LOCUS       117_Myog'-2A-mCherry-NLS-PGK-Puro.txt.xdna    7948 bp      DNA     circular   6/5/2014
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=SEQUENCING  2013-10-09 - 368-1024: 100%, 117-11-S326 - 
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(4407..4424)
                     /label=M13-fwd
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     205..225
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4381..4400)
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      7157..7785
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4495..4563
                     /label=LacZ alpha
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    177..199
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             6346..7005
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_recomb     complement(1821..1854)
                     /label=LoxP
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_recomb     complement(3607..3640)
                     /label=LoxP
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     Promoter        143..172
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    964..1020
                     /label=PTV1-2A motif
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="ATNFSLLKQAGDVEENPGP" \ 
     CDS             2451..3051
                     /label=Puro(r)
                     /SerialCloner_Color=&h004000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             5339..6127
                     /label=Neo(r)/Kan(r)
                     /SerialCloner_Color=&h008080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3671..4345
                     /label=Myog right arm
                     /SerialCloner_Color=&h400000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from Myog right arm.xdna [675  nt] : (AscI[7] / NotI[686]) \ 
     misc_feature    257..958
                     /label=Myog left arm
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     STS             3673..4270
                     /label=STS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=/standard_name="Myog" - /db_xref="UniSTS:265671" - 1092..1689 \ 
     STS             3888..4205
                     /label=STS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=/standard_name="PMC151607P2" - /db_xref="UniSTS:271152" - 2008..2325 \ 
     CDS             1021..1728
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     sig_peptide     1729..1749
                     /label=NLS
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PKKKRKV" \ 
     sig_peptide     1756..1776
                     /label=NLS
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PKKKRKV" \ 
     exon            258..339
                     /label=Ex2
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             258..339
                     /label=Myog
                     /SerialCloner_Color=&h004080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     STS             260..857
                     /label=STS
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=/standard_name="Myog" - /db_xref="UniSTS:265671" - 1092..1689 \ 
     misc_feature    839..957
                     /label=Ex3'
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1909..2251
                     /label=mPGK promoter
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 
      241 gtaccgagct cggatcCgtg cccagtgaat gcaactccca cagcgcctcc tgcagtccgg 
      301 agtggggcaa tgcactggag ttcggtccca acccaggagg taagtgaatc ctgaccctgg 
      361 taacatggct caaatccctt agtccctacc taggctgggc ttgtaccctt accataagcc 
      421 ctgtcccaat cacccttcag cagcttttct aggaacttgc tgacctgagg gccccaaatg 
      481 gtgggctgga gaccctgtgc ctcccggtag ccttgaccag ctaaagagta gtcagagcct 
      541 ccagcaactt ccctgctggt ctctgacctc agagagaaga gcctgctcct gccccagggg 
      601 tccctgggga tcactcagtc agtgttgtaa aactgtgccc tgtgccctag gatatcctgg 
      661 cttcacctag ggatagccac ttttaaaacc ccacaagcac acacacagca tggggagtac 
      721 tagtgtcctg ggggcgggga acactcctga gtcttcctta cccattttgt cctggaaaat 
      781 ctgtgtcaag ggtcccatac cagttgccca ggaaacattc tcccactttt tcttgcagat 
      841 catttgctcg cggctgaccc tacagacgcc cacaatctgc actcccttac gtccatcgtC 
      901 gacagcatca cggtggagga tatgtctgtt gccttcccag acgaaaccat gcccaacGct 
      961 agcgccacta acttctccct gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca 
     1021 ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT GCGCTTCAAG 
     1081 GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC 
     1141 CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC 
     1201 TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA CGTGAAGCAC 
     1261 CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC 
     1321 GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC 
     1381 GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA CGGCCCCGTA 
     1441 ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC CGAGGACGGC 
     1501 GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCT 
     1561 GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC 
     1621 AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA 
     1681 CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGCC CAAGAAGAAG 
     1741 AGGAAGGTGg gtggcCCTAA GAAAAAGAGA AAGGTGtgac gtacaagtaa GAATTCcgat 
     1801 catattcaat aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc 
     1861 tgaagaggag tttacgtcca gccaagctta ggatctcgac ctcgaaattc taccgggtag 
     1921 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctggcacttg 
     1981 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta gcgccaaccg 
     2041 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc 
     2101 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact 
     2161 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc 
     2221 ggccaatagc agctttgctc cttcgctttc tgggctcagc agctgggaag ggtgggtccg 
     2281 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc 
     2341 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc 
     2401 cgggcctttc gacctgcatc catctagatc tcgagcagct gaagcttacc atgaccgagt 
     2461 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 
     2521 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 
     2581 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 
     2641 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 
     2701 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 
     2761 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 
     2821 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 
     2881 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 
     2941 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 
     3001 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc 
     3061 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg atatcagatc 
     3121 cccgggatgc agaaattgat gatctattaa acaataaaga tgtccactaa aatggaagtt 
     3181 tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt gaatggaagg 
     3241 attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc tttactgaag 
     3301 gctctttact attgctttat gataatgttt catagttgga tatcataatt taaacaagca 
     3361 aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct atagatctct 
     3421 cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact gtggtttcca 
     3481 aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca catacacttc 
     3541 attctcagta ttgttttgcc aagttctaat tccatcagaa gctggtcgag atccggaacc 
     3601 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtccctc gaagaggttc 
     3661 actaGGcgcg CCgattgtct gtcaggctgg gtgtgcatgt gagcccccaa gttggtgtca 
     3721 aaagccatca cttctgtagc agggggcttt taagtggggc tgtcctgatg tccagaaaac 
     3781 agccctgggc tgccacaagc cagactcccc actccccatt cacataaggc taacacccag 
     3841 cccagcgagg gaatttagct gactccttaa agcagagagc atcctcttct gaggagagaa 
     3901 agatggagtc cagagagccc ccttgttaat gtccctcagt ggggcaaact caggagcttc 
     3961 ttttttgttt atcataatat gcctcgaatt ccacccccca cccccaaaat gaaaccgttt 
     4021 gagagacatg agtgccctga cctggacaag tgtgcacatc tgttctagtc tcttcctgaa 
     4081 gccagtggct gggctgggcc tgccctgagt tgagagagaa gggggaggag ctatccggtt 
     4141 ccaaagcctc tgggggccaa gcatttgcag tggatcttgg gaaccttcca gtgctttgtg 
     4201 tattgtttat tgttttgtgt gttgtttgta aagctgccgt ctgaccaagg tctcctgtgc 
     4261 tgatgatacc gggaacaggc agggaagggg gtgggggctc ttggggtgac ttcttttgtt 
     4321 aactaagcat tgtgtggttt tgcGCggccg ctcgagcatg catctagagg gcccaattcg 
     4381 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa 
     4441 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 
     4501 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 
     4561 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     4621 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     4681 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     4741 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     4801 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     4861 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     4921 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     4981 ttaacgcgaa ttttaacaaa attcagggcg caagggctgc taaaggaagc ggaacacgta 
     5041 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg 
     5101 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg 
     5161 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc 
     5221 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat 
     5281 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat 
     5341 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 
     5401 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 
     5461 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 
     5521 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 
     5581 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 
     5641 cctgtcatcc caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 
     5701 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 
     5761 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 
     5821 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 
     5881 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 
     5941 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 
     6001 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 
     6061 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 
     6121 gttcttctga attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 
     6181 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 
     6241 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 
     6301 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 
     6361 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 
     6421 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 
     6481 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 
     6541 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 
     6601 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 
     6661 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 
     6721 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 
     6781 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 
     6841 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 
     6901 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 
     6961 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 
     7021 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     7081 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     7141 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     7201 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     7261 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     7321 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     7381 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     7441 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     7501 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     7561 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     7621 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     7681 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     7741 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     7801 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 
     7861 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 
     7921 agcgagtcag tgagcgagga agcggaag
//