LOCUS       Exported                2320 bp ds-DNA     circular SYN 28-FEB-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Assembled_Lasso1_pSB1C3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2016 from SnapGene 3.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4
                     /label=pSB1C3-DB Start
                     /note="pSB1C3-DB Start"
     misc_feature    5..24
                     /label=pT7
                     /note="pT7"
     misc_feature    complement(103..124)
                     /label=Target TNF
                     /note="Target TNF"
     misc_feature    complement(join(125..133,135..141,143..146,148..151))
                     /label=Spacing region (Can be changed)
                     /note="Spacing region"
     misc_feature    152..169
                     /label=PIE
                     /note="PIE"
     misc_feature    complement(join(170..179,181..184,186..191,193..196))
                     /label=Spacer (can be changed)
                     /note="Spacer"
     misc_feature    219..266
                     /label=T7 Terminator
                     /note="T7 Terminator"
     misc_feature    267..275
                     /label=pSB1C3-DB End
                     /note="pSB1C3-DB End"
     misc_feature    522..1136
                     /note="pUC19-derived pMB1"
                     /note="copy number of 100-300 per cell"
     misc_feature    1421..2080
                     /label=CAT (CamR)
                     /note="CAT"
     misc_feature    2310..2315
                     /label=EcoRI
                     /note="EcoRI"
     misc_feature    2320
                     /label=Overlap
                     /note="Overlap"
ORIGIN
        1 cgcttaatac gactcactat agggaggctg tcctcgtccg tatgacgaga gaagcctacc
       61 agagaaacac acgacgtaag tcgtggtaca ttacctggta cagttctctt caagggacaa
      121 ggctaacaac aactaacaac atacaatcaa cataatttgc catctgggta acaacaacat
      181 acaatcaaca atcaaccaag gctgtcctcg tcgatatcct agcataaccc cttggggcct
      241 ctaaacgggt cttgaggggt tttttgagcc tcttcctgca gtccggcaaa aaaacggcaa
      301 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc
      361 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      421 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      481 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      541 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      601 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      661 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      721 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      781 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      841 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      901 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      961 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1021 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1081 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1141 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1201 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1261 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa
     1321 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1381 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1441 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1501 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1561 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1621 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1681 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1741 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1801 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1861 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1921 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1981 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2041 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2101 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     2161 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
     2221 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     2281 tccttagctt tcgctaagga tgatttctgg aattcgaaga
//
