4441 ctgacctgga caagtgtgca catctgttct agtctcttcc tgaagccagt ggctgggctg 
     4501 ggcctgccct gagttgagag agaaggggga ggagctatcc ggttccaaag cctctggggg 
     4561 ccaagcattt gcagtggatc ttgggaacct tccagtgctt tgtgtattgt ttattgtttt 
     4621 gtgtgttgtt tgtaaagctg ccgtctgacc aaggtctcct gtgctgatga taccgggaac 
     4681 aggcagggaa gggggtgggg gctcttgggg tgacttcttt tgttaactaa gcattgtgtg 
     4741 gttttgcGCg gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt 
     4801 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 
     4861 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 
     4921 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag 
     4981 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     5041 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     5101 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     5161 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     5221 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     5281 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
     5341 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
     5401 caaaattcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag 
     5461 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca 
     5521 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg 
     5581 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg 
     5641 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga 
     5701 tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 
     5761 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 
     5821 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 
     5881 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 
     5941 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 
     6001 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atcccacctt 
     6061 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 
     6121 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 
     6181 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 
     6241 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 
     6301 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 
     6361 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 
     6421 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 
     6481 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattgaa 
     6541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
     6601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
     6661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
     6721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
     6781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
     6841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
     6901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
     6961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
     7021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
     7081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
     7141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
     7201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
     7261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
     7321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
     7381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
     7441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
     7501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
     7561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
     7621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
     7681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 
     7741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
     7801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
     7861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
     7921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
     7981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
     8041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
     8101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
     8161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
     8221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
     8281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
     8341 aggaagcgga ag
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