LOCUS       pSCKtheoRAJ12_3         4542 bp ds-DNA     circular     04-DEC-2014
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1180..1191
                     /label=B0042_Stop codon in 6 frames
                     /ApEinfo_fwdcolor=#ffbc01
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1192..1260
                     /label=B0054_terminator derived from tonB ter
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1261..1280
                     /label=G00102_VR annealing site
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    217..270
                     /label=PLtetR
                     /ApEinfo_fwdcolor=#37ff42
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1281..1321)
                     /label=B0062_Terminator from rrnC operon(reverse)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    289..343
                     /label=pLlacO-1
                     /ApEinfo_fwdcolor=#14ffe7
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1332..2295)
                     /label=P1003_KanR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=#a4ff2b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    142..166
                     /label=theo aptamer
                     /ApEinfo_fwdcolor=#e2ff1e
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2304..4340
                     /label=I50042_pSC101 low copy replication origin
                     /ApEinfo_fwdcolor=#4a7eff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4348..4419
                     /label=B0053_Terminator derived from hisI ter
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4420..4439
                     /label=G00100_VF2 annealing site
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4440..4517)
                     /label=B0055_Terminator from phage T3 ter (reverse)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4518..4529
                     /label=B0042_Stop codon in 6 frames(1)
                     /ApEinfo_label=B0042_Stop codon in 6 frames
                     /ApEinfo_fwdcolor=#ffbc01
                     /ApEinfo_revcolor=#ffbc01
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    405..1160
                     /label=sfGFP-LAA
                     /ApEinfo_fwdcolor=#11ff33
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1122..1154
                     /label=LAA
                     /ApEinfo_fwdcolor=#03ffc9
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    139..169
                     /label=aptamer
                     /ApEinfo_fwdcolor=#eeff0e
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    171..171
                     /label=M
                     /ApEinfo_fwdcolor=#ff73f2
                     /ApEinfo_revcolor=#ff73f2
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 gaattcgcgg ccgcttctag agACAGAAAA GCCCGCCTTT CGGCGGGCTT TGCCCAGAAT
       61 CCCTTCGAGC AAATCCTAGC GAGGTGCCGC TAGATTCGCT CAAAGGAACC CTTCTTCCTG
      121 CCCGTCTGGA ATCCAGGACA CCTGCCAAGG GCCTTTCGGC TGGTATGTAT CTCGTCCTAT
      181 TTGGGACTCA TCAGCTGGAT GTACGAAGGC AGCGCCGTGC TCAGTATCTC TATCACTGAT
      241 AGGGATGTCA ATCTCTATCA CTGATAGGGA gctagccaat tgatgcatat aaatgtgagc
      301 ggataacatt gacattgtga gcggataaca agatactgag cacACCCAGT ATCATTCTCT
      361 TCTTCCTGCC CACGCGGAAA GAGGAGAAAG GTGTAATGac tagtCGTAAA GGCGAAGAGC
      421 TGTTCACTGG TGTCGTCCCT ATTCTGGTGG AACTGGATGG TGATGTCAAC GGTCATAAGT
      481 TTTCCGTGCG TGGCGAGGGT GAAGGTGACG CAACTAATGG TAAACTGACG CTGAAGTTCA
      541 TCTGTACTAC TGGTAAACTG CCGGTACCTT GGCCGACTCT GGTAACGACG CTGACTTATG
      601 GTGTTCAGTG CTTTGCTCGT TATCCGGACC ATATGAAGCA GCATGACTTC TTCAAGTCCG
      661 CCATGCCGGA AGGCTATGTG CAGGAACGCA CGATTTCCTT TAAGGATGAC GGCACGTACA
      721 AAACGCGTGC GGAAGTGAAA TTTGAAGGCG ATACCCTGGT AAACCGCATT GAGCTGAAAG
      781 GCATTGACTT TAAAGAAGAC GGCAATATCC TGGGCCATAA GCTGGAATAC AATTTTAACA
      841 GCCACAATGT TTACATCACC GCCGATAAAC AAAAAAATGG CATTAAAGCG AATTTTAAAA
      901 TTCGCCACAA CGTGGAGGAT GGCAGCGTGC AGCTGGCTGA TCACTACCAG CAAAACACTC
      961 CAATCGGTGA TGGTCCTGTT CTGCTGCCAG ACAATCACTA TCTGAGCACG CAAAGCGTTC
     1021 TGTCTAAAGA TCCGAACGAG AAACGCGATC ATATGGTTCT GCTGGAGTTC GTAACCGCAG
     1081 CGGGCATCAC GCATGGTATG GATGAACTGT ACAAAGCTAG CGCAGCGAAC GACGAAAATT
     1141 ACGCCCTTGC AGCGTAATAA ctgcaggagt cactaagggt tagttagtta gattagcaga
     1201 aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt tatcattggg
     1261 gctcactcaa aggcggtaat cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     1321 tgctagtatt attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1381 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1441 aggcagttcc aaagaatggc aaggtcctgg taacggtctg cgattccgac ccgtccaaca
     1501 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     1561 tgagtgacga ctgaatccgg tgagaatggc aagagcttgt gcatttcttt ccagacttgt
     1621 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     1681 atgcgtgatt gcgcctgagc aagacgaaat acacgatcgc tgttaaaagg acaattacaa
     1741 acaggaatcg aatgtaaccg gcgcaggaac acggccagcg catcaacaat attttcacct
     1801 gaatcaggat attcttctaa tacctggaag gctgttttcc caggaatcgc ggtggtgagt
     1861 aaccacgcat catcaggagt acggataaaa tgcttgatgg tcgggagagg cataaactcc
     1921 gtcagccagt tgagacggac catctcatct gtaacatcat tggcaacgct acctttgcca
     1981 tgtttcagaa acaactctgg cgcatcgggc ttcccataca agcgatagat tgtcgcacct
     2041 gattgcccga cattatcgcg agcccattta tacccatata aatcagcgtc catgttggag
     2101 tttaagcgcg gacgggagca agacgtttcc cgttgaatat ggctcataac accccttgta
     2161 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     2221 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
     2281 gagttgaagg atcagctcta gtagttacat tgtcgatctg ttcatggtga acagctttaa
     2341 atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg
     2401 tgcatatgga cagttttccc tttgatatct aacggtgaac agttgttcta cttttgtttg
     2461 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat
     2521 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     2581 ttgagatcat gcttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     2641 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct
     2701 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     2761 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     2821 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     2881 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     2941 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     3001 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     3061 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     3121 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     3181 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     3241 ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta
     3301 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     3361 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     3421 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     3481 taatcactat accaattgag atgggctagt caatgataat tacatgtcct tttcctttga
     3541 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     3601 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     3661 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     3721 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     3781 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc
     3841 aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg
     3901 tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag
     3961 gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg
     4021 cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca
     4081 gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat
     4141 tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac
     4201 tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     4261 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     4321 tcgtaaactt ggtctgacag ctctagctcc ggcaaaaaaa cgggcaaggt gtcaccaccc
     4381 tgcccttttt ctttaaaacc gaaaagatta cttcgcgttt gccacctgac gtctaagaaa
     4441 aggaatattc agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg tgagccagtg
     4501 agttgattgc tacgtaatta gttagttagc ccttagtgac tc 
//
