LOCUS       Exported                2271 bp ds-DNA    circular SYN 17-SEP-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    taRAJ31minNORS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 17 Sep 2015 from SnapGene 2.3.2
            http://www.snapgene.com
COMMENT     taRAJ31-PCRprod-withoutrestsite from 1 to 261
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     5..22
                     /label="M13-fwd"
                     /note="M13-fwd"
     misc_feature    6..22
                     /label="M13 forward"
                     /note="M13 forward"
     misc_feature    197..250
                     /label="PLtetO1"
                     /note="PLtetO1-2"
     misc_feature    join(251..272,279..320)
                     /label="cirRAJ31min"
                     /note="cirRAJ31min"
     misc_feature    join(251..272,279..320)
                     /label="RAJ31min_trans"
                     /note="RAJ31min_trans"
     misc_feature    273..278
                     /label="Toehold"
                     /note="Toehold"
     misc_feature    321..349
                     /label="rrnC terminator"
                     /note="rrnC terminator"
     misc_feature    622..1482
                     /label="AmpR"
                     /note="AmpR"
     misc_feature    622..625
                     /label="New Feature"
                     /note="New Feature"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc gaattctccc tatcagtgat agagattgac atccctatca gtgatagaga
      241 tactgagcac ggatcaatat taagctttac cgtcgtctca tccgcaatta agtccctatc
      301 cgctgtcact gtattggtct atccttagcg aaagctaagg attttttttc tgcagggatc
      361 gacgagagca gcgcgactgg atcagttctg gacgagcgag ctgtcgtccg acccgtgatc
      421 ttacggcatt atacgtatga tcggtccacg atcagctaga ttatctagtc agcttgatgt
      481 catagctgtt tcctgaggct caatactgac catttaaatc atacctgacc tccatagcag
      541 aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact
      601 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
      661 gagctaagga agctaaaatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      721 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      781 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      841 ttgagagttt acgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      901 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      961 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctc acggatggca
     1021 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1081 tacttctggc aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1141 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1201 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1261 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1321 caggatcact tctgcgctcg gccctcccgg ctggctggtt tattgctgat aaatctggag
     1381 ccggtgagcg tgggactcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1441 gcatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1501 tcgctgagat aggtgcctca ctgattaagc attggtaatg agggcccaaa tgtaatcacc
     1561 tggctcacct tcgggtgggc ctttcttgag gacctaaatg taatcacctg gctcaccttc
     1621 gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1681 acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2101 gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg
     2161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2221 aaaaaaagga tctcaagaag atcctttgat tttctaccga agaaaggccc a
//
